A5057981252- Extracellular vesicles in disease
- Advanced Proteomics Techniques and Applications
- Chronic Myeloid Leukemia Treatments
- MicroRNA in disease regulation
- Chronic Lymphocytic Leukemia Research
- Immune cells in cancer
- Cancer, Hypoxia, and Metabolism
- Glycosylation and Glycoproteins Research
- Cancer, Lipids, and Metabolism
- Curcumin's Biomedical Applications
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Nanoplatforms for cancer theranostics
- Cell Adhesion Molecules Research
- Cancer Research and Treatments
- Ancient Mediterranean Archaeology and History
- Multiple Myeloma Research and Treatments
- Allergic Rhinitis and Sensitization
- Ferroptosis and cancer prognosis
- Food Allergy and Anaphylaxis Research
- Cancer Immunotherapy and Biomarkers
- Liver Disease Diagnosis and Treatment
- Fungal Biology and Applications
- Immunotherapy and Immune Responses
University of Palermo
2015-2024
University of Turin
2022-2024
Tecnologie Avanzate (Italy)
2003-2020
Ontario Institute for Cancer Research
2012
University Health Network
2012
University of Cagliari
2011
National Cancer Institute
2007-2008
Université Claude Bernard Lyon 1
2007
Centre National de la Recherche Scientifique
2007
Institut de Biologie et de Chimie des Protéines
2004-2007
The goal of this study was to understand if exosomes derived from high-metastatic cells may influence the behavior less aggressive cancer and properties endothelium. We found that metastatic colon are able transfer their amoeboid phenotype isogenic primary through exosomes, morphological transition is associated with acquisition a more behavior. Moreover, line (SW620Exos) exhibited higher ability cause endothelial hyperpermeability than non (SW480Exos). SWATH-based quantitative proteomic...
// Stefania Raimondo 1 , Flores Naselli Simona Fontana Francesca Monteleone Alessia Lo Dico Laura Saieva Giovanni Zito 2 Anna Flugy Mauro Manno 3 Maria Antonietta Di Bella Giacomo De Leo Riccardo Alessandro Dipartimento di Biopatologia e Biotecnologie Mediche, Università degli Studi Palermo, sezione Biologia Genetica, Italy Laboratorio Ingegneria Tissutale – Piattaforme Innovative per l’Ingegneria (PON01–00829), Istituto Ortopedico Rizzoli, Biofisica, Consiglio...
ABSTRACT Bioinformatics tools are imperative for the in depth analysis of heterogeneous high‐throughput data. Most software developed by specific laboratories or groups companies wherein they designed to perform required group. However, such may fail capture “what community needs a tool”. Here, we describe novel community‐driven approach build comprehensive functional enrichment tool. Using existing FunRich tool as template, invited researchers request additional features and/or changes....
Despite Imatinib (IM), a selective inhibitor of Bcr-Abl, having led to improved prognosis in Chronic Myeloid Leukemia (CML) patients, acquired resistance and long-term adverse effects is still being encountered.There is, therefore, urgent need develop alternative strategies overcome drug resistance.According the molecules expressed on their surface, exosomes can target specific cells.Exosomes also be loaded with variety molecules, thereby acting as vehicle for delivery therapeutic agents.In...
Chronic myeloid leukemia (CML) is a clonal hematopoietic stem cell disorder in which leukemic cells display reciprocal t(9:22) chromosomal translocation that results the formation of chimeric BCR-ABL oncoprotein, with constitutive tyrosine kinase activity. Consequently, causes increased proliferation, inhibition apoptosis, and altered adhesion blasts to bone marrow (BM) microenvironment. It has been well documented cancer can generate their own signals order sustain growth survival, recent...
// Lavinia Raimondi 1 , Angela De Luca Nicola Amodio 2 Mauro Manno 3 Samuele Raccosta Simona Taverna 4 Daniele Bellavia Flores Naselli Fontana Odessa Schillaci Roberto Giardino 5 Milena Fini 6 Pierfrancesco Tassone Alessandra Santoro 7 Giacomo Leo Gianluca Giavaresi 1,6 and Riccardo Alessandro 4,8 Laboratory of Tissue Engineering - Innovative Technology Platforms for (PON01-00829), Rizzoli Orthopedic Institute, Palermo, Italy Department Experimental Clinical Medicine, Magna Graecia...
Tumor derived exosomes are vesicles which contain proteins and microRNAs that mediate cell-cell communication involved in angiogenesis tumor progression. Curcumin from the plant Curcuma longa, shows anticancer effects. Exosomes released by CML cells treated with a high amount of miR-21 is shuttled into endothelial biologically active form. The treatment HUVECs Curcu-exosomes reduced RhoB expression negatively modulated motility. We showed addition control to caused an increase IL8 VCAM1...
