A5083292241- Renal Diseases and Glomerulopathies
- Extracellular vesicles in disease
- Chronic Kidney Disease and Diabetes
- Renal and related cancers
- Ion Transport and Channel Regulation
- Biomedical Research and Pathophysiology
- Glycosylation and Glycoproteins Research
- MicroRNA in disease regulation
- Escherichia coli research studies
- Dialysis and Renal Disease Management
- Genetic and Kidney Cyst Diseases
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Liver Disease Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Pancreatic function and diabetes
- Birth, Development, and Health
- Viral Infections and Vectors
- Monoclonal and Polyclonal Antibodies Research
- Renal cell carcinoma treatment
- Erythrocyte Function and Pathophysiology
- Galectins and Cancer Biology
- Toxin Mechanisms and Immunotoxins
- Complement system in diseases
- Wnt/β-catenin signaling in development and cancer
- Tuberous Sclerosis Complex Research
University of Helsinki
2010-2023
Institute for Molecular Medicine Finland
2006-2023
University Medical Center Hamburg-Eppendorf
2018-2023
Universität Hamburg
2018-2023
Finland University
2021
Dublin City University
2009-2019
University of Freiburg
2017
University Medical Center Freiburg
2016
Third Affiliated Hospital of Southern Medical University
2013
Folkhälsans Forskningscentrum
2011
Abstract Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast‐growing scientific field. In the last decade urinary (uEVs) were shown to mirror molecular processes as well physiological pathological conditions kidney, urothelial prostate tissue. Therefore, several methods isolate characterize uEVs have been developed. However, methodological aspects EV separation analysis, including normalization...
The mechanisms underpinning the regenerative capabilities of mesenchymal stem cells (MSC) were originally thought to reside in their ability recognise damaged tissue and differentiate into specific cell types that would replace defective cells. However, recent work has shown molecules produced by MSCs (secretome), particularly those packaged extracellular vesicles (EVs), rather than themselves are responsible for repair.Here we have a secretome from adipose-derived (ADSC) is free exogenous...
Some skin and soft tumors, which generally are assumed to be derived from endothelial cells or blood vessels, were characterized with fluorochrome-labeled Ulex europaeus I agglutinin (UEA I), recently shown bind specifically in various normal human tissues. The staining pattern was compared that obtained immunostaining using antibodies against factor-VIII-related antigen (FVIII-RAG), a known marker for cells. results showed UEA-I is specific more sensitive the benign vascular lesions as...
Six fluorochrome-coupled lectins with different sugar specificities were used to stain frozen tissue sections of kidneys from 14 animal species including mammals, avians, reptiles, and fresh water fish. Each lectin seemed have a species-, but not strain-, specific binding pattern. Some lectins, however, bound the same parts nephron in all animals studied. Wheat germ agglutinin (WGA) prominently glomeruli kidneys. Dolichos biflorus (DBA) bind only group distal tubules most animals, whereas...
Diabetic kidney disease (DKD) is a devastating complication that affects an estimated third of patients with type 1 diabetes mellitus (DM). There no cure once the diagnosed, but early treatment at sub-clinical stage can prevent or least halt progression. DKD clinically diagnosed as abnormally high urinary albumin excretion rate (AER). We hypothesize subtle changes in urine metabolome precede significant rise AER. To test this, 52 diabetic were recruited by FinnDiane study had normal AER...
Urinary extracellular vesicles provide a novel source for valuable biomarkers kidney and urogenital diseases: Current isolation protocols include laborious, sequential centrifugation steps which hampers their widespread research clinical use. Furthermore, large individual urine sample volumes or sizable target cohorts are to be processed (e.g. biobanking), the storage capacity is an additional problem. Thus, alternative methods necessary overcome such limitations. We have developed practical...
ABSTRACT The utilization of extracellular vesicles (EVs) in clinical theranostics has rapidly advanced the past decade. In November 2018, International Society for Extracellular Vesicles (ISEV) held a workshop on “EVs Clinical Theranostic”. Here, we report conclusions roundtable discussions current advancement analysis technologies and provide some guidelines to researchers field consider use EVs application. main challenges requirements EV separation characterization strategies, quality...
Dual-laser flow cytometry, based on the properties of DNA-binding dyes Hoechst 33342 and propidium iodide, was used, with light electron microscopy DNA fragmentation studies, to define influence lipoxygenase-derived eicosanoids apoptosis human polymorphonuclear neutrophils (PMN) in vitro. Apoptosis characterized by progression through an early apoptotic phase condensation chromatin coalescence nuclear lobes, a late degradation evanescence, secondary necrosis. Prolonged exposure PMN...
Diabetic nephropathy is the leading cause of end-stage renal disease. Because early diagnosis and treatment may prevent complication, new tools for an detection are needed. One key components glomerular filtration slit spanning between neighboring podocytes nephrin. Its expression altered in experimental models diabetes also various human proteinuric diseases, including diabetes. We studied whether type 1 diabetic patients with or without exhibit immunoreactive nephrin urine, reflecting...
We studied domains in the human bladder that acted as receptors for Escherichia coli P, S, type 1, 1C, and O75X fimbriae or adhesin kidneys were E. 1C fimbriae. Binding sites frozen tissue sections localized by direct staining with fluorochrome-labeled recombinant strains indirect immunofluorescence purified adhesins. In bladder, P S showed closely similar binding to epithelial muscular layers, also bound connective elements. Type 1 vascular walls muscle cells, whereas avidly elements some...
Purified S fimbriae and an Escherichia coli strain carrying the recombinant plasmid pANN801-4 that encodes were tested for adhesion to frozen sections of human kidney. The bacteria bound same tissue domains, in both cases binding was specifically inhibited by receptor analog fimbria, sialyl(alpha 2-3)lactose. epithelial elements kidneys; cells proximal distal tubules as well collecting ducts visceral parietal glomerular epithelium. In addition, they vascular endothelium glomeruli renal...
Although metabolic derangement plays a central role in diabetic nephropathy, better understanding of secondary mediators injury may lead to new therapeutic strategies. Expression macrophage migration inhibitory factor (MIF) is increased experimental and tubulointerstitial mRNA expression its receptor, CD74, has been observed human nephropathy. Whether CD74 transduces MIF signals podocytes, however, unknown. Here, we found glomerular be Pima Indians with type 2 diabetes Immunohistochemistry...
Urinary exosomes represent a precious source of potential biomarkers for disease biology. Currently, the methods vesicle isolation are severely restricted by tendency entrapment, e.g. abundant Tamm-Horsfall protein (THP) polymers. Treatment reducing agents such as dithiothreitol (DTT) releases entrapped vesicles, thus increasing final yield. However, this harsh treatment can cause remodelling all those proteins which feature extra-vesicular domains stabilized internal disulfide bridges and...
Epithelial cells lining the urinary tract secrete exosomes (40–100 nm) that can be targeted to specific modulating their functionality. One potential targeting mechanism is adhesion between vesicle surface glycoproteins and target cells. This makes glycopeptide analysis of important. Exosomes reflect physiological state parent cells; therefore, they are a good source biomarkers for urological other diseases. Moreover, urine collection easy noninvasive give information about renal systemic...