A5011656613- Cholesterol and Lipid Metabolism
- Peroxisome Proliferator-Activated Receptors
- Lipoproteins and Cardiovascular Health
- Adipokines, Inflammation, and Metabolic Diseases
- Drug Transport and Resistance Mechanisms
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cancer, Lipids, and Metabolism
- Atherosclerosis and Cardiovascular Diseases
- Protease and Inhibitor Mechanisms
- Blood properties and coagulation
- Endoplasmic Reticulum Stress and Disease
- Adipose Tissue and Metabolism
- Cellular transport and secretion
- Lipid metabolism and biosynthesis
- Signaling Pathways in Disease
- Pancreatic function and diabetes
- Sphingolipid Metabolism and Signaling
- Blood Coagulation and Thrombosis Mechanisms
- Alzheimer's disease research and treatments
- Inflammasome and immune disorders
- Renal Transplantation Outcomes and Treatments
- Lipid metabolism and disorders
- Immune cells in cancer
- Bone Metabolism and Diseases
- Diabetes and associated disorders
Anzac Research Institute
2014-2024
The University of Sydney
2014-2024
Concord Repatriation General Hospital
2012-2024
Sydney Local Health District
2024
UNSW Sydney
2006-2017
Baker Heart and Diabetes Institute
2016
Leiden University Medical Center
2016
Hudson Institute of Medical Research
2016
Inserm
1999-2015
Sorbonne Université
2007-2015
To study the acceptor specificity for human ABCG1 (hABCG1)-mediated cholesterol efflux.Cells overexpressing hABCG1 were created in Chinese Hamster Ovary (CHO-K1) cells and characterized terms of lipid composition. expressed these formed homodimers was mostly present intracellularly. Cholesterol efflux from to HDL2 HDL3 increased but not lipid-free apolipoproteins. A range phospholipid containing acceptors apart high-density lipoprotein (HDL) subclasses also efficient mediating...
Rationale: High-density lipoprotein (HDL) is a heterogeneous population of particles. Differences in the capacities HDL subfractions to remove cellular cholesterol may explain variable correlations between HDL-cholesterol and cardiovascular risk inform future targets for HDL-related therapies. The ATP binding cassette transporter A1 (ABCA1) facilitates efflux lipid-free apolipoprotein A-I, but majority A-I circulation transported lipidated state ABCA1-dependent individual has not been...
Androgen abuse impairs male reproductive and cardiac function, but the rate, extent, determinants of recovery are not understood.To investigate function after ceasing androgen intake in current past abusers compared with healthy non-users.Cross-sectional, observational study recruited via social media 41 31 users (≥3 months since last use, median 300 days use) 21 healthy, eugonadal non-users. Each provided a history, examination, serum semen sample underwent testicular ultrasound, body...
Fibrinogen is a coagulation factor and an acute phase reactant up-regulated by inflammatory cytokines, such as interleukin 6 (IL-6). Elevated plasma fibrinogen levels are associated with coronary heart diseases. Fibrates clinically used hypolipidemic drugs that act via the nuclear receptor peroxisome proliferator-activated α (PPARα). In addition, most fibrates also reduce levels, but molecular mechanism unknown. this study, we demonstrate decrease basal IL-6-stimulated expression of human...
Macrophages, cytokines, and matrix metalloproteinases (MMP) play important roles in atherogenesis. The Ca(2+)-binding protein S100A12 regulates monocyte migration may contribute to atherosclerosis by inducing proinflammatory cytokines macrophages. We found significantly higher levels sera from patients with coronary artery disease than controls correlated positively C-reactive protein. was released into the circulation ruptured plaque acute syndrome, after mechanical disruption percutaneous...
Apolipoprotein E (apoE) is a 34-kDa glycoprotein secreted from various cells including hepatocytes and macrophages plays an important role in remnant lipoprotein clearance, immune responses, Alzheimer disease, atherosclerosis. Cellular apoE plasma exist as multiple glycosylated sialylated glycoforms with being less glycosylated/sialylated than cell-derived apoE. Some of the glycan structures on are characterized; however, more complicated cellular/secreted remain unidentified. We...
