A5012017299- Blood Coagulation and Thrombosis Mechanisms
- Dialysis and Renal Disease Management
- Chronic Kidney Disease and Diabetes
- Acute Kidney Injury Research
- Renal Diseases and Glomerulopathies
- Central Venous Catheters and Hemodialysis
- Protease and Inhibitor Mechanisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Venous Thromboembolism Diagnosis and Management
- Hemophilia Treatment and Research
- Cell Adhesion Molecules Research
- Health disparities and outcomes
- Health Systems, Economic Evaluations, Quality of Life
- Immune Response and Inflammation
- Platelet Disorders and Treatments
- Cardiac Fibrosis and Remodeling
- Organ Transplantation Techniques and Outcomes
- Vasculitis and related conditions
- Renal and Vascular Pathologies
- Pharmaceutical Practices and Patient Outcomes
- Pregnancy and preeclampsia studies
- Inflammation biomarkers and pathways
- Sepsis Diagnosis and Treatment
- Antiplatelet Therapy and Cardiovascular Diseases
- Electrolyte and hormonal disorders
UNSW Sydney
2013-2024
Prince of Wales Hospital
2010-2024
Leicester General Hospital
2023
University of Leicester
2023
Kidney Health Australia
2020
Scripps Research Institute
1998-2005
University of Michigan–Ann Arbor
2005
University of Missouri
2005
Harvard University
2005
Mount Sinai Medical Center
2002
Recognition of bacterial endotoxin (LPS) elicits multiple host responses, including activation cells the innate immune system. LPS exposure occurs repeatedly during septicemia, making strict regulation gene expression necessary. Such might prevent, for example, continuous production proinflammatory cytokines such as tumor necrosis factor (TNF), which could lead to severe vascular collapse. Tolerance is characterized by a diminished TNF prolonged LPS, and therefore likely represent an...
Tissue factor (TF) expression is associated with life-threatening thrombosis in a variety of human diseases, including sepsis, cancer, and atherosclerosis. Recently, it was shown that inactivation the murine TF (mTF) gene results embryonic lethality. To date, despite extensive studies on regulation promoter vitro, no have examined cis-acting regulatory elements control vivo. Here we report (hTF) minigene containing cDNA directed low level (approximately 1% relative to mouse TF) both...
Abstract Pancreatic cancer patients have a high risk of venous thromboembolism (VTE). Plasminogen activator inhibitor 1 (PAI-1) inhibits plasminogen activators and increases the thrombosis. PAI-1 is expressed by pancreatic tumors human cell lines. However, to date, there are no studies analyzing association active VTE in patients. We investigated plasma In addition, we determined if presence expressing impairs thrombus resolution mice. Plasma levels with mice bearing were enzyme-linked...
We employed a novel mouse line that expresses low levels of human tissue factor (TF) in the absence murine TF to analyze role gestation. Low-TF female mice had 14–18% incidence fatal postpartum uterine hemorrhage, suggesting plays an important hemostasis. mated with low-TF male 42% midgestational hemorrhage ( n = 41), whereas no hemorrhages were observed wild-type 43). Placentas embryos from both and normal (+/−) females abnormal contained numerous maternal blood pools labyrinth. null...
Exposure of blood to tissue factor (TF) activates the extrinsic (TF:FVIIa) and intrinsic (FVIIIa:FIXa) pathways coagulation. In this study, we found that mice expressing low levels human TF (≈1% wild-type levels) in an mTF −/− background had significantly shorter lifespans than mice, part, because spontaneous fatal hemorrhages. All low-TF exhibited a selective heart defect consisted hemosiderin deposition fibrosis. Direct intracardiac measurement demonstrated 30% reduction ( P < 0.001)...
Along with diabetes and obesity, chronic kidney disease (CKD) is increasing across the globe. Although some data support an effect of maternal obesity on offspring kidney, impact paternal unknown; thus, we have studied prior to conception. Male Sprague Dawley rats were fed chow diet or high fat (HFD) for 13–14 weeks before mating chow-fed females. weaned onto killed at 27 renal gene expression histology. Fathers HFD 30% heavier than Controls mating. At age obese fathers weighed 10% less;...
ABSTRACT Tissue factor (TF) is the major activator of coagulation protease cascade and contributes to lethality in sepsis. Despite several studies analyzing TF expression animal models endotoxemia, there remains debate about cell types that are induced express different tissues. In this study, we performed a detailed analysis induction mRNA protein two rabbit endotoxemia better understand may contribute local fibrin deposition disseminated intravascular coagulation. Northern blot...
After oral administration of cysts the intestinal protozoan parasite, Giardia muris, young male C3H/He mice are chronically infected, whereas BALB/c demonstrate a rapidly resolving pattern infection. Both strains injected with trophozoites in adjuvant and challenged orally develop serum antibodies to numerous trophozoite proteins. A limited number these protein antigens was differentially immunoprecipitated by sera from resistant susceptible exposed G. muris. 35S-methionine-labelled better...
Summary High circulating levels of the procoagulant molecule tissue factor (TF) are associated with thrombosis in a variety diseases including unstable angina, cancer, and sepsis. Currently, there no clinical assays to measure level TF activity whole blood. We present an assay called Tissue Factor Clotting Time (“TiFaCT™”) that detects fibrin formation human The mean baseline clotting time healthy population was 472 ± 94 s (mean SD, n = 150). Bacterial lipopolysaccharide (LPS or endotoxin)...
In kidney disease, the concept of loss is widely discussed but minimally researched. It appears that dialysis patients who grieve a range losses suffer increased depression and reduced quality life (QoL). Limited research partly due to lack relevant measure. The study presented here developed measure tested criterion validity in relation QoL.In cross-sectional observational study, 151 long-term were interviewed using standardized psychometric measures Kidney Disease Loss Scale (KDLS), for...
Early recognition of hospital-acquired acute kidney injury (AKI) may improve patient management and outcomes.This multicentre study was conducted at three hospitals (H1-intervention; H2 H3-controls) served by a single laboratory. The intervention bundle [an interruptive automated alerts (aAlerts) showing AKI stage baseline creatinine in the eMR, guide junior medical staff education] implemented only H1. Outcome variables included length-of-stay (LOS), all-cause in-hospital mortality...
Background We recently demonstrated that TNF-related apoptosis-inducing ligand (TRAIL) is protective of diet-induced diabetes in mice. While TRAIL has been implicated chronic kidney disease, its role vivo diabetic nephropathy not clear. The present study investigated the pathogenesis using TRAIL-/-ApoE-/- Methods and ApoE-/- mice were fed a high fat diet for 20 w. Plasma glucose insulin levels assessed over 0, 5, 8 At w, markers function including creatinine, phosphate, calcium cystatin C...