A5075909390- Milk Quality and Mastitis in Dairy Cows
- Probiotics and Fermented Foods
- Microbial infections and disease research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Antimicrobial Resistance in Staphylococcus
- Mycobacterium research and diagnosis
- Animal Disease Management and Epidemiology
- Parasitic Infections and Diagnostics
- Veterinary medicine and infectious diseases
- Vector-Borne Animal Diseases
- Helicobacter pylori-related gastroenterology studies
- Microbial Metabolism and Applications
- Nail Diseases and Treatments
- Salmonella and Campylobacter epidemiology
- Infectious Diseases and Mycology
- Coccidia and coccidiosis research
- Virology and Viral Diseases
- Bacteriophages and microbial interactions
- Plant and fungal interactions
- Virus-based gene therapy research
- Enterobacteriaceae and Cronobacter Research
- Animal Behavior and Welfare Studies
- T-cell and Retrovirus Studies
- Bacterial biofilms and quorum sensing
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"
2015-2024
National Research Council
2020-2024
Deleted Institution
2020-2024
Istituto di Biologia e Biotecnologia Agraria
2020
University of Milan
2011
Ospedale Maggiore di Lodi
2009-2011
Recordati (Italy)
2011
Cornell University
2011
Abstract Prototheca zopfii ( P. , class Trebouxiophyceae, order Chlorellales, family Chlorellaceae), a non-photosynthetic predominantly free-living unicellular alga, is one of the few pathogens belonging to plant kingdom. This alga can affect many vertebrate hosts, sustaining systemic infections and diseases such as mastitis in cows. The aim our work was sequence assemble genotype 1 2 mitochondrial plastid genomes. Remarkably, (38 Kb) (28 genomes are models compaction smallest known...
Influenza D virus (IDV), a new member of the Orthomyxoviridae family, was first reported in 2011 swine Oklahoma, and consequently found cattle across North America Eurasia. To investigate circulation IDV among pigs Italy, period between June 2015 May 2016, biomolecular virological tests were performed on 845 clinical samples collected from 448 pig farms affected by respiratory distress located Po Valley. Serological conducted 3698 sera, including archive sera 2009, as well same region. Viral...
SUMMARY Staphylococcus aureus is the most important causative agent of subclinical mastitis in cattle resulting reduced milk production and quality. Methicillin-resistant S. (MRSA) strains has a clear zoonotic relevance, especially case occupational exposure. The aim study was to evaluate prevalence MRSA bulk tank (BTM) from dairy herds Lombardy Region (Northern Italy) identify main circulating genotypes. were characterized by susceptibility testing, multi-locus sequence typing (MLST), spa...
Staphylococcus aureus (Staph. aureus) is one of the major pathogens causing mastitis in dairy ruminants worldwide. The chronic nature Staph. infection enhances contagiousness risk and diffusion herds. In order to identify factors involved intra-mammary (IMI) cows, we investigated molecular characteristics two groups strains belonging ST8 ST398, differing clinical properties, through comparison whole genome transcriptome sequencing.The strains, originated from high IMI prevalence herds other...
ABSTRACT Thermotolerant Campylobacter spp. are frequently the cause of human gastroenteritis and have assumed more importance in Italy following increased consumption raw milk. Our objectives were to determine prevalence genotypes dairy herds investigate possible sources bulk milk contamination. Bulk from ( n = 282) was cultured for Enterobacteriaceae . At three jejuni -positive farms, bovine feces, pigeon intestines, milk, water points also investigated. Isolates identified by PCR genotyped...
Dairy products can harbor various microorganisms (e.g., Campylobacter spp., Salmonella Listeria monocytogenes, verocytotoxin-producing Escherichia coli) arising from animal reservoirs, and which become important sources of foodborne illness. Therefore, early detection food pathogens is crucial to prevent diseases. We wished develop an accurate quantitative protocol based on a droplet digital polymerase chain reaction (ddPCR) involving eight individual TaqMan™ reactions detect simultaneously,...
Background Mycobacterium avium subsp. paratuberculosis (MAP) causes chronic enteritis in a wide range of animal species. In cattle, MAP disease called Johne's disease, or paratuberculosis, that is not treatable and the efficacy vaccine control controversial. The clinical phase characterised by diarrhoea, weight loss, drop milk production eventually death. Susceptibility to infection heritable with heritability estimates ranging from 0.06 0.10. There have been several studies over last few...
Staphylococcus aureus (S. aureus) is a major agent of dairy cow intramammary infections: the different prevalences mastitis reported might be related to combination S. virulence factors beyond host factors. The present study considered 169 isolates from Italian herds that were classified into four groups based on prevalence infection at first testing: low (LP), medium-low (MLP), medium-high (MHP) and high (HP). We aimed correlate presence genes with infections in order develop new strategies...
Abstract Background: In dogs, the gastric Helicobacter spp. have been well studied, but there is little information regarding other parts of alimentary system. We sought to determine spatial distribution in gastrointestinal tract and hepatobiliary system dogs using culture‐independent methods. Materials methods: Samples stomach, duodenum, ileum, cecum, colon, pancreas, liver, bile from six were evaluated for by genus, gastric, enterohepatic Polymerase chain reaction, 16S rRNA gene sequence...
Staphylococcus aureus is a major agent of bovine mastitis in dairy herds, causing economic losses industry worldwide. In addition, milk and milk-products contaminated by Staph. can cause harmful human diseases. The aim this study was to characterize strains isolated from farms Tunisia. Bulk tank (n = 32) individual cow 130) samples were collected during the period 2013-2014. Forty-three isolates recovered typed spa typing, 16S-23S rRNA intergenic spacer (RS-PCR) multiplex PCRs for 22...
Abstract Thirty-six representative velogenic strains of Newcastle disease virus isolated in Italy since 1960 were characterized by restriction site and partial sequence analyses the fusion protein gene. Viruses belonging to six known genotypes Lomniczi et al . found. Genotype IV, which was most probably main epizootic group Europe before war, responsible for outbreaks 1960s persisted until late 1980s Italy. An peak 1972 1974 coincided with appearance genotype V viruses that present more than...
Monitoring animal welfare (AW) in pig farms requires both proper indicators and a feasible approach. Animal-based measures (ABMs) are well-established AW indicators. Furthermore, screening at the slaughterhouses could be useful for identifying problems on farm. The aim of this study was to evaluate ABMs slaughterhouse and, when possible, compare these with those collected carried out northern Italy commercial abattoir sample farms. were recorded pigs from 62 batches 54 farms, during...
Background Ventilation with the noble gas argon (Ar) has shown neuroprotective and cardioprotective properties in different vitro vivo models. Hence, effects of Ar were investigated a severe, preclinically relevant porcine model cardiac arrest. Methods Results Cardiac arrest was ischemically induced 36 pigs left untreated for 12 minutes before starting cardiopulmonary resuscitation. Animals randomized to 4-hour post-resuscitation ventilation with: 70% nitrogen-30% oxygen (control); 50%...
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Bovine mastitis caused by Prototheca spp. infection is increasing worldwide, therefore becoming more relevant to the dairy industry. Almost all isolates from bovine mammary protothecosis came P. zopfii genotype 2, with a lower prevalence of due blaschkeae and rarely wickerhamii. In this study, we report development two multiplex PCR assays able discriminate among three species responsible for intramammary (IMI). Our assay based on specific amplification new DNA target mitochondria...