Tumor-associated macrophages play a key role in promoting tumor progression by exerting an immunosuppressive phenotype associated with the expression of programmed cell death ligand 1 (PD-L1). It is well known that tumor-derived small extracellular vesicles (SEVs) affect microenvironment, influencing TAM behavior. The present study aimed to examine effect SEVs derived from colon cancer and multiple myeloma cells on macrophage functions. Non-polarized (M0) differentiated THP-1 were...
Abstract Dendritic cells (DCs) are essential orchestrators of immune responses and represent potential targets for immunomodulation in autoimmune diseases. Human amniotic fluid secretome is abundant immunoregulatory factors, with extracellular vesicles (EVs) being a significant component. However, the impact these EVs on dendritic subsets remain unexplored. In this study, we investigated interaction between highly purified cell derived from stem lines (HAFSC‐EVs). Our results suggest that...
Urinary expressed prostatic secretion or "EPS-urine" is proximal tissue fluid that collected after a digital rectal exam (DRE). EPS-urine rich source of prostate-derived proteins can be used for biomarker discovery prostate cancer (PCa) and other diseases. We previously conducted comprehensive proteome analysis direct secretions (EPS). In the current study, we defined employing Multidimensional Protein Identification Technology (MudPIT) providing catalogue this body future studies....
Solid tumors subjected to intermittent hypoxia are characterized by resistance chemotherapy and immune-killing effector T-lymphocytes, particularly tumor-infiltrating Vγ9Vδ2 T-lymphocytes. The molecular circuitries determining this double not known.We analyzed a panel of 28 human non-small cell lung cancer (NSCLC) lines, using an in vitro system simulating continuous hypoxia. Chemosensitivity cisplatin docetaxel was evaluated chemiluminescence, ex vivo T-lymphocyte expansion flow cytometry....
Low‐intensity pulsed ultrasound (LIPUS) as an adjuvant therapy in vitro and vivo bone engineering has proven to be extremely useful. The present study aimed at investigating the effect of 30 mW/cm 2 LIPUS stimulation on commercially available human mesenchymal stem cells (hMSCs) cultured basal or osteogenic medium different experimental time points (7, 14, 21 days). hypothesis was that would improve differentiation hMSC guarantying maintenance committed fraction, demonstrated by cell...
Bone disease severely affects the quality of life over 70% multiple myeloma (MM) patients, which daily experience pain, pathological fractures, mobility issues and an increased mortality. Recent data have highlighted crucial role endoplasmic reticulum-associated unfolded protein response (UPR) in malignant transformation tumor progression; therefore, targeting UPR-related molecules may open novel therapeutic avenues. Endoplasmic reticulum (ER) stress UPR pathways are constitutively activated...
In the last years, field of nanomedicine and drug delivery has grown exponentially, providing new platforms to carry therapeutic agents into target sites. Extracellular vesicles (EVs) are ready-to-use, biocompatible, non-toxic nanoparticles that revolutionizing delivery. EVs involved in cell–cell communication mediate many physiological pathological processes by transferring their bioactive cargo cells. Recently, nanovesicles from plants (PDNVs) raising interest scientific community due high...
Chronic myelogenous leukemia (CML) is a myeloproliferative disorder caused by expression of the chimeric BCR-ABL tyrosine kinase oncogene, resulting from t(9;22) chromosomal translocation. Imatinib (gleevec, STI-571) selective inhibitor activity highly effective in treatment CML. However, even though almost all CML patients respond to with imatinib or third generation inhibitors, these drugs are not curative and need be taken indefinitely until become resistant. Therefore, get definitive...
The combination of pemetrexed and cisplatin remains the reference first-line systemic therapy for malignant pleural mesothelioma (MPM). Its activity is moderate because tumor aggressiveness, immune-suppressive environment resistance to chemotherapy-induced immunogenic cell death (ICD). Preliminary limited findings suggest that MPM cells have deregulated ubiquitination proteasome activities, although inhibitors achieved disappointing clinical results.Here, we investigated role E3-ubiquitin...
Intercellular communication between cancer cells themselves or with healthy in the tumor microenvironment and/or pre-metastatic sites plays an important role progression and metastasis. In addition to ligand–receptor signaling complexes, extracellular vesicles (EVs) are emerging as novel mediators of intercellular both tissue homeostasis diseases such cancer. EV-mediated transfer molecular activities impacting morphological features cell motility from highly metastatic SW620 non-metastatic...
Glioblastoma multiforme (GBM) is an aggressive tumor, difficult to treat pharmacologically because of the blood-brain barrier (BBB), which rich in ATP-binding cassette (ABC) transporters and tight junction (TJ) proteins. The BBB disrupted within GBM bulk, but it competent brain-adjacent-to-tumor areas, where eventual foci can trigger tumor relapse. How cells influence permeability poorly investigated.