Journal Article Hypoxia regulates the production and activity of glucose transporter‐1 indoleamine 2,3‐dioxygenase in monocyte‐derived endothelial‐like cells: possible relevance to infantile haemangioma pathogenesis Get access A. Herbert, Herbert Lowy Cancer Research Centre Prince Wales Clinical School, University New South Wales, Sydney, NSW 2052, Australia Search for other works by this author on: Oxford Academic Google Scholar H. Ng, Ng AustraliaSydney Children's Hospital, Randwick, 2031,...
Cholesterol-loaded foam cell macrophages are prominent in atherosclerotic lesions and play complex roles both inflammatory signaling lipid metabolism, which underpinned by large scale reprogramming of gene expression. We performed a microarray study primary human that showed transcription the sphingomyelin phosphodiesterase acid-like 3A (SMPDL3A) is up-regulated after cholesterol loading. SMPDL3A protein expression secretion from stimulated loading, liver X receptor ligands, cyclic AMP,...
Macrophage-specific expression of apolipoprotein (apo)E protects against atherosclerosis; however, the signaling and trafficking pathways regulating secretion apoE are unknown. We investigated roles actin skeleton, microtubules, protein kinase A (PKA) calcium (Ca2+) in from macrophages. Disrupting microtubules with vinblastine or colchicine inhibited basal substantially, whereas disruption skeleton had no effect. Structurally distinct inhibitors PKA (H89, KT5720, inhibitory peptide...
This study was aimed at evaluating the relationship between visceral fat accumulation and plasma plasminogen activator inhibitor-1 (PAI-1) levels in healthy, obese men women undergoing weight loss therapy. The subjects, 25 premenopausal women, aged 26 49 years, with an initial body mass index 28 38 kg/m2, received a controlled diet for 13 weeks providing 4.2 MJ/day energy deficit. Magnetic resonance imaging used to measure subcutaneous abdominal fat. Our results show that before...
Cyclosporin A (CsA) is an immunosuppressant commonly used to prevent organ rejection but associated with hyperlipidemia and increased risk of cardiovascular disease. Although studies suggest that CsA-induced mediated by inhibition low-density lipoprotein receptor (LDLr)-mediated clearance, the data supporting this are inconclusive. We therefore sought investigate role LDLr in using Ldlr-knockout mice (Ldlr(-/-)).Ldlr(-/-) wild-type (wt) C57Bl/6 were treated 20 mg/kg per d CsA for 4 weeks. On...
Apolipoprotein A-I (apoA-I)-mediated cholesterol efflux involves the binding of apoA-I to plasma membrane via its C terminus and requires cellular ATP-binding cassette transporter (ABCA1) activity. ApoA-I also stimulates secretion apolipoprotein E (apoE) from macrophage foam cells, although mechanism this process is not understood. In study, we demonstrate that apoE independently both ABCA1-mediated lipid by terminus. Pulse-chase experiments using 35S-labeled exists in relatively mobile (Em)...
Cholesterol excess is typical of various diseases including atherosclerosis. We have investigated whether cholesterol accumulation in the ER (endoplasmic reticulum) can inhibit exit vesicular cargo and secretion proteins by studying apoE (apolipoprotein E), a significant glycoprotein human health disease. CHO (Chinese hamster ovary) cells expressing under cholesterol-independent promoter incubated with cholesterol–cyclodextrin complexes showed increased levels cellular free esterified...
The immediate-early gene Egr-1 controls the inducible expression of many genes implicated in pathogenesis a range vascular disorders, yet our understanding mechanisms controlling rapid this prototypic zinc finger transcription factor is poor. Here we show that induced by IL-1beta dependent on metalloproteinases (MMP) and disintegrin metalloproteinase (ADAM). Pharmacologic MMP/ADAM inhibitors siRNA knockdown prevent induction Egr-1. Further, activates via epidermal growth receptor (EGFR)....