A5031619803- Atherosclerosis and Cardiovascular Diseases
- Angiogenesis and VEGF in Cancer
- Cell death mechanisms and regulation
- Cell Adhesion Molecules Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cholesterol and Lipid Metabolism
- Cancer, Lipids, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Chemokine receptors and signaling
- MicroRNA in disease regulation
- Peripheral Artery Disease Management
- Immunotherapy and Immune Responses
- Lipoproteins and Cardiovascular Health
- Drug Transport and Resistance Mechanisms
- Cancer-related molecular mechanisms research
- Caveolin-1 and cellular processes
- Biomarkers in Disease Mechanisms
- Lipid metabolism and disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Liver Disease Diagnosis and Treatment
- Signaling Pathways in Disease
- Pluripotent Stem Cells Research
- Acute Ischemic Stroke Management
- Selenium in Biological Systems
- CAR-T cell therapy research
The Heart Research Institute
2016-2025
The University of Sydney
2016-2025
University of Kentucky
2023
UNSW Sydney
2007-2021
Anzac Research Institute
2005-2015
Concord Repatriation General Hospital
2005-2015
Inserm
2015
Sorbonne Université
2015
Centre for Human Drug Research
2015
Scripps Research Institute
2015
To study the acceptor specificity for human ABCG1 (hABCG1)-mediated cholesterol efflux.Cells overexpressing hABCG1 were created in Chinese Hamster Ovary (CHO-K1) cells and characterized terms of lipid composition. expressed these formed homodimers was mostly present intracellularly. Cholesterol efflux from to HDL2 HDL3 increased but not lipid-free apolipoproteins. A range phospholipid containing acceptors apart high-density lipoprotein (HDL) subclasses also efficient mediating...
Rationale: High-density lipoprotein (HDL) is a heterogeneous population of particles. Differences in the capacities HDL subfractions to remove cellular cholesterol may explain variable correlations between HDL-cholesterol and cardiovascular risk inform future targets for HDL-related therapies. The ATP binding cassette transporter A1 (ABCA1) facilitates efflux lipid-free apolipoprotein A-I, but majority A-I circulation transported lipidated state ABCA1-dependent individual has not been...
Abstract Dendritic cells (DCs) migrating via lymph are the primary influence regulating naive T cell differentiation, be it active immunity or tolerance. How DCs achieve this regulation in vivo is poorly understood. Intestinal direct contact with harmless pathogenic luminal contents, but may also influenced by signals from epithelial cells, macrophages, other resident immigrant cells. To understand role of TLR7 and TLR8 intestinal DC function, we fed a TLR7/8 ligand (resiquimod (R-848)) to...
TNF-related apoptosis-inducing ligand (TRAIL) is implicated in the regulation of diabetes and reduced patients with cardiovascular disease. Although TRAIL receptors are widespread, can promote cell proliferation apoptosis, it not known how might protect against atherosclerosis. We examined development atherosclerosis Apoe −/−, Trail (also as Tnfsf10) −/− mice that were fed a high-fat diet. Plasma cholesterol, triacylglycerol, glucose insulin, well changes various metabolic enzymes regulators...
Monitoring enzyme secretion in tissue culture has proved challenging because to date the activity cannot be continuously measured situ. In this Letter, we present a solution using biopolymer loaded photonic crystals of anodized silicon. Shifts optical response by proteolytic degradation provide label-free sensing with unprecedented low detection limits (1 pg) and calculation kinetic parameters. The enhancement sensitivity relative previous crystal sensors constitutes change paradigm here...
The osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) cytokine system, not only controls bone homeostasis, but has been implicated in regulating vascular calcification. TNF–related apoptosis-inducing (TRAIL) is a second for OPG, although its effect calcification vitro controversial, role vivo yet established. This study aimed to investigate the TRAIL using smooth muscle cells (VSMCs) isolated from TRAIL−/− wild-type mice, as well vivo, advanced atherosclerotic...
Background Tumor necrosis factor–related apoptosis‐inducing ligand ( TRAIL ) has the ability to inhibit angiogenesis by inducing endothelial cell death, as well being able promote pro‐angiogenic activity in vitro. These seemingly opposite effects make its role ischemic disease unclear. Using Trail −/− and wildtype mice, we sought determine of neovascularization following hindlimb ischemia. Methods Results Reduced vascularization assessed real‐time 3‐dimensional Vevo ultrasound imaging CD 31...
