A5045064378- Chromosomal and Genetic Variations
- Tissue Engineering and Regenerative Medicine
- Plant Virus Research Studies
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Genetic factors in colorectal cancer
- Genomics and Chromatin Dynamics
- Genetic Syndromes and Imprinting
- Immune Cell Function and Interaction
- Plant Disease Resistance and Genetics
- Educational Games and Gamification
- Phytoplasmas and Hemiptera pathogens
- Cancer Genomics and Diagnostics
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Genetics and Neurodevelopmental Disorders
- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- T-cell and B-cell Immunology
- Cell Image Analysis Techniques
Leiden University Medical Center
2020-2024
University of Amsterdam
2018-2024
Leiden University
2019-2023
Netherlands Institute for Neuroscience
2019
Disruption of cell division cycle associated 7 (CDCA7) has been linked to aberrant DNA hypomethylation, but the impact methylation loss on transcription not investigated. Here, we show that CDCA7 is critical for maintaining global levels across multiple tissues in vivo. A pathogenic
Grainyhead like 2 (GRHL2) is an essential transcription factor for development and function of epithelial tissues. It has dual roles in cancer by supporting tumor growth while suppressing to mesenchymal transitions (EMT). GRHL2 cooperates with androgen estrogen receptors (ER) regulate gene expression. We explore genome wide binding sites conserved three ER⍺/GRHL2 positive luminal breast cell lines ChIP-Seq. Interaction the ER⍺/FOXA1/GATA3 complex observed, however, only a minor fraction...
Cell fate decisions are orchestrated by an interplay of epigenetic factors in collaboration with transcriptional activators and repressors. The reader MORC3 is known as a silencer endogenous retroviruses mouse embryonic stem cells. Here, we show that expressed the thymus reveal loss function leads to severe arrest T cell development at DN1 stage, accompanied expansion natural killer myeloid depends on its ATPase ability bind H3K4me via CW-type zinc finger domain. We find altered chromatin...
Summary Crossover formation during meiosis in plants is required for proper chromosome segregation and essential crop breeding as it allows an (optimal) combination of traits by mixing parental alleles on each chromosome. commences with the production a large number DNA double‐strand breaks, which only few result crossovers. A small genes, drive resolution crossover intermediate structures towards non‐crossovers, have been identified Arabidopisis thaliana . In order to explore potential...
The major transcription factors that regulate how stem cells commit to become T lymphocytes are functionally defined
Microrchidia proteins (MORCs) are involved in epigenetic gene silencing a variety of eukaryotic organisms. Deletion MORCs result several developmental abnormalities and their dysregulation has been implicated disease multiple cancers. Specifically, mammalian MORC3 mutations associated with immune system defects human cancers such as bladder, uterine, stomach, lung, diffuse large B cell lymphomas. While previous studies have shown that binds to H3K4me3 vitro overlaps ChIP-seq peaks mouse...
ABSTRACT DNA methylation is a key epigenetic modification essential for normal development. How particular factors control patterns and activity of given locus incompletely understood. The zinc finger protein Zbtb24 has been implicated in transcriptional activation/repression the maintenance pathway. Here, using whole genome bisulfite sequencing mouse embryonic stem cells, we report that besides general trend towards hypomethylation, many genomic sites gain absence they include promoters...
Increasing natural resistance and resilience in plants is key for ensuring food security within a changing climate. Breeders improve these traits by crossing cultivars with their wild relatives introgressing specific alleles through meiotic recombination. However, some genomic regions are devoid of recombination especially crosses between divergent genomes, limiting the combinations desirable alleles. Here, we used pooled-pollen sequencing to build map recombinant non-recombinant tomato five...
Abstract Tomato is the most consumed vegetable in world. Increasing its natural resistance and resilience key for ensuring food security within a changing climate. Plant breeders improve those traits by generating crosses of cultivated tomatoes with their wild relatives. Specific allele introgression relying on meiotic recombination, hampered structural divergence between parental genomes. However, previous studies interspecific tomato hybridization focused single cross or lacked resolution...
Abstract Interactions occurring between DNA and proteins across the nuclear genome regulate numerous processes, including meiosis. Meiosis ensures genetic variation balanced segregation of homologous chromosomes. It involves complex DNA‐protein interactions entire to a broad range formation repair double‐strand breaks (DSBs), chromosome compaction, homolog pairing, synapsis, recombination. The latter meiotic event, recombination, often occurs at discrete locations in genome, within tight...
Chouaref et al., (2019). A reproducible Snakemake pipeline to analyse Illumina paired-end data from ChiP-Seq experiments. Journal of Open Source Software, 4(38), 1465, https://doi.org/10.21105/joss.01465