A5064331067- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Shoulder Injury and Treatment
- Hearing, Cochlea, Tinnitus, Genetics
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Orthopedic Surgery and Rehabilitation
- Foot and Ankle Surgery
- Plasma Applications and Diagnostics
- Orthopaedic implants and arthroplasty
- Musculoskeletal pain and rehabilitation
- Total Knee Arthroplasty Outcomes
- Advanced biosensing and bioanalysis techniques
- Signaling Pathways in Disease
- Spine and Intervertebral Disc Pathology
- Plasma Diagnostics and Applications
- Cardiomyopathy and Myosin Studies
- Streptococcal Infections and Treatments
- Tendon Structure and Treatment
- Hip and Femur Fractures
- Advanced Fluorescence Microscopy Techniques
- Cell Adhesion Molecules Research
- Orthopedic Infections and Treatments
- Optical Coherence Tomography Applications
- Spinal Fractures and Fixation Techniques
UNSW Sydney
2015-2025
Beaumont Hospital
2014-2024
EMBL Australia
2017-2024
ARC Centre of Excellence in Advanced Molecular Imaging
2018-2022
University of Technology Sydney
2014-2019
Cappagh National Orthopaedic Hospital
2006-2017
St. Vincent's University Hospital
2009-2011
Nenagh Hospital
2009
Our Lady's Hospital
2006-2009
22q11 Ireland
2007
The HIV-1 capsid has emerged as a tractable target for antiretroviral therapy. Lenacapavir, developed by Gilead Sciences, is the first capsid-targeting drug approved medical use. Here, we investigate effect of lenacapavir on HIV stability and uncoating. We employ single particle approach that simultaneously measures content release lattice persistence. demonstrate lenacapavir's potent antiviral activity predominantly due to lethal hyperstabilisation resultant loss compartmentalisation. This...
ABSTRACT The mouse shaker-1 locus, Myo7a, encodes myosin VIIA and mutations in the orthologous gene humans cause Usher syndrome type 1B or non-syndromic deafness. Myo7a is expressed very early sensory hair cell development inner ear. We describe effects of three on cochlear function. In Myo7a816SB Myo7a6J mutants, stereocilia grow form rows graded heights as normal, but bundles become progressively more disorganised. Most these mutants show no gross electrophysiological responses, some did...
Background: Minimally invasive surgery is being used increasingly, including for hallux valgus surgery. Despite the growing interest in minimally procedures, there have been few publications on percutaneous chevron/akin (PECA) and no studies published comparing PECA to open scarf/akin osteotomies (SA). Methods: This was a prospective, randomized study of 50 patients undergoing operative correction using one 2 techniques (PECA vs SA). Data were collected preoperatively 1 day, weeks, 6 months...
Uncoating of the metastable HIV-1 capsid is a tightly regulated disassembly process required for release viral cDNA prior to nuclear import. To understand intrinsic pathway and how it can be modulated, we have developed single-particle fluorescence microscopy method follow real-time uncoating kinetics authentic HIV capsids in vitro immediately after permeabilizing membrane. Opening first defect lattice rate-limiting step uncoating, which followed by rapid, catastrophic collapse. The...
Numerous mammalian diseases have been found to be due mutations in components of the actin cytoskeleton. Recently, gene for an unconventional myosin, myosin-VIIa, were basis deafness and vestibular dysfunction observed shaker-1 (sh1) mice a human deafness–blindness syndrome, Usher syndrome type 1B. Seven alleles sh1 analyzed assess affects different myosin-VIIa on both expression tissue function. Myosin-VIIa is expressed inner ear retina, as well kidney, lung, testis. Northern blot analysis...
The shaker‐1 ( Myo7a ) mouse deafness locus is encoded by an unconventional myosin gene: VIIA [Gibson, Walsh, Mburu, Varela, Brown, Antonio, Biesel, Steel and Brown (1995) Nature (London) 374 , 62–64]. gene expressed in hair cells the cochlea, where it thought to function development of critical neuroepithelium auditory transduction takes place. In order understand better VIIA, we have determined complete sequence cDNA employed wild‐type for mutational analysis a number alleles. Analysis...
One mechanism for achieving accurate placement of the cell division machinery is via Turing patterns, where nonlinear molecular interactions spontaneously produce spatiotemporal concentration gradients. The resulting patterns are dictated by shape. For example, Min system Escherichia coli shows oscillation between poles, leaving a mid-cell zone division. universality pattern-forming mechanisms in divisome currently unclear. We examined location plane two pleomorphic archaea, Haloferax...
Abstract Two-photon direct laser writing is an additive fabrication process that utilizes two-photon absorption of tightly focused femtosecond pulses to implement spatially controlled polymerization a liquid-phase photoresist. capable nanofabricating arbitrary three-dimensional structures with nanometer accuracy. Here, we explore for high-resolution optical microscopy by fabricating unique 3D fiducials single-molecule tracking and localization microscopy. By having control over the position...
Ezrin is a member of the ERM (ezrin–radixin–moesin) family proteins that have been conserved through metazoan evolution. These dormant and active forms, where latter links actin cytoskeleton to membranes. three domains: an N-terminal FERM [band Four-point-one (4.1) ERM] domain comprising subdomains (F1, F2, F3); helical domain; C-terminal actin-binding domain. In form, domains form stable complex. We determined crystal structures FERM:C-terminal complex human ezrin. observe bistable array...
Modern fluorescence microscopy requires software-controlled illumination sources with high power across a wide range of wavelengths. Diode lasers meet the requirements and combining multiple units into single fiber launch expands their capability required spectral range. We present NicoLase, an open-source diode laser combiner, launch, software sequence controller for super-resolution applications. Two configurations are described, giving four or six output wavelengths one two single-mode...
MinD proteins are well studied in rod-shaped bacteria such as E. coli, where they display self-organized pole-to-pole oscillations that important for correct positioning of the Z-ring at mid-cell cell division. Archaea also encode belonging to family, but their functions unknown. homologous were found be widespread Euryarchaeota and form a sister group bacterial distinct from ParA other related ATPase families. We aimed identify function four archaeal model archaeon Haloferax volcanii....
Heavy weight lifting using a squat bar is commonly used athletic training exercise. Previous in vivo motion studies have concentrated on of everyday objects and not the vastly increased loads that athletes subject themselves to when performing this exercise.Athletes significantly alter their lumbar spinal at heavy weights.Controlled laboratory study.Forty-eight (28 men, 20 women) performed 6 lifts 40% maximum, 4 60% 2 80% maximum. The Zebris 3D analysis system was measure spine motion....
Abstract No systems have been reported for genetic manipulation of cold-adapted Archaea . Halorubrum lacusprofundi is an important member Deep Lake, Antarctica (~10% the population), and amendable to laboratory cultivation. Here we report development a shuttle-vector targeted gene-knockout system this species. To investigate function acetamidase/formamidase genes, class genes not experimentally studied in , acetamidase gene, amd3 was disrupted. The wild-type grew on acetamide as sole source...
Efficient HIV-1 replication depends on interaction of the viral capsid with host protein cyclophilin A (CypA). CypA, a peptidylprolyl isomerase, binds to an exposed loop in CA via enzyme's active site. Recent structural analysis CypA complex tubes conjunction molecular dynamics simulations identified secondary binding site that allows bridging two hexameric subunits assembled lattice, leading stabilization (Liu et al. Nat Commun 7:10714, 2016). We performed mutational residues have been...