A5024263446- Prostate Cancer Treatment and Research
- Salivary Gland Tumors Diagnosis and Treatment
- Bladder and Urothelial Cancer Treatments
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Prostate Cancer Diagnosis and Treatment
- Renal cell carcinoma treatment
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- Ear and Head Tumors
- Cancer, Lipids, and Metabolism
- Cancer-related gene regulation
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Carcinogens and Genotoxicity Assessment
- Tumors and Oncological Cases
- DNA Repair Mechanisms
- Urinary and Genital Oncology Studies
- Cancer Cells and Metastasis
- CRISPR and Genetic Engineering
- Oral and Maxillofacial Pathology
- Cancer, Hypoxia, and Metabolism
- Cancer and Skin Lesions
Radboud University Nijmegen
2015-2024
Radboud University Medical Center
2015-2024
University Medical Center
2018-2024
Radboud Institute for Molecular Life Sciences
2015-2024
Utrecht University
2012
University Medical Center Utrecht
2012
Centre international de recherche sur le cancer
1997-2002
Leiden University
1993-1995
Abstract Purpose: Early detection of prostate cancer can increase the curative success rate for cancer. We studied diagnostic usefulness TMPRSS2-ERG fusion transcripts as well combination antigen 3 (PCA3) RNA and in urinary sediments after digital rectal examination (DRE). Experimental Design: A total 78 men with cancer–positive biopsies 30 cancer–negative were included this study. After DRE, first voided urine was collected, obtained. used semiquantitative reverse transcription-PCR (RT-PCR)...
Abstract Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast‐growing scientific field. In the last decade urinary (uEVs) were shown to mirror molecular processes as well physiological pathological conditions kidney, urothelial prostate tissue. Therefore, several methods isolate characterize uEVs have been developed. However, methodological aspects EV separation analysis, including normalization...
Men with germline breast cancer 1, early onset (BRCA1) or 2, (BRCA2) gene mutations have a higher risk of developing prostate (PCa) than noncarriers. IMPACT (Identification genetic predisposition to ProstAte Cancer: Targeted screening in BRCA1/2 mutation carriers and controls) is an international consortium 62 centres 20 countries evaluating the use targeted PCa men mutations.
Abstract Translocations fusing the strong androgen-responsive gene, TMPRSS2, with ERG or other oncogenic ETS factors may facilitate prostate cancer development. Here, we studied 18 advanced cancers for factor alterations, using reverse transcription-PCR and DNA RNA array technologies, identified putative downstream gene targets from microarray data of 410 samples. Out 27 factors, was most frequently overexpressed. Seven cases showed TMPRSS2:ERG fusions, whereas TMPRSS2:ETV4 fusion seen in...
The presence of the retrovirus xenotropic murine leukaemia virus-related virus (XMRV) has been reported in peripheral blood mononuclear cells patients with chronic fatigue syndrome. Considering potentially great medical and social relevance such a discovery, we investigated whether this finding could be confirmed an independent European cohort syndrome.Analysis well defined matched neighbourhood controls by polymerase chain reaction.Certified (ISO 15189) laboratory clinical virology...
Abstract PURPOSE PCA3 urine tests have shown to improve the specificity in prostate cancer (PCa) diagnosis, and thus potential reduce number of unnecessary biopsies predict repeat biopsy outcomes. In this study, was correlated with clinical stage, Gleason score (GS), radical prostatectomy GS, tumor volume, pathological stage assess its as predictor PCa aggressiveness. METHODS 351 men admitted for based on serum PSA levels >3 ng/ml, an abnormal DRE, and/or a family history were included....
European genome-wide association study identifies SLC14A1 as a new urinary bladder cancer susceptibility gene.
Mutations in the p53 tumor suppressor gene frequently fall within specific DNA-binding domain and prevent molecule from transactivating normal targets. activity is regulated vitro by metal ions redox conditions, but whether these factors also regulate vivo unclear. To address this question, we have analyzed effect of pyrrolidine dithiocarbamate (PDTC) on cell lines expressing wild-type p53. PDTC commonly regarded as an antioxidant, it can bind transport external copper into cells thus exert...
Current endocrine treatment for advanced prostate cancer does not result in a complete ablation of adrenal androgens. Adrenal androgens can be metabolized by cells, which is one the mechanisms associated with progression to castration-resistant (CRPC). Aldo-keto reductase family 1 member C3 (AKR1C3) steroidogenic enzyme that plays crucial role conversion androgen dehydroepiandrosterone (DHEA) into high-affinity ligands receptor (testosterone [T] and dihydrotestosterone [DHT]). The aim this...
Rationale: Treatment options for recurrent and/or metastatic (R/M) adenoid cystic carcinoma (ACC) and salivary duct (SDC), major subtypes of gland cancer, are limited.Both tumors often show overexpression prostate-specific membrane antigen (PSMA).In prostate PSMA-ligands labeled with 68 Ga or 177 Lu used imaging therapy, respectively.Primary aim this study in R/M ACC SDC patients was to systematically investigate Ga-PSMA-uptake by PET/CT determine if PSMA radionuclide therapy could be a...
Hyperthermia combined with chemotherapy is not a novel cancer treatment. However, the working mechanism of this combination therapy fully understood. In current in vitro study we investigated differences cytotoxicity 4 chemotherapeutic agents at 37C or 43C.The human transitional cell carcinoma lines used were RT4, RT112, 253J and T24. Cells seeded 96-well microtiter plates. After 24 hours cells treated for 60 minutes increasing concentrations mitomycin C, epirubicin, gemcitabine EO9...
The transcription factor p53 controls the proliferation and survival of cells exposed to DNA damage. specific DNA-binding domain (residues 102–292) has a complex tertiary structure that is stabilized by zinc. In this study, we showed exposure cultured membrane-permeable chelator N,N,N′, N′-tetrakis(2- pyridylmethyl)ethylenediamine induced wild-type accumulate in an immunologically "mutant" form (Pab240+, Pab1620–) with decreased activity. Removal...
Reversing epithelial-to-mesenchymal transition (EMT) in cancer cells has been widely considered as an approach to combat progression and therapeutic resistance, but a limited number of broadly comprehensive investigations miRNAs involved this process have conducted. In study, we screened library 1120 miRNA for their ability transcriptionally activate the E-cadherin gene CDH1 promoter reporter assay measure EMT reversal. By approach, defined miR-520f novel EMT-reversing miRNA. expression was...