A5072211896- Mesenchymal stem cell research
- Cytomegalovirus and herpesvirus research
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Organ Transplantation Techniques and Outcomes
- Extracellular vesicles in disease
- Antibiotic Resistance in Bacteria
- HIV Research and Treatment
- Tissue Engineering and Regenerative Medicine
- Immunotherapy and Immune Responses
- Viral-associated cancers and disorders
- Pancreatic function and diabetes
- Viral Infections and Immunology Research
- Bacterial Identification and Susceptibility Testing
- Liver physiology and pathology
- Hepatitis B Virus Studies
- Mycobacterium research and diagnosis
- Liver Disease Diagnosis and Treatment
- Herpesvirus Infections and Treatments
- Renal Transplantation Outcomes and Treatments
- Hepatitis C virus research
- Cancer-related molecular mechanisms research
- Parvovirus B19 Infection Studies
- COVID-19 Clinical Research Studies
- Renal Diseases and Glomerulopathies
Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2015-2024
Ri.MED
2011-2021
University of Palermo
2020
Society for Healthcare Epidemiology of America
2019
University of Insubria
1998-2018
Clinica Luganese Moncucco
2014-2015
University of Pittsburgh Medical Center
2008
Ospedale di Circolo di Busto Arsizio
2002
University of Pavia
1993-1999
Donor-derived infections due to multidrug-resistant bacteria are a growing problem in solid organ transplantation, and optimal management options not clear. In 2-year period, 30/214 (14%) recipients received an from 18/170 (10.5%) deceased donors with infection or colonization caused by carbapenem-resistant gram-negative that was unknown at the time of transplantation. Among them, 14/30 (47%) transplant donor bacteremia infection/colonization transplanted were considered high risk...
Abstract Bone microenvironment provides growth and survival signals essential for osteosarcoma (OS) initiation progression. OS cells regulate communications inside tumor through different ways and, among all, tumor-derived exosomes support cancer progression metastasis. To define the contribution of OS-derived microenvironment, we investigated effects induced in bone remodeling mechanism angiogenesis. We demonstrated that promoted osteoclasts differentiation resorption activity. Furthermore,...
Human mesenchymal stromal cells (MSCs) represent the prototype in regenerative medicine, and are most widely studied cell type both preclinical clinical trials. It has been demonstrated that MSCs directly affect immune response by expressing soluble immunosuppressive factors such as IDO, PGE2 IL-10. also release exosomes able to give different effects, a paracrine manner, influencing inflammatory processes. The effects of on responses suggested their direct interaction with almost all...
The secretion of potential therapeutic factors by mesenchymal stem cells (MSCs) has aroused much interest given the benefits that it can bring in field regenerative medicine. Indeed, vitro multipotency these and secretive capacity both angiogenic immunomodulatory suggest a role tissue repair regeneration. However, during culture, MSCs rapidly lose expression key transcription associated with self-renewal, as well ability to produce functional paracrine factors. In our study, we show...
Since the breakout of COVID-19, mutated forms severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have shown enhanced rates transmission and adaptation to humans. The variants concern (VOC), designated Alpha, Beta, Gamma, Delta, Omicron emerged independent one another, in turn rapidly became dominant. success each VOC, as well virus fitness, were enabled by altered intrinsic functional properties and, reasonably, antigenicity changes, conferring ability evade a primed immune...
HIV-infected patients suffer several renal syndromes, which can progress rapidly from insufficiency to end-stage disease. Histologically, HIV-induced nephropathy is characterized by prominent tubulopathy with apoptosis of tubular cells. Clinical and experimental evidence suggests that injury may be directly related virus infection. Although HIV-1 a polytropic not solely lymphotropic pathogen, the susceptibility cells remains determined. This paper demonstrates in vitro permissiveness...
Fas–Fas ligand interaction is thought to be a crucial mechanism in controlling lymphocyte expansion by inducing apoptosis. However, Fas also broadly expressed on nonlymphoid cells, where its function vivo remains determined. In this study, we describe the development of inflammatory angiogenesis induced agonistic anti-Fas mAb Jo2 murine model Matrigel used as vehicle for delivery mediators. The subcutaneous implants mice containing became rapidly infiltrated endothelial cells and scattered...
