A5087196327- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Thyroid Cancer Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Renal Diseases and Glomerulopathies
- Molecular Biology Techniques and Applications
- Chronic Kidney Disease and Diabetes
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Peptidase Inhibition and Analysis
- Astrophysics and Cosmic Phenomena
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Cells and Metastasis
- Prostate Cancer Treatment and Research
- Glycosylation and Glycoproteins Research
- Lysosomal Storage Disorders Research
- Renal cell carcinoma treatment
- Platelet Disorders and Treatments
- Vasculitis and related conditions
- Tumors and Oncological Cases
- Head and Neck Anomalies
- Glioma Diagnosis and Treatment
- Renal and related cancers
- Advanced Glycation End Products research
- Gamma-ray bursts and supernovae
- X-ray Spectroscopy and Fluorescence Analysis
University of Milano-Bicocca
2016-2025
University of Manchester
2023
Purdue University West Lafayette
2016-2022
University of Leicester
2020
Mount Sinai Medical Center
2019
John Radcliffe Hospital
2018
University of Oxford
2018
University of Illinois Urbana-Champaign
2017
LAC+USC Medical Center
2017
Center for Cancer Research
2016
The paper shows a new method for the application of Matrix Assisted Laser Desorption/Ionisation (MALDI) Mass Spectrometry Imaging (MSI) technology on formalin-fixed paraffin-embedded (FFPE) tissue samples.
Therapeutic targeting of late-stage breast cancer is limited by an inadequate understanding how tumor cell signaling evolves during metastatic progression and the currently available small molecule inhibitors capable these processes. Herein, we demonstrate that both β3 integrin fibroblast growth factor receptor-1 (FGFR1) are part epithelial-mesenchymal transition (EMT) program required to facilitate outgrowth in response factor-2 (FGF2). Mechanistically, physically disrupts interaction...
Mass spectrometry imaging (MSI) is an emerging technology that capable of mapping various biomolecules within their native spatial context, and performing multiomics on formalin-fixed paraffin-embedded (FFPE) tissues may further increase the molecular characterization pathological states. Here we present a novel workflow which enables sequential MSI lipids, N-glycans, tryptic peptides single FFPE tissue section highlight enhanced offered by combining multiple omics data sets. In murine brain...
MALDI-HiPLEX-IHC mass spectrometry imaging (MSI) represents a newly established workflow to map tens of antibodies linked photocleavable tags (PC-MTs), which report the distribution antigens in formalin-fixed paraffin-embedded (FFPE) tissue sections. While this highly multiplexed approach has previously been integrated with untargeted methods, possibility mapping target cell and performing bottom-up spatial proteomics on same section yet be explored. This proof-of-concept study presents...
Abstract Background The αvβ6- and αvβ8-integrins, two cell-adhesion receptors upregulated in many solid tumors, can promote the activation of transforming growth factor-β (TGFβ), a potent immunosuppressive cytokine, by interacting with RGD sequence latency-associated peptide (LAP)/TGFβ complex. We have previously described chromogranin A-derived peptide, called “peptide 5a ”, which recognizes RGD-binding site both αvβ6 αvβ8 high affinity selectivity, efficiently accumulates or αvβ8-positive...
The accurate and rapid identification of bacterial pathogens poses a significant challenge in clinical diagnostics, environmental monitoring, microbial research. Lipidomics proteomics serve as powerful methodologies for characterization; however, the complexity biological matrices low abundance lipids often limit effective detection. This study introduces graphene-polyglycerol amine (G-PGA) novel nanomaterial that enhances selective trapping detection Escherichia coli (E. coli) using...
Aims The Oxford Classification E score (endocapillary hypercellularity) predicts renal functional decline in IgA nephropathy (Ig AN ) patients free from steroid/immunosuppressive ( IS therapy, but is poorly reproducible. We hypothesise that endocapillary hypercellularity reflects glomerular inflammation and the presence of CD 68‐positive cells a more robust marker score. Methods results were quantified glomeruli tubulointerstitium biopsies 118 Ig patients, cell counts correlated with...
Idiopathic glomerulonephritis (GN), such as membranous glomerulonephritis, focal segmental glomerulosclerosis (FSGS), and IgA nephropathy (IgAN), represent the most frequent primary glomerular kidney diseases (GKDs) worldwide. Although renal biopsy currently remains gold standard for routine diagnosis of idiopathic GN, invasiveness diagnostic difficulty related with this procedure highlight strong need new prognostic biomarkers to be translated into less invasive tools. MALDI-MS imaging...
