A5026766359- Renal Diseases and Glomerulopathies
- Dialysis and Renal Disease Management
- Chronic Kidney Disease and Diabetes
- Renal Transplantation Outcomes and Treatments
- Bacterial Identification and Susceptibility Testing
- Systemic Lupus Erythematosus Research
- Polyomavirus and related diseases
- Cytomegalovirus and herpesvirus research
- Renal and Vascular Pathologies
- Vasculitis and related conditions
- Diabetes Treatment and Management
- Organ Transplantation Techniques and Outcomes
- Renal and related cancers
- Central Venous Catheters and Hemodialysis
- Organ Donation and Transplantation
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Pleural and Pulmonary Diseases
- Renal cell carcinoma treatment
- Immune Cell Function and Interaction
- Parathyroid Disorders and Treatments
- Antibiotic Resistance in Bacteria
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Pediatric Urology and Nephrology Studies
- T-cell and B-cell Immunology
- Patient-Provider Communication in Healthcare
Sir Charles Gairdner Hospital
2016-2025
The University of Western Australia
2016-2025
Harry Perkins Institute of Medical Research
2014-2025
Curtin University
2025
Royal Prince Alfred Hospital
2024
Sir Charles Gairdner Osborne Park Health Care Group
2024
Queen Elizabeth II Medical Centre
2014-2023
Pathwest Laboratory Medicine
2023
Melbourne Genomics Health Alliance
2019
Royal Perth Hospital
1998-2019
In the 330-patient ADVOCATE trial of avacopan for treatment antineutrophil cytoplasmic autoantibody (ANCA)-associated vasculitis, in which 81% patients had renal involvement, estimated glomerular filtration rate (eGFR) increased on average 7.3 ml/min per 1.73 m2 group and 4.1 prednisone (P = 0.029) at week 52. This new analysis examines results patient subgroup with severe insufficiency enrollment into trial, i.e., eGFR ≤20 m2.eGFR was determined baseline over course trial. Changes were...
Diagnostic genomic sequencing is the emerging standard of care in nephrology. There a growing need to scale up implementation diagnostics nationally improve patient outcomes.
The Duffy antigen/receptor for chemokines (DARC) is an unusual chemokine receptor that binds a large number of inflammatory both the CC and CXC families with nanomolar affinity, yet it lacks ability to signal upon ligand binding. Using bioluminescent resonant energy transfer, we have demonstrated first time DARC exists as constitutive homo-oligomer in living cells furthermore hetero-oligomerizes CCR5. DARC-CCR5 interaction impairs chemotaxis calcium flux through CCR5, whereas internalization...
Despite an increasing awareness of the importance innate immunity, roles natural killer (NK) cells in transplant rejection and antiviral cancer immunity during immunosuppression have not been clearly defined.To address this issue we developed a quantitative assay NK cell function that can be used on clinical samples studied influence function. degranulation intracellular interferon (IFN)-γ production were determined by flow cytometry peripheral blood samples.Overnight ex vivo treatment from...
Cytomegalovirus (CMV) establishes a lifelong infection that is efficiently controlled by the immune system; this can be reactivated in case of immunosuppression such as following solid organ transplantation. CMV viraemia has been associated with disease, well increased mortality and allograft failure. Prophylactic antiviral medication routinely given to renal transplant recipients, but reactivation during cessation prophylaxis known occur. The aims study were assess incidence, timing impact...
The platelet receptor CLEC-2 binds to the snake venom toxin rhodocytin and tumor cell surface protein podoplanin. Binding of either these ligands promotes phosphorylation a single tyrosine residue in YXXL motif intracellular domain CLEC-2. Phosphorylation this initiates binding spleen kinase (Syk) triggers further downstream signaling events ultimately potent activation aggregation. However, it is unclear how can interact efficiently with Syk, which usually recognizes two tandem repeats...
The expanding global distribution of multi-resistant Klebsiella pneumoniae demands faster antimicrobial susceptibility testing (AST) to guide antibiotic treatment. Current ASTs rely on time-consuming differentiation resistance and after initial isolation bacteria from a clinical specimen. Here we describe flow cytometry workflow determine carbapenem bacterial cell characteristics in an international K. isolate collection (n = 48), with range carbapenemases. Our cytometry-assisted test (FAST)...
