A5014999187- Cancer, Hypoxia, and Metabolism
- Occupational and environmental lung diseases
- Drug Transport and Resistance Mechanisms
- Nanoparticle-Based Drug Delivery
- Nitric Oxide and Endothelin Effects
- Nanoplatforms for cancer theranostics
- Cancer, Lipids, and Metabolism
- Aluminum toxicity and tolerance in plants and animals
- RNA modifications and cancer
- Nanoparticles: synthesis and applications
- Sarcoma Diagnosis and Treatment
- Histone Deacetylase Inhibitors Research
- Metal complexes synthesis and properties
- Metabolism and Genetic Disorders
- Photodynamic Therapy Research Studies
- Occupational exposure and asthma
- RNA Interference and Gene Delivery
- Electron Spin Resonance Studies
- Ferrocene Chemistry and Applications
- Graphene and Nanomaterials Applications
- Glioma Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- Heme Oxygenase-1 and Carbon Monoxide
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer therapeutics and mechanisms
University of Turin
2014-2023
Accademia Albertina delle Belle Arti
2022
Candiolo Cancer Institute
2013
Pediatrics and Genetics
2008
University of Bologna
2007
Diphenyleneiodonium (DPI) and the structurally related compound diphenyliodonium (DIP) are widely used as inhibitors of flavoenzymes, particularly NADPH oxidase. Here we report further evidence that DPI DIP not specific flavin binders. A 3-h incubation N11 glial cells with significantly inhibited in a dose-dependent way both pentose phosphate pathway tricarboxylic acid cycle. In parallel, observed increase reactive oxygen species generation lipoperoxidation increased leakage lactate...
Two samples of highly pure multiwalled carbon nanotubes (MWCNTs) similar in hydrophobicity and surface reactivity were prepared with length, <5 μm, but markedly different diameter (9.4 vs 70 nm). The compared for their cytotoxic activity, uptake, ability to induce oxidative stress (ROS production intracellular GSH depletion) vitro murine alveolar macrophages (MH-S). vivo toxicity was evaluated by measuring biochemical (LDH activity total proteins) cellular responses bronchoalveolar lavage...
Multidrug resistance (MDR) is a critical hindrance to the success of cancer chemotherapy. The main thing responsible for MDR phenotypes are plasma-membranes associated with adenosine triphosphate (ATP) Binding Cassette (ABC) drug efflux transporters, such as P-glycoprotein (Pgp) transporter that has broadest spectrum substrates. Curcumin (CURC) Pgp inhibitor, but it poorly soluble and bioavailable. To overcome these limitations, we validated efficacy safety CURC, loaded in biocompatible...
The activity of P-glycoprotein (Pgp) and multidrug resistance related protein 1 (MRP1), two membrane transporters involved in colon cancer, is increased by high amounts cholesterol plasma detergent resistant membranes (DRMs). It has never been investigated whether omega 3 polyunsatured fatty acids (PUFAs), which modulate homeostasis dyslipidemic syndromes have chemopreventive effects may affect the response to chemotherapy (MDR) tumors.We studied effect PUFAs docosahexaenoic acid (DHA)...
Nitric oxide (NO), a modulator of several physiological processes, is involved in different human sperm functions. We have investigated whether NO may stimulate the motility spermatozoa via activation soluble guanylate cyclase (sGC)/cGMP pathway. Sperm samples obtained by masturbation from 70 normozoospermic patients were processed swim-up technique. The kinetic parameters motile sperm-rich fractions assessed computer-assisted analysis. After 30-90 min incubation, donor S-nitrosoglutathione...
Lung toxicity mediated by multiwalled carbon nanotubes (MWCNT) has been widely demonstrated and recently associated with induction of carcinogenic asbestos-like effects, but the chemical features that drive this toxic effect have still not well elucidated. The presence metals as trace contaminants during MWCNT preparation, in particular iron (Fe) impurities, plays an important role determining a different cellular response to MWCNT. Our goal was clarify mechanisms underlying MWCNT-induced...
