A5040306520- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Glycosylation and Glycoproteins Research
- Proteoglycans and glycosaminoglycans research
- Collagen: Extraction and Characterization
- Advanced biosensing and bioanalysis techniques
- Bone Tissue Engineering Materials
- Connective tissue disorders research
- Algal biology and biofuel production
- Conducting polymers and applications
- Nanopore and Nanochannel Transport Studies
- Electrospun Nanofibers in Biomedical Applications
- Chemistry and Chemical Engineering
- Hydrogels: synthesis, properties, applications
- Polyoxometalates: Synthesis and Applications
- Supramolecular Self-Assembly in Materials
- Advanced Sensor and Energy Harvesting Materials
- Bacteriophages and microbial interactions
- Cancer Cells and Metastasis
- Microfluidic and Bio-sensing Technologies
- Pancreatic function and diabetes
- Innovative Microfluidic and Catalytic Techniques Innovation
- Adenosine and Purinergic Signaling
- Silk-based biomaterials and applications
- Neurobiology and Insect Physiology Research
University of Milano-Bicocca
2020-2025
Three-dimensional (3D) bioprinting allows the production of artificial 3D cellular microenvironments thanks to controlled spatial deposition bioinks. Proper bioink characterization is required achieve essential characteristics printability and biocompatibility for bioprinting. In this work, a protocol standardize experimental new proposed. A functionalized hydrogel based on gelatin chitosan was used. The divided into three steps: pre-printing, bioprinting, post-printing. For pre-printing...
The Diels–Alder (DA) cycloaddition is one of the best methods for biomaterials conjugation to generate tools biomedical applications. It a biocompatible one‐step reaction, exploiting functional groups absent in natural biopolymers, doesn't require catalysts, and side products, an ideal “click reaction”. In this review we provide overview on recent examples which DA has been exploited nanomedicine. Nanoparticles drug delivery or diagnosis, nanostructured regenerative medicine, have...
Alginate-gelatin hydrogels mimicking extracellular matrix (ECM) of soft tissues have been generated by static-dynamic double crosslinking, allowing fine control over the physical and chemical properties. Dynamic crosslinking provides self-healing injectability attributes to hydrogel promotes cell migration proliferation, while static network improves stability. The was performed enzymatic coupling tyrosine residues gelatin with tyramine inserted in alginate backbone, catalyzed horseradish...
In cancer microenvironment, aberrant glycosylation events of ECM proteins and cell surface receptors occur. We developed a protocol to generate 3D bioprinted models colorectal (CRC) crosslinking hyaluronic acid gelatin functionalized with three signalling glycans characterized in CRC, 3'-Sialylgalactose, 6'-Sialylgalactose 2'-Fucosylgalactose. The crosslinking, performed exploiting azide 4arm-PEG-dibenzocyclooctyne, resulted biocompatible hydrogels that were commercial CRC cells HT-29...
Abstract Bioactive films composed of Spiro‐OMeTAD, a conductive molecular material (CMM), in combination with collagen have been manufactured and characterised for the first time. In‐vitro cellular testing demonstrated non‐cytotoxicity doped Spiro‐OMeTAD /Collagen films, opening way implantable or wearable medical devices biosensors based on materials.
Cell microenvironment contains a plethora of information that influences cell modulation. Indeed, the extracellular matrix plays central role in tissue development. Reproducing cell-extracellular crosstalk able to recapitulate both physical and biochemical signals is crucial obtain functional models or regenerative strategies.
Abstract The generation of 3D‐bioprintable and biocompatible hydrogels based on elastin hyaluronic acid is described. procedure a click reaction between maleimide thiol for the final crosslinking, employable with living cells due to fast kinetics absence side products. To this end, both α‐elastin were functionalized linkers ending groups, at controlled functionalization intensities, crosslinked dithiol−PEG linker. Varying equivalents three reagents, four different obtained their...
The development of human tissue models for regenerative medicine and animal-free drug screening requires glycosylated biomaterials such as collagen. An easy fast biomaterial glycosylation method exploiting Horseradish Peroxidase (HRP) phenol coupling reaction is proposed. protocol adaptable to any polymer functionalized with residues or tyrosine containing proteins. As a model the on collagen films were salidroside, natural β-glucoside in aglycone. Scanning Electron Microscope (SEM) contact...