A5066260392- Epigenetics and DNA Methylation
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Genetics and Neurodevelopmental Disorders
- Cancer-related gene regulation
- RNA modifications and cancer
- Plant nutrient uptake and metabolism
- Birth, Development, and Health
- Genetic Syndromes and Imprinting
- RNA Research and Splicing
- Plant Virus Research Studies
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Muscle Physiology and Disorders
- Genomic variations and chromosomal abnormalities
- Chromatin Remodeling and Cancer
- Legume Nitrogen Fixing Symbiosis
- Cancer Mechanisms and Therapy
- Immunodeficiency and Autoimmune Disorders
- Genomics and Rare Diseases
- Cancer Genomics and Diagnostics
- Plant Reproductive Biology
- RNA and protein synthesis mechanisms
- Prenatal Screening and Diagnostics
Leiden University Medical Center
2014-2024
QIMR Berghofer Medical Research Institute
2010-2023
Leiden University
2016-2023
Gregor Mendel Institute of Molecular Plant Biology
2005-2017
Institute for Molecular Science
2013-2016
La Trobe University
2015-2016
Austrian Academy of Sciences
2003-2012
Ollscoil na Gaillimhe – University of Galway
2011
University of Geneva
2009
Max Planck Institute for Plant Breeding Research
2009
Abstract The Arabidopsis genome encodes four Dicer-like (DCL) proteins, two of which contain putative nuclear localization signals. This suggests one or more pathways for processing double-stranded (ds) RNA in plants. To study the subcellular location nuclear-encoded dsRNA involved transcriptional silencing, we examined short interfering (si) and micro (mi) accumulation transgenic expressing cytoplasmic variants P19, a viral protein that suppresses posttranscriptional gene silencing. P19...
Chromosome termini form a specialized type of heterochromatin that is important for chromosome stability. The recent discovery telomeric RNA transcripts in yeast and vertebrates raised the question whether RNA-based mechanisms are involved formation heterochromatin. In this study, we performed detailed analysis chromatin structure transcription at Arabidopsis. Arabidopsis telomeres display features intermediate does not extensively spread to subtelomeric regions which encode...
Disruption of cell division cycle associated 7 (CDCA7) has been linked to aberrant DNA hypomethylation, but the impact methylation loss on transcription not investigated. Here, we show that CDCA7 is critical for maintaining global levels across multiple tissues in vivo. A pathogenic
Abstract Background We have used a sensitized ENU mutagenesis screen to produce mouse lines that carry mutations in genes required for epigenetic regulation. call these Modifiers of murine metastable epialleles ( Mommes ). Results report basic molecular and phenotypic characterization twenty the Momme lines, each case we also identify causative mutation. Three mutation novel modifier, Rearranged L-myc fusion Rlf ), one gene, Rap-interacting factor 1 Rif1 has not previously been reported be...
For genetically heterogeneous diseases a better understanding of how the underlying gene defects are functionally interconnected will be important for dissecting disease etiology. The Immunodeficiency, Centromeric instability, Facial anomalies (ICF) syndrome is chromatin disorder characterized by mutations in DNMT3B, ZBTB24, CDCA7 or HELLS Here, we generated Zbtb24 BTB domain deletion mouse and found that loss functional leads to early embryonic lethality. Transcriptome analysis identified...
Abstract Bulky DNA lesions in transcribed strands block RNA polymerase II (RNAPII) elongation and induce a genome-wide transcriptional arrest. The transcription-coupled repair (TCR) pathway efficiently removes transcription-blocking lesions, but how transcription is restored the genome following remains unresolved. Here, we find that TCR-specific CSB protein loads PAF1 complex (PAF1C) onto RNAPII promoter-proximal regions response to damage. Although dispensable for TCR-mediated repair,...
Monozygotic (MZ) twins and higher-order multiples arise when a zygote splits during pre-implantation stages of development. The mechanisms underpinning this event have remained mystery. Because MZ twinning rarely runs in families, the leading hypothesis is that it occurs at random. Here, we show strongly associated with stable DNA methylation signature adult somatic tissues. This spans regions near telomeres centromeres, Polycomb-repressed heterochromatin, genes involved cell-adhesion, WNT...
Abstract Background Loss of epigenetic control is a hallmark aging. Among the most prominent roles mechanisms inactivation one two copies X chromosome in females through DNA methylation. Hence, age-related disruption X-chromosome (XCI) may contribute to aging process women. Methods We analyzed 9,777 CpGs on whole blood samples from 2343 and 1688 males (Illumina 450k methylation array) replicated findings duplicate using purified monocyte data set (in total, 991/924 females/males). used...
Significance The Microrchidia (MORC) family of ATPases are important regulators gene silencing in multiple organisms but little is known about their molecular behavior. In this study, we used crystallography and native mass spectrometry to show that MORC3 forms dimers when it binds nonhydrolyzable ATP analogues. We also determined the CW zinc finger-like domain can bind euchromatic histone H3 lysine 4 (H3K4) methylation localizes H3K4me3-marked chromatin. crystal structure provides details...
Grainyhead like 2 (GRHL2) is an essential transcription factor for development and function of epithelial tissues. It has dual roles in cancer by supporting tumor growth while suppressing to mesenchymal transitions (EMT). GRHL2 cooperates with androgen estrogen receptors (ER) regulate gene expression. We explore genome wide binding sites conserved three ER⍺/GRHL2 positive luminal breast cell lines ChIP-Seq. Interaction the ER⍺/FOXA1/GATA3 complex observed, however, only a minor fraction...