"Vitreous silica" is a particular form of amorphous silica, much neglected in experimental studies on silica toxicity. In spite the incorrect term "quartz glass", often employed, this material fully amorphous. When reduced powdered by grinding, particulate appears most close to workplace quartz dust but, opposite quartz, not crystalline. As silicosis and lung cancer are also found among workers exposed question arises whether crystallinity prerequisite feature that makes toxic. We compare...
Doxorubicin is one of the leading drugs for osteosarcoma standard chemotherapy. A total 40% to 45% high-grade patients are unresponsive, or only partially responsive, doxorubicin (Dox), due overexpression drug efflux transporter ABCB1/P-glycoprotein (Pgp). The aim this work improve Dox-based regimens in resistant osteosarcomas. We used a chemically modified mitochondria-targeted Dox (mtDox) against Pgp-overexpressing osteosarcomas with increased resistance Dox. Unlike Dox, mtDox accumulated...
Multi-walled carbon nanotubes (MWCNT) are currently under intense toxicological investigation due to concern on their potential health effects. Current in vitro and vivo data indicate that MWCNT exposure is strongly associated with lung toxicity (inflammation, fibrosis, granuloma, cancer airway injury) effects might be comparable asbestos-induced carcinogenesis. Although fibrosis a multi-origin disease, epithelial-mesenchymal transition (EMT) recently recognized as an important pathway cell...
Heme is an iron-containing porphyrin of vital importance for cell energetic metabolism. High rates heme synthesis are commonly observed in proliferating cells. Moreover, the cell-surface exporter feline leukemia virus subgroup C receptor 1a (FLVCR1a) overexpressed several tumor types. However, reasons why and export enhanced highly cells remain unknown. Here, we illustrate a functional axis between export: efflux through plasma membrane sustains synthesis, implementation two processes...
In the Italian western Alps, asbestos mineralization (both chrysotile and tremolite amphibole) takes place from serpentinites, together with other less common asbestiform minerals not regulated by current legislation. context of a study on evaluation risk in this area, possible role played associated overall toxicity airborne fraction has been examined. The first mineral investigated was balangeroite [(Mg,Fe2+,Fe3+,Mn2+)42Si16O54(OH)36], an iron-rich contaminant Balangero mine (Piedmont),...
The generation of reactive oxygen species and other radicals, catalyzed by iron ions at the fiber surface, is thought to play an important role in asbestos-induced cytotoxicity genotoxicity, but a direct confirmation this statement needs availability asbestos samples differing only for their content, without interference physicochemical features. Synthetic stoichiometric chrysotile nanofibers, devoid or any contaminant, did not exert genotoxic cytotoxic effects nor elicited oxidative stress...
Abstract Background Doxorubicin is one of the few chemotherapeutic drugs able to exert both cytotoxic and pro-immunogenic effects against cancer cells. Following drug administration, intracellular protein calreticulin translocated with an unknown mechanism onto plasma membrane, where it triggers phagocytosis tumour cells by dendritic Moreover doxorubicin up-regulates inducible nitric oxide (NO) synthase (iNOS) gene in cells, leading huge amounts NO, which turn acts as a mediator toxicity...
Arianna Maruccoab, Elena Gazzanobc, Dario Ghigobc, Emanuele Enricod & Ivana Fenoglioab*a Department of Chemistry and NIS – Nanostructured Interfaces Surfaces, University Torino, Italy, b "G. Scansetti" Interdepartmental Centre for Studies on Asbestos other Toxic Particulates, c Oncology, d Nanofacility Unit, Istituto Nazionale di Ricerca Metrologica (INRIM), Italy
Doxorubicin (DOXO) is one of the most effective antineoplastic agents in clinical practice. Its use limited by acute and chronic side effects, particular its cardiotoxicity rapid development resistance to it. As part a program aimed at developing new DOXO derivatives endowed with reduced cardiotoxicity, active against DOXO-resistant tumor cells, series H2S-releasing DOXOs (H2S-DOXOs) were obtained combining appropriate H2S donor substructures. The resulting compounds studied on H9c2...