This study reports on the characterization of two ceftazidime-avibactam (CZA)-resistant KPC-producing Klebsiella pneumoniae strains (KP-14159 and KP-8788) sequentially isolated from infections occurred in a patient never treated with CZA. Whole-genome sequencing using combined short- long-read approach showed that both isolates belonged to same ST258 strain, had altered outer membrane porins (a truncated OmpK35 an Asp137Thr138 duplication L3 loop OmpK36), carried novel pKpQIL plasmid...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes disease 2019 (COVID-19), is modifying human activity all over the world with significant health and economic burden. The advent of SARS-CoV-2 pandemic prompted scientific community to learn virus dynamics concerning transmissibility, epidemiology, usefulness vaccines in fighting emerging hazards. Pieces evidence suggest that first second doses mRNA induce a antibody response vaccinated subjects or patients who...
Donor-derived infections (DDI) sustained by carbapenem-resistant gram-negative bacteria (CR-GNB) in solid organ transplant recipients are potentially life-threatening. In this prospective study, we evaluate the incidence, factors associated with transmission, and outcome of unexpected CR-GNB DDI after implementation our local active surveillance system (LASS). LASS provides for early detection donor infections, prophylaxis at high-risk, diagnosis treatment DDI. Whole Genome Sequencing...
Mesenchymal stromal/stem cells (MSCs) are a heterogeneous population of multipotent that can be obtained from various tissues, such as dental pulp, adipose tissue, bone marrow and placenta. MSCs have gained importance in the field regenerative medicine because their promising role cell therapy regulatory abilities tissue repair regeneration. However, better characterization these products is necessary to further potentiate clinical application. In this study, we used unbiased high-resolution...
Abstract Virtually pure primary cultures of normal mammary epithelial cells (MEC) obtained from healthy women were shown to release interleukin 6 and 8 (IL6, IL8) produce a non‐secreted form tumor‐necrosis factor (TNF). No interferon (IFN), whether α, β or γ, ILI‐α ‐β could be detected. Analysis cellular RNA confirmed these findings showed that MEC also express IL6 receptor TNF‐α‐related mRNAs. Epithelial selectively stained by antibodies IL6, IL8 TNF‐α both in the gland. Samples human milk...
Although hepatic cell transplantation (CT) holds the promise of bridging patients with end-stage chronic liver failure to whole transplantation, suitable populations are under debate. In addition cells, mesenchymal stem cells (MSCs) and hematopoietic (HSCs) being considered as alternative sources for initial clinical work. Fetal (FL) tissue contains potential progenitors all these lineages. Based on collagenase incubation fragments, traditional isolation techniques yield only a fraction...
The role Hsp60 might play in various inflammatory and autoimmune diseases is under investigation, but little information exists pertaining to Hashimoto’s thyroiditis (HT). With the aim fill this gap, present work, we directed our attention participation HT pathogenesis. We found levels increased blood of patients compared controls. chaperonin was immunolocalized thyroid tissue specimens from with HT, both thyrocytes oncocytes (Hurthle cells) higher controls (goiter). In oncocytes, not only...
Cardiac progenitors, such as cardiospheres and cardiosphere-derived cells, represent an attractive cell source for cardiac regeneration. The PIWI-interacting RNAs, piRNAs, are intriguing class of small non-coding implicated in the regulation epigenetic state, maintenance genomic integrity stem functions. Although RNAs exploiting field cardiovascular research, piRNA signatures progenitors has not been evaluated yet.We profiled, through microarrays, 15,311 piRNAs expressed cardiospheres, cells...
The human EBV-transformed lymphoblastoid cell line (LCL), obtained by infecting peripheral blood monocular cells with Epstein–Barr Virus, has been extensively used for genetic, pharmacogenomic, and immunologic studies. Recently, the role of exosomes also indicated as crucial in crosstalk between EBV host microenvironment. Because that LCL exosomal cargo might play maintaining persistent infection, since little is known regarding non-coding RNAs LCL, aim our work was comprehensive...
Human amniotic mesenchymal stromal cells (hAMSCs) have unique immunomodulatory properties making them attractive candidates for regenerative applications in inflammatory diseases. Most of their beneficial are mediated through secretome. The bioactive factors concurring to its therapeutic activity still unknown. Evidence suggests synergy between the two main components secretome, soluble and vesicular fractions, pivotal shifting inflammation promoting self-healing. Biological variability...