Pleural infection is common. Evacuation of infected pleural fluid essential for successful treatment, but it often difficult because adhesions/loculations within the effusion and viscosity fluid. Intrapleural delivery tissue plasminogen activator (tPA) (to break adhesions) deoxyribonuclease (DNase) reduce viscosity) has recently been shown to improve clinical outcomes in a large randomized study infection. Clinical studies intrapleural fibrinolytic therapy have consistently subsequent...
Aims The Oxford Classification E score (endocapillary hypercellularity) predicts renal functional decline in IgA nephropathy (Ig AN ) patients free from steroid/immunosuppressive ( IS therapy, but is poorly reproducible. We hypothesise that endocapillary hypercellularity reflects glomerular inflammation and the presence of CD 68‐positive cells a more robust marker score. Methods results were quantified glomeruli tubulointerstitium biopsies 118 Ig patients, cell counts correlated with...
To assess the prevalence and trends of chronic kidney disease (CKD) in Western Australia (WA) from 2010 to 2020 using linked pathology data. A retrospective observational cohort study de-identified data WA providers, hospital morbidity records mortality records. Australian population-based study. All individuals aged 18 years older with at least one serum creatinine test. CKD status as determined by estimated glomerular filtration rate urine albumin-creatinine ratio. Analysing 2 501 188...
Genetic kidney disease (GKD) significantly affects the community and is responsible for a notable portion of adult cases about half in paediatric patients. It substantially impacts quality life expectancy affected children adults across all stages disease. Precise genetic diagnosis GKD promises to improve patient outcomes, provide access targeted treatments, reduce burden individuals, families, healthcare systems. investigations are increasingly used nephrology practice; however, many...
There remains a lack of evidence based guidelines regarding the benefit applying scoring systems in outpatient nephrology setting. As such, we set out to investigate and follow-up utility Clinical Frailty Scale (CFS) for ongoing management patients with end stage kidney disease. To explore association between frailty, as measured by Score 5-8, long-term outcomes cohort chronic disease (CKD). This was 3-year analysis prospective study. study included participants age >50 years CKD an...
Abstract Purpose The urinary albumin:creatinine ratio (uACR) can exhibit significant temporal changes but few studies have characterized transition patterns between uACR categories in type 2 diabetes. study aim was to use group-based trajectory modelling (GBTM) identify clusters of people with diabetes and distinct trajectories. Participants methods Of 1482 participants the observational Fremantle Diabetes Study Phase II, 1145 (77.3%; mean age 65.4 years, 53.3% males) ≥2 biennial...
Summary Infection with the polyoma virus BK (BKV) is a major cause of morbidity following renal transplantation. Limited understanding anti-viral immune response has prevented design strategy that balances treatment preservation graft function. The proven utility interferon-gamma enzyme-linked immunospot (ELISPOT) assays to measure T cell responses in immunocompetent hosts was basis for trying develop rational approach management BKV In sample transplant recipients and healthy controls,...
Reactivation of polyomavirus BK (BKV) after renal transplantation can lead to allograft dysfunction or loss with early detection improving outcomes. Current guidelines recommend quantitative polymerase chain reaction for surveillance; however, urinary decoy cell is a potentially cost-effective alternative. We present the outcomes from an intensive BKV surveillance program using initial screening starting 2 weeks transplantation.Records all recipients kidney (n=211) simultaneous and pancreas...
Rheumatoid arthritis (RA) is a systemic autoimmune disease primarily affecting synovial joints in which the development of autoantibodies represents failure normal tolerance mechanisms, suggesting role for follicular helper T cells (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msub><mml:mtext>T</mml:mtext><mml:mrow><mml:mtext>FH</mml:mtext></mml:mrow></mml:msub></mml:mrow></mml:math>) genesis autoimmunity. To determine whether quantitative or qualitative...
Membranous glomerulonephritis (MGN) is the commonest cause of nephrotic syndrome in adults.1,2 The majority cases are idiopathic (primary MGN), however ∼30% secondary to systemic diseases such as SLE, epithelial malignancies, medications or exposure heavy metals.3 Recently, an autoantibody has been identified >70% MGN with specificity for M-type phospholipase A2 receptor (PLA2R).4 Skin-lightening creams, which may contain mercury salts, widely used ethnic subgroups where paler skin seen a...
The annual mortality burden of antimicrobial resistant infections exceeds 1.27 million/year. With serious infections, every hour without effective therapy results in a 6.7% increased risk death. New technology that delivers actionable pathology clinically-relevant timeframes is an urgent priority. We present the development and validation acoustic-enhanced flow cytometric (AFC) workflow provides same-day confirmation infection susceptibility, using peritoneal dialysis (PD)-associated...