A5028866825- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Chemokine receptors and signaling
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antimicrobial Peptides and Activities
- Cancer, Stress, Anesthesia, and Immune Response
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Nitric Oxide and Endothelin Effects
- HIV Research and Treatment
- Virus-based gene therapy research
- Cancer Research and Treatments
- Immunodeficiency and Autoimmune Disorders
- Cardiac Ischemia and Reperfusion
- Histone Deacetylase Inhibitors Research
- Adrenal Hormones and Disorders
- Systemic Lupus Erythematosus Research
- Reproductive System and Pregnancy
- Venous Thromboembolism Diagnosis and Management
- Diverse Scientific Research Studies
- Helicobacter pylori-related gastroenterology studies
- Advanced Glycation End Products research
- CAR-T cell therapy research
University of Buenos Aires
2013
Fondazione Humanitas per la Ricerca
2007-2008
IRCCS Humanitas Research Hospital
2008
Humanitas University
2008
University of Brescia
2007
Academia Nacional de Medicina
1993-2007
Mario Negri Institute for Pharmacological Research
2001-2005
European Institute of Oncology
2005
National Institute of Allergy and Infectious Diseases
2005
National Institutes of Health
2005
Dendritic cells (DCs) and macrophages are professional antigen-presenting (APCs) that play key roles in both innate adaptive immunity. ChemR23 is an orphan G protein-coupled receptor related to chemokine receptors, which expressed specifically these cell types. Here we present the characterization of chemerin, a novel chemoattractant protein, acts through abundant diverse set human inflammatory fluids. Chemerin secreted as precursor low biological activity, upon proteolytic cleavage its...
Abstract 1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) is an immunomodulatory agent inducing dendritic cells (DCs) to become tolerogenic. To further understand its mechanisms of action, we have examined the effects 1,25(OH)2D3 on tolerogenic properties blood myeloid (M-DCs) and plasmacytoid (P-DCs) human DC subsets. Exposure M-DCs up-regulated production CCL22, a chemokine attracting regulatory T cells, whereas CCL17, other CCR4 ligand, was reduced. also decreased IL-12p75 by M-DCs, as expected,...
Macrophage-derived chemokine (MDC)/CCL22 is a CC active on dendritic cells (DC), NK and Th2 lymphocytes. The present study was aimed at comprehensively investigating MDC production in vitro vivo. DC were the most potent producers of among leukocytes tested. Endothelial did not produce under variety conditions. Signals that induce maturation (lipopolysaccharide, IL-1, TNF, CD40 ligand, recognition bacteria yeast) dramatically augmented production, dexamethasone vitamin D3 blocked it....
Abstract To examine the different roles of myeloid dendritic cells (M-DCs) and plasmacytoid (P-DCs) in induction regulation immune response, we have studied chemokine secretion by freshly isolated DC subsets response to bacterial, viral, T cell-derived stimuli. M-DCs selectively produced very high levels homeostatic chemokines CC ligand (CCL)17 CCL22, while P-DCs little if any. In contrast, proinflammatory CCL3 was secreted mostly P-DCs, whereas CCL4 CXC 8 were both subsets. The selective...
Abstract Dendritic cells (DC) orchestrate the trafficking of lymphocytes by secreting chemokines with different specificity and function. Chemokines are produced at higher levels mature DC. This study shows that CCL18 is one most abundant immature In contrast to all other investigated date, was selectively down-regulated during maturation process induced LPS, TNF, CD40 ligand, Staphylococcus aureus Cowan I, Candida albicans, influenza virus. IL-10 vitamin D3, two known inhibitors DC...
The promiscuous D6 receptor binds several inflammatory CC chemokines and has been recently proposed to act as a chemokine-scavenging decoy receptor. present study was designed better characterize the spectrum of scavenged by D6, focusing in particular on CCR4 ligands analyzing influence NH(2)-terminal processing recognition this Using transfectants, it found that efficiently bound most (CCR1 through CCR5 agonists). Homeostatic (CCR6 CCR7 agonists) were not recognized D6. agonists chemokine...
Abstract Activation of NADPH oxidase represents an essential mechanism defense against pathogens. Dendritic cells (DC) are phagocytic specialized in Ag presentation rather than bacteria killing. Human monocyte-derived DC were found to express the components and release superoxide anions response phorbol esters agonists. The p47phox gp91phox down-regulated during monocyte differentiation DC, maturation with pathogen-derived molecules, known activate TLRs, increased expression enhanced...
Abstract Histone deacetylase (HDAC) inhibitors are small molecules inducing cell-cycle arrest, differentiation, and apoptosis, currently undergoing clinical trials as anticancer drugs. In addition, emerging evidence suggests HDAC may have anti-inflammatory immunomodulatory properties well, although the molecular mechanisms remain poorly defined. Given central role of dendritic cells (DC) in induction maintenance inflammatory immune response, we investigated effects on maturation activation...
A bstract : Dendritic cells (DCs) comprise a family of specializing in antigen capture and presentation to T cells. We have recently shown that DC play an active role bacterial uptake across mucosal surfaces. Indeed, are able open tight junctions sample antigens directly epithelia, both vitro vivo . Because express junction proteins, the integrity epithelial barrier is preserved. In this study we analyzed possible involvement controlling function. developed model our laboratory consisting...
Abstract The interaction between immune complexes (IC) and the receptors for Fc portion of IgG (FcγRs) triggers regulatory effector functions in system. In this study, we investigated effects IC on differentiation, maturation, human monocyte-derived dendritic cells (DC). When were added day 0, DC generated 6 (IC-DC) showed lower levels CD1a increased expression CD14, MHC class II, macrophage marker CD68, as compared with normally differentiated DC. use specific blocking FcγR mAbs indicated...
SUMMARY Endotoxin or lipopolysaccharide (LPS) tolerance may be partially due to the secretion of potent anti-inflammatory cytokines following severe Gram-negative infections, by low doses LPS. In this work, we describe effects interleukin-1β (IL-1β) and tumour necrosis factor alpha (TNF-α), two early secreted after LPS exposure, in induction tolerance. Our results demonstrate that mice treated with three daily 100 ng IL-1β were tolerant LPS-induced shock. However, TNF-α was unable induce an...
Abstract During Gram-negative infections bacterial components, such as LPS and formylated peptides, exert profound physiological effects on polymorphonuclear neutrophils (PMN) resulting in increased neutrophil effector activities, including the generation of oxidative metabolites, degranulation, phagocytosis cytokine release. There is not enough evidence about relationships between peptides triggering regulation immune inflammatory response. In this study, we present indicating that...