A5017413531- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- S100 Proteins and Annexins
- Advanced Fluorescence Microscopy Techniques
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Lipid Membrane Structure and Behavior
- Immunotherapy and Immune Responses
- Advanced Electron Microscopy Techniques and Applications
- Glycosylation and Glycoproteins Research
- Advanced biosensing and bioanalysis techniques
- Protein Tyrosine Phosphatases
- Immune Response and Inflammation
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Advanced Biosensing Techniques and Applications
- Galectins and Cancer Biology
- Blood Coagulation and Thrombosis Mechanisms
- Asthma and respiratory diseases
- Cellular transport and secretion
- Diffusion and Search Dynamics
- Graphite, nuclear technology, radiation studies
- Digital Holography and Microscopy
- Complement system in diseases
- Chemical Synthesis and Analysis
UNSW Sydney
2016-2025
University of Oxford
2012-2024
EMBL Australia
2016-2024
ARC Centre of Excellence in Advanced Molecular Imaging
2016-2022
Australian Research Council
2022
University of Aberdeen
2014
Abstract Live-cell super-resolution microscopy enables the imaging of biological structure dynamics below diffraction limit. Here we present enhanced radial fluctuations (eSRRF), substantially improving image fidelity and resolution compared to original SRRF method. eSRRF incorporates automated parameter optimization based on data itself, giving insight into trade-off between fidelity. We demonstrate across a range modalities systems. Notably, extend three dimensions by combining it with...
CD4+ T cells are vital for host defense and immune regulation. However, the fundamental role of CD4 itself remains enigmatic. We report seven patients aged 5–61 years from five families four ancestries with autosomal recessive deficiency a range infections, including recalcitrant warts Whipple’s disease. All homozygous rare deleterious variants impacting expression canonical isoform. A shorter expressed isoform that interacts LCK, but not HLA class II, is affected by only one variant. lack...
Abstract C-reactive protein (CRP) and serum amyloid A (SAA) increase in the blood of patients with inflammatory conditions CRP-induced monocyte tissue factor (TF) may contribute to inflammation-associated thrombosis. This study demonstrates that SAA is a potent rapid inducer human TF. induced TF mRNA PBMC within 30 min optimal procoagulant activity 4 h, whereas CRP (25 μg/ml)-induced was minimal at this time. Unlike CRP, did not synergize LPS. Procoagulant inhibited by anti-TF dependent on...
S100A12 is expressed at sites of acute, chronic, and allergic inflammation. S100 proteins have regions high sequence homology, but the "hinge" region between conserved calcium binding domains structurally functionally divergent. Because murine S100A8 hinge domain (mS100A842–55) a monocyte chemoattractant whereas human (hS100A843–56) inactive, we postulated that common hydrophobic amino acids within may be functional. The domain, S100A1238–53, was chemotactic for monocytes mast cells in...
Macrophages, cytokines, and matrix metalloproteinases (MMP) play important roles in atherogenesis. The Ca(2+)-binding protein S100A12 regulates monocyte migration may contribute to atherosclerosis by inducing proinflammatory cytokines macrophages. We found significantly higher levels sera from patients with coronary artery disease than controls correlated positively C-reactive protein. was released into the circulation ruptured plaque acute syndrome, after mechanical disruption percutaneous...
Reactive oxygen species generated by activated neutrophils can cause oxidative stress and tissue damage. S100A8 (A8) S100A9 (A9), abundant in neutrophil cytoplasm, are exquisitely sensitive to oxidation, which may alter their functions. Murine A8 is a chemoattractant, but it suppresses leukocyte transmigration the microcirculation when S-nitrosylated. Glutathione (GSH) modulates intracellular redox, S-glutathionylation protect susceptible proteins from damage regulate function. We...
Superresolution techniques have advanced our understanding of complex cellular structures and processes but require the attachment fluorophores to targets through tags or antibodies, which can be bulky result in underlabeling. To overcome these limitations, we developed a technique visualize nanoscale binding locations signaling proteins by taking advantage their native interaction domains. Here, demonstrated that pPAINT (protein point accumulation topography) is new, single-molecule...
Significance Src homology 2 (SH2) domains are phosphotyrosine binding motifs that play key roles in cellular signaling. There 110 proteins the human genome containing SH2 domains, of which 10 contain tandem domains. Tandem have been shown to improve avidity and specificity contribute allostery. Here, we show can also exhibit lifetimes accelerated by activity phosphatases. This unbinding requires engage their substrates dynamic modes cycle between single SH2-bound states. We experimentally...
In recent years, the development of new image analysis approaches has highlighted possibility recovering superresolution information from short sequences wide-field images. Our recently developed method, SRRF (Super-Resolution Radial Fluctuations), enables long-term live-cell imaging beyond resolution limit without specialized hardware. Here, we present eSRRF (enhanced-SRRF), a significant improvement over our initial enhancing fidelity to underlying structure and resolution. Especially,...