A5006530264- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Extracellular vesicles in disease
- Organ Transplantation Techniques and Outcomes
- Circular RNAs in diseases
- Immune Cell Function and Interaction
- Transplantation: Methods and Outcomes
- Antibiotic Resistance in Bacteria
- COVID-19 Clinical Research Studies
- Cancer-related molecular mechanisms research
- Tissue Engineering and Regenerative Medicine
- interferon and immune responses
- GDF15 and Related Biomarkers
- Phagocytosis and Immune Regulation
- Urban Planning and Valuation
- Neuroblastoma Research and Treatments
- Inflammasome and immune disorders
- Diabetic Foot Ulcer Assessment and Management
- SARS-CoV-2 and COVID-19 Research
- RNA Research and Splicing
- Heme Oxygenase-1 and Carbon Monoxide
- Long-Term Effects of COVID-19
- Congenital Anomalies and Fetal Surgery
- Bone Tumor Diagnosis and Treatments
- Cell Adhesion Molecules Research
Ri.MED
2017-2025
Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione
2021-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2022-2024
Human mesenchymal stromal cells (MSCs) represent the prototype in regenerative medicine, and are most widely studied cell type both preclinical clinical trials. It has been demonstrated that MSCs directly affect immune response by expressing soluble immunosuppressive factors such as IDO, PGE2 IL-10. also release exosomes able to give different effects, a paracrine manner, influencing inflammatory processes. The effects of on responses suggested their direct interaction with almost all...
Since the breakout of COVID-19, mutated forms severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have shown enhanced rates transmission and adaptation to humans. The variants concern (VOC), designated Alpha, Beta, Gamma, Delta, Omicron emerged independent one another, in turn rapidly became dominant. success each VOC, as well virus fitness, were enabled by altered intrinsic functional properties and, reasonably, antigenicity changes, conferring ability evade a primed immune...
Currently, Klebsiella pneumoniae is a pathogen of clinical relevance due to its plastic ability acquiring resistance genes multiple antibiotics. During K. infections, lipopolysaccharides (LPS) play an ambiguous role as they both activate immune responses but can also in evasion. The LPS O2a and O2afg serotypes are prevalent most multidrug resistant strains. Thus, we sought understand if those two particular were involved mechanism We have extracted (serotypes O1, O2afg) from strains and,...
Mesenchymal stromal/stem cells (MSCs) are believed to function in vivo as a homeostatic tool that shows therapeutic properties for tissue repair/regeneration. Conventionally, these expanded two-dimensional (2D) cultures, and, case, MSCs undergo genotypic/phenotypic changes resulting loss of their capabilities. Moreover, several clinical trials using have shown controversial results with moderate/insufficient responses. Different priming methods were tested improve MSC effects, and...
Mesenchymal stromal/stem cells (MSCs) are a heterogeneous population of multipotent that can be obtained from various tissues, such as dental pulp, adipose tissue, bone marrow and placenta. MSCs have gained importance in the field regenerative medicine because their promising role cell therapy regulatory abilities tissue repair regeneration. However, better characterization these products is necessary to further potentiate clinical application. In this study, we used unbiased high-resolution...
Highlights•Immune checkpoints may be involved in the onset of lung graft dysfunction•Dysregulation miRNAs impact expression immune checkpoints•Evaluation before transplantation could offer prognostic benefitsAbstractLung (LTx) significantly improves outcomes for patients with end-stage respiratory failure. However, primary dysfunction (PGD) remains one most relevant hurdles. Although PGD is attributed to ischemia-reperfusion injury (IRI), responses, primarily T cell-mediated, play a pivotal...
The clinical results of lung transplantation (LTx) are still less favorable than other solid organ transplants in both the early and long term. fragility lungs limits procurement rate can favor occurrence ischemia-reperfusion injury (IRI). Ex vivo perfusion (EVLP) with Steen SolutionTM (SS) aims to address problems, implementation EVLP alleviate activation IRI-mediated processes has been achieved using mesenchymal stromal/stem cell (MSC)-based treatments. In this study, we investigated...
Mesenchymal stromal/stem cells (MSCs) have emerged as a therapeutic tool in regenerative medicine. Recent studies shown that exosome (EXO)-derived microRNAs (miRNAs) play crucial role mediating MSC functions. Additionally, intracellular miRNAs been found to regulate capacities. However, the molecular mechanisms underlying miRNA-mediated effects are not fully understood. We used 3D culture and IFN-γ prime/enhance terms of functional miRNAs. After priming, our analysis revealed stable...
Abstract Proteolytic release of transmembrane proteins from the cell surface, so called ectodomain shedding, is a key process in inflammation. Inactive rhomboid 2 (iRhom2) plays crucial role this context, that it guides maturation and function sheddase ADAM17 (a disintegrin metalloproteinase 17) immune cells, and, ultimately, its ability to inflammatory mediators such as tumor necrosis factor α (TNFα). Yet, macrophage sheddome iRhom2/ADAM17, which collection substrates are released by...
Despite low levels of vascular endothelial growth factor (VEGF)-A, the secretome human Wharton’s jelly (WJ) mesenchymal stromal cells (MSCs) effectively promoted proangiogenic responses in vitro, which were impaired upon depletion small (~140 nm) extracellular vesicles (EVs). The isolated EVs shared VEGF-A profile and expressed five microRNAs, upregulated compared to fetal dermal MSC-derived EVs. These microRNAs exclusively targeted gene within 54 Gene Ontology (GO) biological processes, 18...
