A5033226873- Birth, Development, and Health
- Animal Nutrition and Physiology
- Gestational Diabetes Research and Management
- Epigenetics and DNA Methylation
- Folate and B Vitamins Research
- Diet and metabolism studies
- Animal Behavior and Welfare Studies
- Adipose Tissue and Metabolism
- Meat and Animal Product Quality
- Pregnancy and preeclampsia studies
- Fatty Acid Research and Health
- Health, Environment, Cognitive Aging
- Obesity, Physical Activity, Diet
- Ovarian function and disorders
- Liver Disease Diagnosis and Treatment
- Diet, Metabolism, and Disease
- Metabolism and Genetic Disorders
- Ruminant Nutrition and Digestive Physiology
- Physical Activity and Health
- Pancreatic function and diabetes
- Eating Disorders and Behaviors
- Regulation of Appetite and Obesity
- RNA Research and Splicing
- Child Nutrition and Water Access
- Psychotherapy Techniques and Applications
UNSW Sydney
2012-2022
Victor Chang Cardiac Research Institute
2010-2013
St Vincent's Hospital Sydney
2013
Laboratory of Molecular Genetics
2013
University of Aberdeen
2010
University of Utah
2010
The University of Sydney
2003-2009
Kansas State University
2003-2004
University of Illinois Urbana-Champaign
2000
The Ohio State University
2000
A complex combination of adult health-related disorders can originate from developmental events that occur in utero . The periconceptional period may also be programmable. We report on the effects restricting supply specific B vitamins (i.e., 12 and folate) methionine, within normal physiological ranges, diet mature female sheep. hypothesized this would lead to epigenetic modifications DNA methylation preovulatory oocyte and/or preimplantation embryo, with long-term health implications for...
The gut microbiota is emerging as a new factor in the development of obesity. Many studies have described changes composition response to obesity and high fat diet (HFD) at phylum level. In this study we used 16s RNA throughput sequencing on faecal samples from rats chronically fed HFD or control chow (n = 10 per group, 16 weeks) investigate species 53.17% dissimilarity between groups was observed Lactobacillus intestinalis dominated under diet. However considerably less abundant (P<0.0001),...
We previously showed that paternal high-fat diet (HFD) consumption programs β-cell dysfunction in female rat offspring, together with transcriptome alterations islets. Here we investigated the retroperitoneal white adipose tissue (RpWAT) using gene and pathway enrichment analysis to determine whether commonly affected network topologies exist between these two metabolically related tissues. In RpWAT, 5108 genes were differentially expressed due a HFD; top 5 significantly enriched networks...
Intrauterine nutrition can program metabolism, creating stable changes in physiology that may have significant health consequences. The mechanism underlying these is widely assumed to involve epigenetic the expression of metabolic genes, but evidence supporting this idea limited. Here we performed first study epigenomic consequences exposure maternal obesity and diabetes. We used a mouse model natural-onset allows comparison genetically identical mice whose mothers were either obese diabetic...
The Sp/Krüppel-like factor (Sp/Klf) family is comprised of around 25 zinc finger transcription factors that recognize CACCC boxes and GC-rich elements. We have investigated basic Krüppel-like (Bklf/Klf3) show in erythroid tissues its expression highly dependent on another member, (Eklf/Klf1). observe Bklf mRNA significantly reduced from Eklf-null murine embryos. find driven primarily by two promoters, a ubiquitously active upstream promoter, 1a, an downstream 1b. Transcripts the promoters...
Beyond the short-term effects on fertility, there is increasing evidence that obesity or consumption of an inappropriate diet by mother during pregnancy adversely affects long-term health her offspring. PPAR and RXR isotypes are widely expressed in reproductive tissues developing fetus. Through their interactions with fatty acids, they may mediate adaptive responses to changes maternal diet. In maturing follicle, PPAR-gamma has important role granulosa cells surround oocyte. After...
Along with diabetes and obesity, chronic kidney disease (CKD) is increasing across the globe. Although some data support an effect of maternal obesity on offspring kidney, impact paternal unknown; thus, we have studied prior to conception. Male Sprague Dawley rats were fed chow diet or high fat (HFD) for 13–14 weeks before mating chow-fed females. weaned onto killed at 27 renal gene expression histology. Fathers HFD 30% heavier than Controls mating. At age obese fathers weighed 10% less;...
