A5076501930- Cystic Fibrosis Research Advances
- Blood disorders and treatments
- Neonatal Respiratory Health Research
- Immunodeficiency and Autoimmune Disorders
- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Virus-based gene therapy research
- Pneumocystis jirovecii pneumonia detection and treatment
- Antimicrobial Peptides and Activities
- Tracheal and airway disorders
- Pediatric health and respiratory diseases
- Acute Myeloid Leukemia Research
- Respiratory viral infections research
- Sphingolipid Metabolism and Signaling
- Neonatal Health and Biochemistry
- RNA Interference and Gene Delivery
- Helicobacter pylori-related gastroenterology studies
- Advanced biosensing and bioanalysis techniques
- Synthesis and biological activity
- Cholesterol and Lipid Metabolism
- Asthma and respiratory diseases
- Drug Transport and Resistance Mechanisms
- Andrographolide Research and Applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- DNA and Nucleic Acid Chemistry
Azienda Ospedaliera Universitaria Integrata Verona
2005-2020
Laboratory of Molecular Genetics
2007-2019
University of Verona
2007-2018
University of Ferrara
2006
Université de Poitiers
2006
Centre National de la Recherche Scientifique
2006
Cystic Fibrosis Research Foundation
1993-2006
Casa Sollievo della Sofferenza
1992
Abstract Recent data show that proinflammatory stimuli may modify significantly ion transport in the airway epithelium and therefore properties of surface fluid. We have studied effect IL-4, a cytokine involved pathogenesis asthma, on transepithelial human bronchial vitro. Incubation polarized epithelial cells with IL-4 for 6–48 h causes marked inhibition amiloride-sensitive Na+ channel as measured short circuit current experiments. On other hand, evokes 2-fold increase activated by cAMP...
ABSTRACT The innate immune response to adenovirus (Ad)-derived gene transfer vectors has been shown initiate immediately after interaction of Ad with respiratory epithelial cells, through the induction extracellular signal-regulated kinase 1 and 2 (ERK1/2) JNK mitogen-activated protein (MAPK), nuclear factor κB (NF-κB), different proinflammatory genes. serotypes or 5 (Ad2/5) enter epithelia initial binding fiber coxsackievirus-adenovirus receptor (CAR) or, alternatively, cell surface heparan...
Abstract Background The transcription factor NF-kappaB is a very interesting target molecule for the design on anti-tumor, anti-inflammatory and pro-apoptotic drugs. However, application of widely-used molecular docking computational method virtual screening chemical libraries not yet reported in literature. Docking studies dataset 27 molecules from extracts two different medicinal plants to NF-kappaB-p50 were performed with purpose developing protocol fit under study. Results We enhanced...
Chronic pulmonary inflammation in patients affected by cystic fibrosis (CF) is characterized massive bronchial infiltrates of neutrophils, which sustained the interaction pathogens (e.g., Pseudomonas aeruginosa) with surface cells. To explore new treatment options focused on reduction neutrophil chemotaxis, we applied transcription factor (TF) decoy approach, based intracellular delivery double-stranded oligodeoxynucleotides (ODNs) causing inhibition binding TF-related proteins to different...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Shwachman-Diamond syndrome (SDS) is a rare autosomal recessive disease, mainly characterized by exocrine pancreatic insufficiency, hematological dysfunction and skeletal abnormalities. The SDS disease locus was mapped to chromosome 7q11 disease-associated mutations were reported in the Shwachman-Bodian-Diamond (SBDS) gene. SBDS member of highly conserved protein family with putative orthologs diverse species including archaea eukaryotes. It widely expressed many tissues its function still...
Chronic inflammatory response in the airway tract of patients affected by cystic fibrosis is characterized an excessive recruitment neutrophils to bronchial lumina, driven chemokine interleukin (IL)-8. We previously found that 5-methoxypsoralen reduces Pseudomonas aeruginosa-dependent IL-8 transcription epithelial cell lines, with IC(50) 10 μM (Nicolis E, Lampronti I, Dechecchi MC, Borgatti M, Tamanini A, Bezzerri V, Bianchi N, Mazzon Mancini Giri MG, Rizzotti P, Gambari R, Cabrini G. Int...
Abstract Respiratory insufficiency is the major cause of morbidity and mortality in patients affected by cystic fibrosis (CF). An excessive neutrophilic inflammation, mainly orchestrated release IL-8 from bronchial epithelial cells amplified chronic bacterial infection with Pseudomonas aeruginosa, leads to progressive tissue destruction. The anti-inflammatory drugs presently used CF have several limitations, indicating need for identifying novel molecular targets. To address this issue, we...
Extracts from Nigella arvensis L. seeds, which are widely used as anti-inflammatory remedies in traditional medicine of Northern Africa, were able to inhibit the expression pro-inflammatory neutrophil chemokine Interleukin (IL)-8 Cystic Fibrosis (CF) bronchial epithelial IB3-1 cells exposed Gram-negative bacterium Pseudomonas aeruginosa. The chemical composition extracts led identification three major components, β-sitosterol, stigmasterol and campesterol, most abundant phytosterols,...
Objectives Shwachman-Diamond syndrome (SDS) is a rare autosomal recessive disorder. Its predominant manifestations include exocrine pancreatic insufficiency, bone marrow failure and skeletal abnormalities. Patients frequently present to thrive susceptibility short stature. Average birth weight at the 25th percentile; by first birthday, >50% of patients drop below third percentile for height weight. The study aims estimating growth charts affected SDS in order give reference tool helpful...
Section:ChooseTop of pageAbstract <<MATERIALS AND METHODSRESULTSDISCUSSIONReferencesCITING ARTICLES
One of the clinical features cystic fibrosis (CF) is a deep inflammatory process, which characterized by production and release cytokines chemokines, among interleukin 8 (IL-8) represents one most important. Accordingly, there growing interest in developing therapies against CF to reduce excessive response airways patients. Since transcription factor NF-kappaB plays critical role IL-8 expression, decoy (TFD) strategy might be interest. In order demonstrate that TFD interferes with pathway we...