A5050458563- Advanced Database Systems and Queries
- Bayesian Modeling and Causal Inference
- Data Mining Algorithms and Applications
- Acute Myeloid Leukemia Research
- Single-cell and spatial transcriptomics
- Hematopoietic Stem Cell Transplantation
- Chronic Lymphocytic Leukemia Research
- AI-based Problem Solving and Planning
- Acute Lymphoblastic Leukemia research
- Multiple Myeloma Research and Treatments
- Error Correcting Code Techniques
- Ecology and Conservation Studies
- Lymphoma Diagnosis and Treatment
- Hematological disorders and diagnostics
- Protein Degradation and Inhibitors
- CAR-T cell therapy research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Calcium signaling and nucleotide metabolism
- Immune Cell Function and Interaction
- Lung Cancer Research Studies
- Bone and Joint Diseases
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Advanced Data Storage Technologies
- Cancer Genomics and Diagnostics
Erasmus MC
2013-2023
Instituto Português de Oncologia Francisco Gentil
2020
Erasmus University Rotterdam
2013-2019
Massachusetts General Hospital
2019
John Wiley & Sons (United States)
2019
Hudson Institute
2019
Background While it is known that CD123 normally strongly expressed on plasmacytoid dendritic cells and completely absent nucleated red blood cells, detailed information regarding expression in acute leukemia scarce and, if available, hard to compare due different methodologies. Methods was evaluated using standardized EuroFlow immunophenotyping 139 pediatric AML, 316 adult 193 BCP‐ALL, 69 101 T‐ALL, 28 T‐ALL patients. Paired diagnosis‐relapse samples were available for 57 AML 19 BCP‐ALL...
Background Acute myeloid leukemia (AML) is a hematopoietic malignancy which biologically, phenotypically and genetically very heterogeneous. Outcome of patients with AML remains dismal, highlighting the need for improved, less toxic therapies. Chimeric antigen receptor T-cell (CART) immunotherapies refractory or relapse (R/R) are challenging because absence universal pan-AML target shared expression antigens normal stem/progenitor cells (HSPCs), may lead to life-threating on-target/off-tumor...
Precise classification of acute leukemia (AL) is crucial for adequate treatment. EuroFlow has previously designed an AL orientation tube (ALOT) to guide towards the relevant panel (T-cell lymphoblastic (T-ALL), B-cell precursor (BCP)-ALL and/or myeloid (AML)) and final diagnosis. Now we built a reference database with 656 typical samples (145 T-ALL, 377 BCP-ALL, 134 AML), processed analyzed via standardized protocols. Using principal component analysis (PCA)-based plots automated algorithms...
Acute megakaryoblastic leukemia (AMKL) is a rare and heterogeneous subtype of acute myeloid (AML). We evaluated the immunophenotypic profile 72 AMKL 114 non-AMKL AML patients using EuroFlow panel. Univariate multivariate/multidimensional analyses were performed to identify most relevant markers contributing diagnosis AMKL. subdivided into transient abnormal myelopoiesis (TAM), associated with Down syndrome (ML-DS), AML-not otherwise specified megakaryocytic differentiation (NOS-AMKL),...
Precise classification of acute leukemia (AL) is crucial for adequate treatment. EuroFlow has previously designed an AL orientation tube (ALOT) to guide toward the relevant panel and final diagnosis. In this study, we validated algorithm automated (database-supported) gating identification (AGI tool) cell subsets within samples stained with ALOT. A reference database normal peripheral blood (PB, n = 41) bone marrow (BM; 45) analyzed ALOT was constructed, served as a AGI tool automatically...
Summary Given its myeloid‐restricted expression, myeloperoxidase (MPO) is typically used for lineage assignment (myeloid vs. lymphoid) during acute leukaemia (AL) diagnostics. In the present study, a robust flow cytometric definition MPO positivity was established based on standardised EuroFlow protocols, Acute Leukaemia Orientation Tube and 1734 multicentre AL cases (with confirmed assay stability). The best diagnostic performance achieved by defining as ≥20% of cells exceeding...
Summary B‐cell precursors ( BCP ) regeneration in bone marrow BM after induction chemotherapy is prognostic for good treatment response adult acute myeloid leukaemia AML ). We detected 81% of 59 paediatric patients at first complete remission; this compared to 46% an study. did not correlate with outcome or minimal residual disease levels. In 36 healthy controls, levels were significantly higher children as adults. Therefore, does reflect , possibly due the relatively high base‐line children.
Flowcytometric analysis allows for detailed identification and characterization of large numbers cells in blood, bone marrow, other body fluids tissue samples therefore contributes to the diagnostics hematological malignancies. Novel data tools allow multidimensional comparison patient with reference databases normal, reactive, and/or leukemia/lymphoma samples. Building such requires strict quality assessment (QA) procedures. Here, we compiled a dataset developed QA methodology EuroFlow...
Nowadays, most cytometrists apply lossless compression by storing their FCS files in ZIP archives. Unfortunately, only achieves modest space savings cytometric data, due to DEFLATE being used as the underlying algorithm (LCA). Presumably, other modern LCA can outperform DEFLATE, especially terms of savings. Twenty-one codecs (programs implementing LCA) were evaluated 167,131 publicly available files. Within floating-point produced instruments, favorable ratios (CRs) achieved ZPAQ (median...
A flow cytometry score (FCS) based on four parameters has been proposed for analysis of myelodysplastic syndromes patients with <5% blasts. We evaluated whether bone marrow aspirate samples isolated from femoral heads could be used verification cutoff values the individual (IPS), contributing to FCS and compared applicability in two centers.Bone cells were obtained who underwent hip replacement surgery. The 4 cell independently centers, using their own facilities methods. Flow data files...
Abstract When it comes to data storage, the field of flow cytometry is fairly standardized, thanks standard (FCS) file format. The structure FCS files described in specification. Software that strictly complies with specification guaranteed be interoperable (in terms exchange via files). Nowadays, software interoperability crucial for eco system, as are frequently shared, and workflows rely on more than one piece (e.g., acquisition analysis software). Ideally, developers follow...