Emerging evidence suggests that paternal obesity plays an important role in offspring health. Our previous work using a rodent model of diet-induced showed female from high-fat diet (HFD)-fed fathers develop glucose intolerance due to impairment pancreatic insulin secretion. Here, we focused on the health outcomes male HFD-fed fathers. Male Sprague-Dawley rats (3 wk old) were fed control (CD-F0) or HFD (HFD-F0) for 12 before mating with control-fed females. diets up 8 6 mo. Although HFD-F0...
Emerging evidence from rodent studies suggests that high-fat-diet (HFD)-induced obesity is characterized by increased oxidative damage in sperm and testis. However, interventions using micronutrient supplementation to mitigate have not been extensively studied. This study aimed investigate the effect of an antioxidant-based supplement (added folate, vitamin B6, choline, betaine, zinc) on testicular HFD-fed male Sprague Dawley rats.Rats (3-weeks-old, 12/group) were weaned onto control (C) or...
Two experiments were conducted to determine the effects of rancidity and FFA in choice white grease (CWG) on growth performance nutrient digestibility nursery pigs. In Exp. 1,150 crossbred pigs (average initial BW 6.8 kg average age 21 d) used. Treatments (as-fed basis) a corn-soybean meal-based control with no added fat, 6% CWG, CWG heated at 80 degrees C, oxygen gas bubbled through it 849 mL/min for 5, 7, 9, or 11 d. Peroxide value increased as oxidative exposure was from 0 7 d (i.e.,...
The methionine cycle and methyl group metabolism are implicated in the long-term programming of metabolism. Diets deficient folic acid, choline have been fed to pregnant rats examine effects on amino acid metabolism, reserves DNA methylation dam fetuses. Animals were folate-deficient, folate-deficient with low methionine, low-methionine, low-choline diets starting 2 weeks before mating. dams their fetuses subsequently killed day 21 gestation for analysis. reduced fetal maternal weight....
The pathogenic agent responsible for the expanded repeat diseases, a group of neurodegenerative diseases that includes Huntington's disease is not yet fully understood. Expanded polyglutamine (polyQ) thought to be toxic in certain cases, however, all genes can encode polyQ sequence. Since repeat-containing RNA intermediary common these hairpin-forming single-stranded has been investigated as potential agent. More recently, it become apparent most loci have transcription occurring from both...
Abstract Objective : Intrauterine growth restriction is associated with increased prevalence of the metabolic syndrome in adult life, including adiposity. The aim this study was to investigate if maternal protein energy malnutrition changes expression genes involved fat partitioning weanling rats. Research Methods and Procedures Time‐mated mothers were placed on one two isocaloric diets, low [(LP), 8% protein] or control (20% protein). All remained diet throughout pregnancy lactation. A...
There is now strong evidence that the paternal contribution to offspring phenotype at fertilisation more than just DNA. However, identity and mechanisms of this nongenetic inheritance are poorly understood. One important questions in research area is: do changes sperm DNA methylation have phenotypic consequences for offspring? We previously reported obese male rats altered glucose metabolism compared with controls effect was inherited through means. Here, we describe investigations into a...
Unhealthy diets, and ensuing weight gain, predispose individuals to the development of esophageal adenocarcinoma. We examined effect chronic high fat diet (HFD) on microbiota Sprague Dawley rats using Illumina MiSeq amplicon sequencing (V4, 515 F/806 R) expression IL18, PTGS2, PPARA, FFAR3, CRAT. The relationships among metabolic parameters, microbiota, host gene were determined. observed a significant difference between upper lower in control fed rats, emphasized by enrichment Lactobacillus...
The importance of folic acid and the methionine cycle in fetal development is well recognised even though mechanism has not been established. Since active maternal liver, poor folate status may modify hepatic metabolism. Pregnant rats were fed diets deficient (-F) or three key methyl donors, acid, choline (-FLMLC) liver was analysed on day 21 gestation. Two-dimensional gel electrophoresis soluble proteins identified differentially abundant proteins, which could be allocated into nine...
Abstract Background Respiratory Syncytial Virus (RSV), a major cause of bronchiolitis, has large impact on the census pediatric hospitals during outbreak seasons. Reliable prediction week these outbreaks will start, based readily available data, could help better prepare for outbreaks. Methods Naïve Bayes (NB) classifier models were constructed using weather data from 1985-2008 considering only variables that are in real time and be used to forecast which an RSV occur Salt Lake County, Utah....