A5073730929- MicroRNA in disease regulation
- Ultrasound and Hyperthermia Applications
- Bone health and treatments
- Bone Metabolism and Diseases
- Circular RNAs in diseases
- Synthesis and biological activity
- Bone Tissue Engineering Materials
- Retinoids in leukemia and cellular processes
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Bone health and osteoporosis research
- Curcumin's Biomedical Applications
- Osteoarthritis Treatment and Mechanisms
- Cancer Cells and Metastasis
- Bone fractures and treatments
- Cancer-related molecular mechanisms research
- NF-κB Signaling Pathways
- 3D Printing in Biomedical Research
- Cancer Genomics and Diagnostics
- S100 Proteins and Annexins
- Synthesis and Characterization of Heterocyclic Compounds
- Thyroid and Parathyroid Surgery
- Ultrasound Imaging and Elastography
- Synthesis of Tetrazole Derivatives
- Pluripotent Stem Cells Research
Istituto Ortopedico Rizzoli
2015-2023
Istituti di Ricovero e Cura a Carattere Scientifico
2018
University of Palermo
2005-2015
Abstract Bone microenvironment provides growth and survival signals essential for osteosarcoma (OS) initiation progression. OS cells regulate communications inside tumor through different ways and, among all, tumor-derived exosomes support cancer progression metastasis. To define the contribution of OS-derived microenvironment, we investigated effects induced in bone remodeling mechanism angiogenesis. We demonstrated that promoted osteoclasts differentiation resorption activity. Furthermore,...
BRAF V600E is the most common mutation found in papillary thyroid carcinoma (PTC). Tissue inhibitor of metalloproteinases (TIMP-1) and nuclear factor (NF)-κB have been shown to play an important role cancer. In particular, TIMP-1 binds its receptor CD63 on cell surface membrane activates Akt signaling pathway, which eventually responsible for anti-apoptotic activity. The aim our study was evaluate whether interplay among these three factors exists exerts a functional PTCs. To this purpose,...
Anaplastic thyroid carcinoma (ATC) is a rare and aggressive endocrine tumor with highly undifferentiated morphology. It has been suggested that cancer stem cells (CSCs) might play central role in ATC. The objectives of this study were (i) to characterize CSCs from ex vivo ATC specimens by investigating the expression several pluripotent cell markers, (ii) evaluate vitro drug resistance modifications after specific CSC transcription factor switch-off.In experiments, eight formalin-fixed,...
Osteosarcoma (OS) is the most common primary bone sarcoma affecting life of pediatric patients. The clinical treatment faces numerous difficulties, including adverse effects chemotherapies, chemoresistance, and recurrences. In this study, resveratrol (RSV), a natural polyphenol, on OS cell lines were investigated to evaluate its action as an adjuvant therapy current chemotherapy regimens. RSV exhibited multiple tumor-suppressing activities lines, inducing series critical events. We found (1)...
We examined the effects of curcumin and its isoxazole analogue MR 39 in MCF-7 breast cancer cell line multidrug-resistant (MDR) variant MCF-7R. In comparison with MCF-7, MCF-7R lacks estrogen receptor α (ERα) overexpressess P-glycoprotein (P-gp), different IAPs (inhibitory apoptosis proteins) COX-2. Through analyses on proliferation, cycling death, we have observed that antitumor activity more potent (approximately two-fold) is at least equal MDR compared to parental MCF-7. Similar results...
Low‐intensity pulsed ultrasound (LIPUS) as an adjuvant therapy in vitro and vivo bone engineering has proven to be extremely useful. The present study aimed at investigating the effect of 30 mW/cm 2 LIPUS stimulation on commercially available human mesenchymal stem cells (hMSCs) cultured basal or osteogenic medium different experimental time points (7, 14, 21 days). hypothesis was that would improve differentiation hMSC guarantying maintenance committed fraction, demonstrated by cell...
Bone disease severely affects the quality of life over 70% multiple myeloma (MM) patients, which daily experience pain, pathological fractures, mobility issues and an increased mortality. Recent data have highlighted crucial role endoplasmic reticulum-associated unfolded protein response (UPR) in malignant transformation tumor progression; therefore, targeting UPR-related molecules may open novel therapeutic avenues. Endoplasmic reticulum (ER) stress UPR pathways are constitutively activated...
Bone tissue engineering is helpful in finding alternatives to overcome surgery limitations. growth and repair are under the control of biochemical mechanical signals; therefore, recent years several approaches improve bone regeneration have been evaluated. Osteo-inductive biomaterials, stem cells, specific factors biophysical stimuli among those. The aim present study was evaluate if low-intensity pulsed ultrasound stimulation (LIPUS) treatment would colonization an MgHA/Coll hybrid...
We tested the novel NF-kappaB inhibitor dehydroxymethylepoxyquinomicin (DHMEQ) in hepatic cancer (HCC) HepG2, HA22T/VGH and HuH-6 cells. The sensitivity to cell growth inhibitory apoptotic effects of agent increased along with levels constitutively activated NF-kappaB, which were low HepG2 higher HuH-6. In HA22T/VGH, DHMEQ exhibited synergy cisplatin. same cells, exerted dose-dependent decreases nuclear attenuated activation by It down-regulated Bcl-XL mRNA a manner up-regulated that Bcl-XS....
Bone tissue is one of the main sites for breast metastasis; patients diagnosed with advanced cancer mostly develop bone metastasis characterized by severe osteolytic lesions, which heavily influence their life quality. Low Intensity Pulsed Ultrasound (LIPUS) a form mechanical energy able to modulate various molecular pathways both in and health cells. The purpose present study was evaluate first time, ability LIPUS capability cells.Two different approaches were employed: a) Indirect method...
The roles of low-intensity pulsed ultrasound (LIPUS) and microRNAs (miRNAs) on hMSCs commitments have already been investigated; however, the effects application their co-treatments in an vitro cell model are still unknown. Our previous studies demonstrated that (i) LIPUS modulated cytoskeletal organization (ii) miRNA-675-5p a role HIF-1α signaling modulation during osteoblast commitment. We investigated for first time as promoter tool miRNA expression. Thanks to bioinformatic analysis, we...