A5008442794- Mesenchymal stem cell research
- Extracellular vesicles in disease
- Tissue Engineering and Regenerative Medicine
- Neonatal Respiratory Health Research
- MicroRNA in disease regulation
- Infant Nutrition and Health
- Periodontal Regeneration and Treatments
- Corneal Surgery and Treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Advanced biosensing and bioanalysis techniques
- Tendon Structure and Treatment
- Liver Disease Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Pancreatic function and diabetes
- Liver physiology and pathology
- Osteoarthritis Treatment and Mechanisms
- Muscle Physiology and Disorders
- Traumatic Brain Injury and Neurovascular Disturbances
- Hematopoietic Stem Cell Transplantation
Fondazione Poliambulanza Istituto Ospedaliero
2016-2025
Fondazione Santa Lucia
2023
Weatherford College
2023
Centro Ricerche FIAT
2023
To define the features of human amniotic mesenchymal stromal cell secretome and its protective properties in experimental models acute brain injury.Prospective study.Laboratory research.C57Bl/6 mice.Mice subjected to sham or traumatic injury by controlled cortical impact received cells phosphate-buffered saline infused intracerebroventricularly intravenously 24 hours after injury. Organotypic slices exposed ischemic oxygen-glucose deprivation were treated with their (conditioned medium) a...
Amniotic mesenchymal stromal cells (hAMSCs) have unique immunomodulatory properties demonstrated in vitro and vivo various diseases which the dysregulated immune system plays a major role. The pro-regenerative effects of MSCs, among hAMSCs lie bioactive factors they secrete their paracrine activity, is well known. mix these (i.e., secretome) can be either freely secreted or conveyed by extracellular vesicles (EV), thus identifying two components cell secretome: EV-free EV fractions. This...
Administration of horse amniotic mesenchymal cells (AMCs) and their conditioned medium (AMC-CM) improves the in vivo recovery spontaneous equine tendon lesions inhibits vitro proliferation peripheral blood mononuclear (PBMC). This process may involve microvesicles (MVs) as an integral component cell-to-cell communication during tissue regeneration. In this study, presence type MVs secreted by AMCs were investigated response to was studied using a dose–response curve at different...
Abstract Growing evidence suggests a mechanistic link between inflammation and the development progression of fibrotic processes. Mesenchymal stromal cells derived from human amniotic membrane (hAMSCs), which display marked immunomodulatory properties, have been shown to reduce bleomycin-induced lung fibrosis in mice, possibly by creating microenvironment able limit evolution chronic fibrosis. However, ability hAMSCs modulate immune involved pulmonary has yet be elucidated. Herein, we...
Human amniotic mesenchymal stromal cells (hAMSCs) have unique immunomodulatory properties making them attractive candidates for regenerative applications in inflammatory diseases. Most of their beneficial are mediated through secretome. The bioactive factors concurring to its therapeutic activity still unknown. Evidence suggests synergy between the two main components secretome, soluble and vesicular fractions, pivotal shifting inflammation promoting self-healing. Biological variability...
Abstract Myofibroblasts are key fibrogenic cells responsible for excessive extracellular matrix synthesis characterizing the fibrotic lesion. In liver fibrosis, myofibroblasts derive either from activation of hepatic stellate (HSC) and portal fibroblasts (PF), or that originate ductular epithelial undergoing epithelial–mesenchymal transition. Ductular can also indirectly promote myofibroblast generation by activating TGF‐β, main growth factor, through αvβ6 integrin. addition, after injury,...
Abstract Background It is now well established that factors (free or in extracellular vesicles) secreted by mesenchymal stromal cells (MSC) are important mediators of MSC regenerative actions. Herein we produced the secretome (conditioned medium, CM) from isolated amniotic membrane (hAMSC) and CM intact (hAM, no manipulation enzymatic digestion) order to potentially identify an effective, easy less expensive produce for potential applications medicine. Given immunomodulation a key mechanism...
Equine amniotic mesenchymal stromal cells (AMSCs) and their conditioned medium (CM) were evaluated for ability to inhibit in vitro proliferation of peripheral blood mononuclear (PBMCs) with without priming. Additionally, AMSC immunogenicity was assessed by expression MHCI MHCII counteract the inflammatory process.Horse PBMC induced phytohemagglutinin. priming performed 10 ng/ml TNF-α, 100 IFN-γ, a combination 5 TNF-α 50 IFN-γ. The CM generated from naïve unprimed primed AMSCs also tested...
Currently, more than 30 000 allogeneic hematopoietic stem cell (HSC) transplantations have been performed for the treatment of hematological and nonhematological diseases using HSC from umbilical cord blood (CB). However, wide utilization CB as a source is limited by low number cells recovered. One strategy to expand ex vivo CB-HSC represented use bone marrow mesenchymal stromal (BM-MSCs) feeder enhance proliferation while maintaining stemness. Indeed, BM-MSCs recognized one most relevant...
Abstract In the cell therapy scenario, efficient tracing of transplanted cells is essential for investigating migration and interactions with host tissues. This fundamental to provide mechanistic insights which altogether allow understanding translational potential placental in clinical setting. Mesenchymal stem/stromal ( MSC ) from human placenta are increasingly being investigated their treating patients a variety diseases. this study, we feasibility using poly (methyl methacrylate)...
Abstract Background Duchenne muscular dystrophy (DMD) is a muscle disease caused by mutations in the dystrophin gene characterized myofiber fragility and progressive degeneration. The genetic defect results reduced number of self-renewing satellite cells (MuSCs) an impairment their activation differentiation which lead to exhaustion skeletal regeneration potential replacement fibrotic fatty tissue. In this study we focused on unexplored strategy improve MuSC function niche based regenerative...
Duchenne muscular dystrophy (DMD) is a muscle disease caused by mutations in the dystrophin gene characterized myofiber fragility and progressive degeneration. The genetic defect results reduced number of self-renewing stem cells (MuSCs) an impairment their activation differentiation, which lead to exhaustion skeletal regeneration potential replacement fibrotic fatty tissue. In this study, we focused on unexplored strategy improve MuSC function preserve niche based regenerative properties...
Introduction: It is increasingly more evident that the bioactive factors produced by human Amniotic Mesenchymal Stromal Cells (hAMSCs) (i.e. secretome) can favor tissue repair and regeneration through modulation of immune system resolution inflammation. To date, biochemical nature these has yet to be defined. The integration several -omics technologies (e.g. proteomics, genomics, transcriptomics, lipidomics metabolomics) enable a multiapproach profiling hAMSC secretome. This study focuses on...
Human amniotic mesenchymal stromal cells (hAMSCs) have unique immunomodulatory properties making them attractive candidates for regenerative applications in inflammatory diseases. Most of their beneficial are mediated through secretome. The bioactive factors concurring to its therapeutic activity still unknown. Evidence suggests synergy between the two main components secretome, soluble and vesicular fractions, pivotal shifting inflammation promoting self-healing. Biological variability...