Tumor necrosis-factor-related apoptosis-inducing ligand (TRAIL) has been implicated in angiogenesis; the growth of new blood vessels from an existing vessel bed. Our aim was to compare pro-angiogenic responses TRAIL, vascular endothelial growth-factor-A (VEGF-A) and fibroblast growth-factor-2 (FGF-2) either separately (10 ng/mL) or combination, followed by assessment proliferation, migration tubule formation using human microvascular endothelial-1 (HMEC-1) cells vitro. Angiogenesis also...
Abstract Background Endothelial dysfunction is a hallmark feature of cardiovascular disease (CVD), yet the underlying mechanisms are still poorly understood. This has impeded development effective therapies, particularly for peripheral artery disease. FK506-binding protein like (FKBPL) and its therapeutic peptide mimetic, AD-01, crucial negative regulators angiogenesis, however their roles in CVD unknown. In this study, we aimed to elucidate FKBPL-mediated involved regulating endothelial...
Non-alcoholic fatty liver disease (NAFLD) incorporates steatosis, non-alcoholic steato-hepatitis (NASH) and cirrhosis, associating with diabetes cardiovascular (CVD). TNF-related apoptosis-inducing ligand (TRAIL) is protective of CVD. We aimed to determine whether TRAIL protects against insulin resistance, NAFLD vascular injury. Twelve-week high fat diet (HFD)-fed Trail -/- mice had increased plasma cholesterol, glucose compared wildtype. Insulin tolerance was impaired TRAIL-deletion,...
Atherosclerosis is a chronic inflammatory disease of the vasculature characterised by infiltration activated neutrophils and macrophages at sites damage within vessel wall, which contributes to lesion formation plaque progression. Selenomethionine (SeMet) an organic form selenium (Se), essential trace element that functions in regulation immune response both bolstering endogenous thioredoxin glutathione antioxidant defence systems directly scavenging damaging oxidant species. This study...
Background and Aims. Apolipoprotein A-I (ApoA-I), the main component of high-density lipoprotein (HDL), not only promotes reverse cholesterol transport (RCT) in atherosclerosis but also increases insulin secretion pancreatic β-cells, suggesting that interventions which raise HDL levels may be beneficial diabetes-associated cardiovascular disease (CVD). Previously, we showed TNF-related apoptosis-inducing ligand (TRAIL) deletion Eknockout (Apoe-/-) mice results diabetes-accelerated response...
Journal Article Hypoxia regulates the production and activity of glucose transporter‐1 indoleamine 2,3‐dioxygenase in monocyte‐derived endothelial‐like cells: possible relevance to infantile haemangioma pathogenesis Get access A. Herbert, Herbert Lowy Cancer Research Centre Prince Wales Clinical School, University New South Wales, Sydney, NSW 2052, Australia Search for other works by this author on: Oxford Academic Google Scholar H. Ng, Ng AustraliaSydney Children's Hospital, Randwick, 2031,...
Reversal of ischemia is mediated by neo-angiogenesis requiring endothelial cell (EC) and pericyte interactions to form stable microvascular networks. We describe an unrecognized role for tumor necrosis factor–related apoptosis-inducing ligand (TRAIL) in potentiating vessel stabilization. show that the endothelium a major source TRAIL healthy circulation compromised peripheral artery disease (PAD). EC deletion vivo or vitro inhibited neo-angiogenesis, recruitment, stabilization, resulting...
Peripheral artery disease (PAD) is caused by blocked arteries due to atherosclerosis and/or thrombosis which reduce blood flow the lower limbs. It results in major morbidity, including ischemic limb, claudication, and amputation, with patients also suffering a heightened risk of heart attack, stroke, death. Recent studies suggest women have higher prevalence PAD than men, worse outcomes after intervention. In addition potential unconscious bias faced health system, underdiagnosis, rates...
<title>Abstract</title> <bold>Aims:</bold> FK506-binding protein like (FKBPL) and its therapeutic peptide mimetic, AD-01, are crucial negative regulators of angiogenesis, yet the underlying mechanisms involved in hypoxia- and/or inflammation-induced cardiovascular disease (CVD) remain unclear. We investigated whether FKBPL regulates endothelial dysfunction induced by hypoxia or inflammation if AD-01 can maintain cell function vascular integrity under these conditions. <bold>Methods Results:...
Background We recently demonstrated that TNF-related apoptosis-inducing ligand (TRAIL) is protective of diet-induced diabetes in mice. While TRAIL has been implicated chronic kidney disease, its role vivo diabetic nephropathy not clear. The present study investigated the pathogenesis using TRAIL-/-ApoE-/- Methods and ApoE-/- mice were fed a high fat diet for 20 w. Plasma glucose insulin levels assessed over 0, 5, 8 At w, markers function including creatinine, phosphate, calcium cystatin C...