A5007016584- Clay minerals and soil interactions
- Stress Responses and Cortisol
- NF-κB Signaling Pathways
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Neuroendocrine regulation and behavior
- Therapeutic Uses of Natural Elements
- Fungal and yeast genetics research
- Protein Kinase Regulation and GTPase Signaling
- Estrogen and related hormone effects
- Reproductive System and Pregnancy
- Mast cells and histamine
- Circadian rhythm and melatonin
- RNA and protein synthesis mechanisms
- Oral and gingival health research
- Cytokine Signaling Pathways and Interactions
- PI3K/AKT/mTOR signaling in cancer
- Memory and Neural Mechanisms
- Adipose Tissue and Metabolism
- RNA regulation and disease
- Photosynthetic Processes and Mechanisms
- Layered Double Hydroxides Synthesis and Applications
- Calcium signaling and nucleotide metabolism
- Receptor Mechanisms and Signaling
Institute for Biomedical Research and Innovation
2023-2025
National Research Council
2014-2025
Istituto di Biomedicina e di Immunologia Molecolare Alberto Monroy
2009-2019
University of Palermo
2017
Neurocentre Magendie
2005-2009
Inserm
2004-2009
Université de Bordeaux
2004-2009
Galen University
2005
Victor (Japan)
2005
University of Palermo
1989-2001
Compulsive drug-seeking behavior and its renewal in former drug addicts is promoted by several situations, among which reactivation of withdrawal memories plays a crucial role. A neural hypothesis that such reactivate the circuits involved itself promote motivational state leading to seeking or taking. To test this hypothesis, we have analyzed cell populations recruited when opiate-dependent rats are reexposed stimuli previously paired with (memory retrieval) compared them those underlying...
Abstract Glucocorticoid receptors (GRs) are transcription factors that, upon activation by glucocorticoids, translocate to the cell nucleus, and bind specific response elements (GREs) in promoter region of target genes. We analysed stress‐ circadian‐induced changes nuclear translocation GRE binding GRs hippocampus prefrontal cortex rat brain. Nuclear were measured extracts Western blot gel shift, respectively. When glucocorticoid levels low, as during light period circadian cycle, almost...
BRAF V600E is the most common mutation found in papillary thyroid carcinoma (PTC). Tissue inhibitor of metalloproteinases (TIMP-1) and nuclear factor (NF)-κB have been shown to play an important role cancer. In particular, TIMP-1 binds its receptor CD63 on cell surface membrane activates Akt signaling pathway, which eventually responsible for anti-apoptotic activity. The aim our study was evaluate whether interplay among these three factors exists exerts a functional PTCs. To this purpose,...
Supramolecular gel hybrids obtained by self-assembly of Fmoc-l-phenylalanine (Fmoc-F) in the presence functionalized halloysite nanotubes (f-HNT) were biocompatible solvents and employed as carriers for delivery camptothecin (CPT) molecules. The synthesis new f-HNT material well its characterization are described. properties hybrid hydrogels organogels analyzed several techniques. small amounts allows good dispersion tubes subsequent formation homogeneous gels. experimental results show that...
Fabry disease (FD) is an X-linked lysosomal storage disease, caused by mutations in the GLA gene on X chromosome, resulting a deficiency of enzyme α-GAL. This leads to progressive accumulation Gb3 cells, causing multi-systemic effects. FD has been classified as subgroup autoinflammatory diseases. NF-κB family ubiquitous and inducible transcription factors that play critical roles inflammation, which p65/p50 heterodimer most abundant. The glucocorticoid receptor (GR) represents physiological...
A novel carrier system based on halloysite nanotubes (HNT), for the potential intraarticular delivery of kartogenin (KGN) by means laponite (Lap) hydrogel (HNT/KGN/Lap), is developed. The drug was first loaded into HNT, and hybrid composite obtained used as filler hydrogel. Both were thoroughly investigated several techniques morphology imaged transmission electron microscopy. Furthermore, gelating ability in presence rheological properties also investigated. kinetic release from HNT HNT/Lap...
Several mechanisms are probably involved in determining the evolution of autoimmune thyroid disease (AITD) towards either hypothyroidism and clinical syndrome known as Hashimoto's thyroiditis (HT) or toward hyperthyroidism symptoms Graves' (GD). To gain further insight into such we performed an exhaustive comparative analysis expression key molecules regulating cell death (Fas, Fas ligand [FasL], Bcl-2) apoptosis both thyrocytes infiltrating lymphocytes (TILs) from patients with GD HT....
The Glucocorticoid Receptor (GR) is a transcription factor ubiquitously expressed in the brain. Activation of brain GRs by high levels glucocorticoid (GC) hormones modifies large variety physiological and pathological-related behaviors. Unfortunately specific cellular targets GR-mediated behavioral effects GC are still largely unknown. To address this issue, we generated mutated form GR called DeltaGR. DeltaGR constitutively transcriptionally active that localized nuclei activates without...
Ras proteins bind either GDP or GTP with high affinity. However, only the GTP‐bound form of yeast Ras2 protein is able to stimulate adenylyl cyclase. To identify amino acid residues that play a role in conversion from GDP‐bound state proteins, we have searched for single substitutions selectively affected binding one two nucleotides. We found replacement glycine‐82 by serine resulted an increased rate dissociation Gpp(NH)p, nonhydrolysable analog GTP, while was not significantly modified....
The use of open source technologies to create collaboration platforms can produce huge advantages with small investment.We set up a telemedicine network for healthcare district typical centralization issues developing countries. Our was built using broadband Internet connection, and the digital divide in rural areas reduced by means wireless connection. A software infrastructure deployed on implement platform among different facilities.We obtained an integrated modest investment hardware...
We have explored the role of distal switch II region yeast RAS2 protein in determining response to nucleotide exchange factor CDC25. first constructed tester strains which deletion chromosomal CDC25, RAS1, and genes, combination with suppressor CRI4, resulted detectable phenotypes vivo vitro. Phenotypes included impaired growth at 37 degrees C, defective glucose-induced cyclic AMP signaling, low adenylyl cyclase activity membrane preparations. Tester were subsequently used for reintroduction...
The existence of a biochemical network embryo-maternal communication implies that various secreted molecules constitute signal-response mechanism, important for the process embryo implantation in mammals. Here we report purification protein with an apparent molecular weight 136 kDa, responsible 2000-fold increase embryo-derived histamine-releasing factor (EHRF) activity. This protein, purified from medium in-vitro culture 2–8-cell human embryos, by means affinity chromatography, was capable...
Abstract The SCH9 yeast gene, that was previously identified as a suppressor of cdc25 and ras1 − ras2‐ts temperature‐sensitive mutants, encodes putative protein kinase positively regulates the progression cells through G1 phase cell cycle. We have determined structure transcription unit, using primer extension S1 mapping techniques. corresponding mRNA included an unusually long 5′ region more than 600 nucleotides preceding major open reading frame (ORF). While latter corresponded to 824...