Formalin-fixed paraffin-embedded (FFPE) tissue represents the primary source of clinical and is routinely used in MALDI-MSI studies. However, it not particularly suitable for lipidomics imaging given that many species are depleted during processing. Irrespective, a number solvent-resistant lipids remain, but their extraction may be hindered by cross-link between proteins. Therefore, an antigen retrieval step could enable greater provide information complementary to which can obtained from...
In this contribution, we report an efficient approach to multiplex electrospray ionization (ESI) sources for applications in analytical and preparative mass spectrometry. This is achieved using up four orthogonal injection inlets implemented on the opposite sides of electrodynamic ion funnel interface. We demonstrate that both total current transmitted through spectrometer signal-to-noise ratio increase by 3.8-fold compared one inlet. The performance new multiplexing was examined different...
In cancer microenvironment, aberrant glycosylation events of ECM proteins and cell surface receptors occur. We developed a protocol to generate 3D bioprinted models colorectal (CRC) crosslinking hyaluronic acid gelatin functionalized with three signalling glycans characterized in CRC, 3'-Sialylgalactose, 6'-Sialylgalactose 2'-Fucosylgalactose. The crosslinking, performed exploiting azide 4arm-PEG-dibenzocyclooctyne, resulted biocompatible hydrogels that were commercial CRC cells HT-29...
Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease characterized by the aberrant accumulation of extracellular matrix in lungs. nintedanib one two FDA-approved drugs for IPF treatment; however, exact pathophysiological mechanisms progression and response to therapy are still poorly understood. In this work, molecular fingerprint treatment have been investigated mass spectrometry-based bottom-up proteomics paraffin-embedded tissues from bleomycin-induced (BLM) mice. Our results...
Purpose Urinary extracellular vesicles (UEVs) are a novel source for disease biomarker discovery. However, Tamm–Horsfall protein (THP) is still challenge proteomic analysis since it can inhibit detection of low‐abundance proteins. Here, we introduce new approach that does not involve an ultracentrifugation step to enrich and reduces the amount THP manageable levels. Experimental design UEVs were dialyzed ultrafiltered after reduction alkylation. The retained fraction was digested with...
Several promising biomarkers have been found for RCC, but none of them has used in clinical practice predicting tumour progression. The most widely features aggressiveness still remain the cancer stage, size and grade. Therefore, aim our study is to investigate urinary peptidome search identify peptides whose concentrations urine are linked growth measure data. A proteomic approach applied ccRCC (n = 117) based on prefractionation with activated magnetic beads followed by MALDI-TOF profiling...
The glomerulocentric International Society of Nephrology/Renal Pathology (ISN/RPS) classification is the gold standard for evaluation lupus nephritis, while tubulointerstitial (TIN) parameters are often under-recognized in pathological reports.Renal biopsies from 142 patients who underwent repeat biopsy (RB) were evaluated following histological parameters: (i) inflammatory interstitial infiltrates; (ii) fibrosis; (iii) tubulitis; and (iv) tubular atrophy. inter-relationships between four...
Several proteomic strategies are used extensively for the purpose of biomarker discovery and in order to obtain insights into molecular aspects cancers, using either body fluids or tissue as samples. Among them, MALDI‐imaging can be applied cytological thyroid specimens investigate signatures different pathological conditions highlight differences proteome that relevance diagnostic pathogenetic research. In this study, 26 ex‐vivo fine needle aspirations from benign nodules ( n = 13)...
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) technology has advanced rapidly during recent years with the development of instruments equipped low-diameter lasers that are suitable for high spatial resolution imaging. This may provide significant advantages in certain fields molecular pathology where more specific protein fingerprints individual cell types required, such as renal pathology.Here MALDI-MSI analysis a cohort membranous nephropathy (MN)...
Objective Matrix‐assisted laser desorption/ionization ( MALDI ) imaging mass spectrometry IMS is a unique proteomic technology that explores the spatial distribution of biomolecules directly in situ , thus integrating molecular and morphological information. The possibility correlating maps multiple analyses with cytological features makes it an ideal research tool for discovering new diagnostic markers. A previous study showed ‐ could help discrimination between different types thyroid...
Renal Cell Carcinoma (RCC) is typically asymptomatic and surgery usually increases patient's lifespan only for early stage tumours. Moreover, solid renal masses cannot be confidently differentiated from RCC. Therefore, markers to distinguish malignant kidney tumours their detection are needed. Two different peptide signatures were obtained by a MALDI-TOF profiling approach based on urine pre-purification C8 magnetic beads. One cluster of 12 signals could differentiate (n = 137) benign...