Ischemia/reperfusion injury (IRI) represents one of the leading causes primary non-function acute liver transplantation failure. IRI, generated by an interruption organ blood flow and subsequent restoration upon transplant, i.e., reperfusion, generates activation inflammatory cascade from resident Kupffer cells, first to neutrophils recruitment second apoptosis parenchyma. Recently, human mesenchymal stromal/stem cells (hMSCs) derivatives have been implemented for reducing damage induced...
Abstract Cigarette smoking impairs the lung innate immune response making smokers more susceptible to infections and severe symptoms. Dysregulation of cell death is emerging as a key player in chronic inflammatory conditions. We have recently reported that short exposure human monocyte-derived macrophages (hMDMs) cigarette smoke extract (CSE) altered TLR4-dependent lipopolysaccharide (LPS). CSE caused inhibition MyD88-dependent activation TRIF/caspase-8/caspase-1 pathway leading Gasdermin D...
One of the goals personalized medicine is to understand and treat diseases with greater precision through molecular profile patient. This profiling becoming a powerful tool for discovery novel biomarkers that can guide physicians in assessing, advance, disease stage, monitoring progression. Circulating miRNAs exosomal miRNAs, group small non-coding RNAs, are considered gold standard diagnostic human diseases. We have previously demonstrated osteosarcoma-derived exosomes able influence...
Natural killer (NK) cells mount an immune response against hepatitis C virus (HCV) infection and can be activated by several cytokines, including interleukin-2 (IL-2), IL-15, interferon-alpha (IFN-α). By exploiting the Huh7.5 hepatoma cell line infected with HCV JFH1 genome, we provide novel insights into antiviral effector functions of human primary NK after cytokine stimulation. IFN-α (IFNα-NKs) had enhanced contact-dependent -independent responses as compared IL-2/IL-15 (IL2/IL15-NKs)...
There is a need to improve acute respiratory distress syndrome (ARDS) diagnosis and management, particularly with extracorporeal membrane oxygenation (ECMO), different biomarkers have been tested implement precision-focused approach. We included ARDS patients on veno-venous (V-V) ECMO in prospective observational pilot study. Blood samples were obtained before cannulation, screened for the expression of 754 circulating microRNA (miRNAs) using high-throughput qPCR hierarchical cluster...
This preclinical proof-of-concept study aimed to evaluate the effectiveness of secretome therapy in diabetic mice with pressure ulcers. We utilized a custom-made hyaluronic acid (HA)-based porous sponge, which was rehydrated either normal culture medium or derived from human mesenchymal stromal cells (MSCs) achieve hydrogel consistency. Following application onto skin ulcers, both hydrogel-only and + combination accelerated wound closure compared vehicle group. Notably, presence...
Since its identification, HCV has been considered one of the main causes hepatitis and liver cancer. Currently, molecular mechanisms HCC development induced by infection have not sufficiently clarified. The recent discovery novel treatments that inhibit replication gave rise to new questions concerning mechanisms. In particular, eradication mediated direct-acting antiviral (DAAs) drugs does exclude possibility de novo development; this finding opened more on interplay between cells virus....
Chondrosarcoma is the second most common bone tumor, accounting for 20% of all cases. Little known about pathology and molecular mechanisms involved in development metastatic process chondrosarcoma. As a consequence, there are no approved therapies this tumor surgical resection only treatment currently available. Moreover, available biomarkers type chondrosarcoma classification relies on operator-dependent histopathological assessment. Reliable urgently needed, as well greater understanding...
The use of cell secreted factors in clinical settings could be an alternative to conventional therapy, with the advantage limiting concerns generally associated traditional transplantation, such as tumorigenicity, immunoreactivity, and carrying infections. Based on our published data, we predict a potential role for extracellular vesicles (EVs) contributing proangiogenic activity human fetal dermal secretome. Depletion nanosized EVs from secretome significantly impaired its ability induce...
Abstract In developed countries, cardiovascular diseases are currently the first cause of death. Cardiospheres (CSs) and cardiosphere‐derived cells (CDCs) have been found to ability regenerate myocardium after myocardial infarction (MI). recent years, much effort has made gain insight into human heart repair mechanisms, in which miRNAs shown play an important role. this regard, elucidate involvement miRNAs, we evaluated miRNA expression profile across biopsy, CSs CDCs using microarray...
<title>Abstract</title> <bold>Background </bold>Acute respiratory distress syndrome (ARDS) is a clinical definition of inflammatory lung disease comprising heterogeneous group causes. Despite the fact that veno-venous extracorporeal membrane oxygenation (V-V ECMO) may efficaciously support most severe cases ARDS, mortality still high. There need to improve both ARDS diagnosis and management, particularly with ECMO, different biomarkers have been tested implement precision-focused approach....
Spontaneous bacterial peritonitis (SBP) is a severe complication in patients with decompensated liver cirrhosis and commonly treated broad spectrum antibiotics. However, the rise of antibiotic resistance requires alternative therapeutic strategies. As recently shown, human amnion-derived mesenchymal stem cells (hA-MSCs) are able, vitro, to promote clearance modulate immune inflammatory response SBP. Our results highlight upregulation FOXO1, CXCL5, CXCL6, CCL20, MAPK13 hA-MSCs as well...