The possibility to isolate canine mesenchymal stem cells (MSCs) from foetal adnexa is interesting since several genetic disorders are reported resemble similar dysfunctions in humans. In this study, we successfully isolated, cytogenetically and molecularly characterized, followed the differentiation potency of MSCs adnexa, such as amniotic fluid (AF), membrane (AM), umbilical cord matrix (UCM). three types cell lines, morphology proliferating typically appeared fibroblast-like, population...

Extracellular vesicles (EVs) are important mediators of cell-cell communication thanks to their ability transfer bioactive cargo, thus regulating a variety physiological contexts. EVs derived from amniotic mesenchymal/stromal cells (eAMC-EVs) internalized by equine endometrial (eECs) with positive effects on regenerative medicine treatments. As the cellular uptake is influenced glycan profile both and target cells, this study focused role surface glycans in eAMC-EVs recipient eECs. Equine...

The aim of this work was to isolate, for the first time, progenitor-like cells from epithelial (AECs) and mesenchymal (AMCs) portions horse amniotic membrane, define biological properties these cells. AECs displayed polygonal morphology, while AMCs were fibroblast-like. Usually, six eight passages reached before proliferation decreased, with 13.08 26.5 cell population doublings attained after 31 days AMCs, respectively. Immunocytochemical studies performed at passage 3 (P3) showed that both...

We have recently demonstrated that heterologous transplantation of horse amniotic membrane-derived mesenchymal cells (AMCs) can be useful for cell therapy applications in tendon diseases, and hypothesized these may promote repair via paracrine-acting molecules targeting inflammatory processes. To test this hypothesis, here we examined the immunomodulatory characteristics AMCs their conditioned medium (AMC-CM) vitro, studied potential therapeutic effect AMC-CM thirteen different spontaneous...

Endometritis reduces fertility and is responsible for major economic losses in beef dairy industries. The aim of this study was to evaluate an alternative therapy using platelet-rich plasma (PRP). PRP tested vivo, after bovine intrauterine administration, vitro on endometrial cells.Bovine cells were cultured until passage (P) 10 with 5 % or PRP. Effect cell proliferation the expression genes [prostaglandin-endoperoxide synthase 2 (COX2), tumor protein p53 (TP53), oestrogen receptors (ER-α...

The effect of conditioned medium (CM) or microvesicles (MVs), secreted by multicellular spheroids oviductal cells, and the involvement some microRNAs (miRNAs) were investigated in canine oocyte maturation. To generate CM, cultured for 3 days. MVs obtained ultracentrifugation CM at 100,000 g measured size concentration NanoSight instrument. Cumulus-oocyte complexes (COCs) matured 38.5°C with 5% CO2 O2 synthetic fluid (SOF) biphasic systems: 24 h, 5.0 μg/mL LH other 48 h 10% oestrous bitch...

Amnion and amniotic fluid (AF) are noncontroversial inexhaustible sources of mesenchymal stem cells (MSCs) that can be harvested noninvasively at low cost. As in humans, also veterinary field, presumptive derived from these tissues reveal as promising candidates for disease treatment, specifically their plasticity, reduced immunogenicity, high anti-inflammatory potential. The aim this work is to obtain characterize, the first time bovine species, MSCs epithelial portion amnion (AECs) AF...

It is known that a paracrine mechanism exists between mesenchymal stem cells and target cells. This process may involve microvesicles (MVs) as an integral component of cell-to-cell communication.In this context, study aims to understand the efficacy MVs in in-vitro endometrial stressed view potential healing in-vivo studies. For purpose, presence type secreted by amniotic (AMCs) were investigated response was studied using dose-response curve at different concentrations times. Moreover,...

Administration of horse amniotic mesenchymal cells (AMCs) and their conditioned medium (AMC-CM) improves the in vivo recovery spontaneous equine tendon lesions inhibits vitro proliferation peripheral blood mononuclear (PBMC). This process may involve microvesicles (MVs) as an integral component cell-to-cell communication during tissue regeneration. In this study, presence type MVs secreted by AMCs were investigated response to was studied using a dose–response curve at different...

Umbilical cord matrix (UCM) has been recently proposed as an alternative source of mesenchymal stem cells (MSCs). The aim this study was to isolate and characterize presumptive from intervascular perivascular equine UCM obtain homogeneous subpopulations both sites.Umbilical cords were processed for retrieval MSCs. Unsieved portions evaluated cell cycle analysis immunophenotyping by flow cytometry. Cells each site separated into larger smaller sieved populations using multi-dishes with 8-μm...

Abstract Introduction While amniotic mesenchymal cells have been isolated and characterized in different species, epithelial (AECs) found only humans horses are recently considered valid candidates regenerative medicine. The aim of this work is to obtain characterize, for the first time feline presumptive stem from portion amnion be used clinical applications. Methods In our study, we molecularly induced vitro differentiation AECs, obtained after enzymatic digestion amnion. Results AECs...

This is the first study comparing stemness features of equine mesenchymal progenitor cells derived from amniotic membrane and bone marrow.To investigate an alternative noninvasive stromal cell source for tissue engineering.In vitro experimental characteristics amnion marrow.Cells isolated marrow were analysed proliferation (growth curve, doubling time, colony forming unit). Immunocytochemical detection pluripotency markers gene expression also performed these studied multilineage...

Amniotic membrane-derived mesenchymal cells (AMCs) are considered suitable candidates for a variety of cell-based applications. In view cell therapy application in uterine pathologies, we studied AMCs comparison to isolated from the endometrium mares at diestrus (EDCs) being during and early pregnancy similar hormonal standpoint. particular, demonstrated that amnion tissue fragments (AM) shares same transcriptional profile with endometrial (ED), expressing genes involved (AbdB-like Hoxa...

A repeat breeder cow (RBC) can be defined as an animal that after 3 or more inseminations cannot get pregnant because of fertilization failure early embryonic death. If no cause is identified precisely, inadequate uterine receptivity responsible for implantation failures. Since a large number molecular mediators, such cytokines, growth factors and lipids have been postulated to involved in feto-maternal interaction, this study different approach the treatment RBC syndrome has employed using...

Abstract Background The identification of the adipocyte-derived obesity gene product, leptin (Ob), and subsequently its association with reproduction in rodents humans led to speculations that may be involved regulation oocyte preimplantation embryo development. In mice pigs, vitro addition significantly increased meiotic resumption promoted development a dose-dependent manner. This study was conducted determine whether supplementation during maturation (IVM) horse oocytes could have effects...

Cell-derived microvesicles (MVs) are a recently discovered mechanism of cell-to-cell communication. Our previous data show that MVs secreted by equine amniotic mesenchymal-derived cells (AMCs) involved in downregulation proinflammatory genes lipopolysaccharide-stressed tendon and endometrial cells. The aim the present study was to evaluate whether AMC-MVs contain selected microRNAs (miRNAs) inflammation. Two pools cells, derived from 3 membranes each, their respective were collected. Small...

Recent studies on cull cows have shown that ovarian abnormalities, particularly insufficiency, are a common cause of reproductive failure. The aim this study was to treat bovine failure with intraovarian administration autologous platelet rich plasma (PRP), which is in growth factors, chemokines and cytokines, could stimulate follicular steroidogenesis. Twelve hypofunction were enrolled the theywere randomly allocated control group (CTR) treated (six animal for group). In group, only five...