A5051790707- Advanced Proteomics Techniques and Applications
- Chronic Lymphocytic Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- Monoclonal and Polyclonal Antibodies Research
- Mass Spectrometry Techniques and Applications
- Renal Diseases and Glomerulopathies
- Lymphoma Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Radiopharmaceutical Chemistry and Applications
- Cancer-related gene regulation
- Peptidase Inhibition and Analysis
- Hippo pathway signaling and YAP/TAZ
- Copper-based nanomaterials and applications
- Erythropoietin and Anemia Treatment
- Click Chemistry and Applications
- Blood disorders and treatments
- Periodontal Regeneration and Treatments
- Medical Imaging Techniques and Applications
- Genomics and Rare Diseases
- Erythrocyte Function and Pathophysiology
- Advanced Biosensing Techniques and Applications
- Blood transfusion and management
- Sepsis Diagnosis and Treatment
- Complement system in diseases
- COVID-19 Clinical Research Studies
University of Milano-Bicocca
2017-2024
Mass spectrometry imaging (MSI) is an emerging technology that capable of mapping various biomolecules within their native spatial context, and performing multiomics on formalin-fixed paraffin-embedded (FFPE) tissues may further increase the molecular characterization pathological states. Here we present a novel workflow which enables sequential MSI lipids, N-glycans, tryptic peptides single FFPE tissue section highlight enhanced offered by combining multiple omics data sets. In murine brain...
ABSTRACT Background Multidrug-resistant Pseudomonas aeruginosa (MDRPA) poses significant challenges in hospital settings. Understanding the effects of unprecedented changes brought by COVID-19 pandemic on antimicrobial resistance P. (PA) is essential to inform stewardship efforts. This study investigated dynamics PA bacteremia after start pandemic. Methods single-centre retrospective cohort included adult inpatients with bacteraemia at University Hospital Zurich between January 2014 and...
Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection leads to a wide range of clinical manifestations and determines the need for personalized precision medicine. To better understand biological determinants this heterogeneity, we explored plasma proteome 43 COVID-19 patients with different outcomes by an untargeted liquid chromatography-mass spectrometry approach. The comparison between asymptomatic or pauci-symptomatic subjects (MILDs), hospitalised in oxygen support...
Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease characterized by the aberrant accumulation of extracellular matrix in lungs. nintedanib one two FDA-approved drugs for IPF treatment; however, exact pathophysiological mechanisms progression and response to therapy are still poorly understood. In this work, molecular fingerprint treatment have been investigated mass spectrometry-based bottom-up proteomics paraffin-embedded tissues from bleomycin-induced (BLM) mice. Our results...
The podocyte injury, and consequent proteinuria, that characterize the pathology of idiopathic membranous nephropathy (IMN) is mediated by an autoimmune reaction against antigens. In particular, activation pathways leading to abundant renal deposits complement likely involve binding mannose-binding lectin (MBL) aberrant glycans on immunoglobulins. To obtain a landscape circulatory IgG Fc glycosylation characterizing this disease, we conducted systematic N-glycan profiling study IgG1, 2, 4...
Predicting the prognosis of colorectal cancer (CRC) patients remains challenging and a characterisation tumour immune environment represents one most crucial avenues when attempting to do so. For this reason, molecular approaches which are capable classifying environments associated with infiltrating lymphocytes (TILs) being readily investigated. In proof concept study, we aim explore feasibility using spatial lipidomics by MALDI-MSI distinguish CRC tissue based upon their TIL content....
Noninvasive follicular thyroid neoplasms with papillary-like nuclear features (NIFTP) are low-risk lesions most often characterised by RAS-type mutations. The histological diagnosis may be challenging, and even immunohistochemistry molecular approaches have not yet provided conclusive solutions. This study characterises a set of NIFTPs Matrix-Assisted Laser Desorption/Ionisation (MALDI)-Mass Spectrometry Imaging (MSI) to highlight the proteomic signatures capable overcoming challenges....
The application of innovative spatial proteomics techniques, such as those based upon matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) technology, has the potential to impact research in field nephropathology. Notwithstanding, possibility apply this technology more routine diagnostic contexts remains limited by alternative fixatives employed ultraspecialized field, where most nephropathology laboratories worldwide use bouin-fixed paraffin-embedded (BFPE)...
In the last decade development of new PSMA-ligand based radiopharmaceuticals for imaging and therapy prostate cancer has been a highly active important area research. The most promising derivative in terms interaction with antigen clinical properties found to be "PSMA-617", its lutetium-177 radiolabelled version recently approved by EU USA regulatory agencies therapeutic purposes. For above reasons, derivatives PSMA-617 fluorine-18 may still great interest. This paper proposes comparison two...
Ovarian cancer is a highly lethal gynecological malignancy. Drug resistance rapidly occurs, and different therapeutic approaches are needed. So far, no biomarkers have been discovered to predict early response therapies in the case of multi-treated ovarian patients. The aim our investigation was identify protein panel molecular pathways involved chemotherapy through combination studying proteomics network enrichment analysis by considering subset samples from clinical setting. Differential...
Human platelet lysate (hPL) is considered a valid substitute to fetal bovine serum (FBS) in the expansion of mesenchymal stromal cells (MSC), and it commonly produced starting from intermediate side products whole blood donations. Through freeze–thaw cycles, hPL highly enriched chemokines, growth factors, adhesion immunologic molecules. Cell therapy protocols, using instead FBS for cells, are approved by regulatory authorities without concerns, its administration patients safe. However,...
Idiopathic membranous nephropathy (IMN) is a pathologically defined disorder of the glomerulus, primarily responsible for nephrotic syndromes (NS) in nondiabetic adults. The underlying molecular mechanisms are still not completely clarified. To explore possible and functional signatures, an optimised mass spectrometry (MS) method based on next-generation data-independent acquisition combined with ion-mobility was applied to serum patients affected by IMN (n = 15) or other glomerulopathies...
Since the start of COVID-19 outbreak, more than four million people have died this disease. Given its ability to provide a precise response, mass spectrometry-based proteomics could represent useful tool study pathology. To end, an untargeted nLC-ESI-MS/MS-based method characterise SARS-CoV-2 proteins, including possible variants, and investigate human saliva plasma proteome in single analysis was developed for further application patients. Four recombinant three (S1–S2–RBD) belonging spike...
In the last decade, development of new radiopharmaceuticals for imaging and therapy prostate cancer has been a highly active important area research, especially focusing on prostate-specific membrane antigen (PSMA), an which is upregulated in prostate, as well other tumor cells. A large variety PSMA ligands have radiolabeled, to date. Among various derivatives, PSMA-617 resulted be one most interesting terms interaction with clinical properties, its lutetium-177 labeled version recently...
Anaplastic Large Cell Lymphoma (ALCL) is a rare and aggressive T-cell lymphoma, classified into ALK-positive ALK-negative subtypes, based on the presence of chromosomal translocations involving ALK gene. The current standard treatment for ALCL polychemotherapy, with high overall survival rate. However, subset patients does not respond to or develops resistance these therapies, posing serious challenge clinicians. Recent targeted treatments such as kinase inhibitors anti-CD30 antibody-drug...
Topic: 20. Lymphoma Biology & Translational Research Background: Anaplastic lymphoma kinase (ALK)-positive anaplastic large-cell (ALCL) is an aggressive characterized by expression of the NPM::ALK fusion tyrosine essential for neoplastic cells growth. If disease becomes resistant or relapses after initial chemotherapy, prognosis ALK+ ALCL bleak. Despite being primarily driven constantly active kinase, presence other mutations can influence course and its response to treatment, resulting in...