A5026160772- Mycobacterium research and diagnosis
- Liver Disease Diagnosis and Treatment
- Milk Quality and Mastitis in Dairy Cows
- Ginseng Biological Effects and Applications
- Microbial infections and disease research
- Probiotics and Fermented Foods
- Drug Transport and Resistance Mechanisms
- Bacteriophages and microbial interactions
- Listeria monocytogenes in Food Safety
- Infectious Diseases and Mycology
- Cholesterol and Lipid Metabolism
- Diet and metabolism studies
- Salmonella and Campylobacter epidemiology
- Identification and Quantification in Food
- Genomics and Phylogenetic Studies
- Microbial Inactivation Methods
- Neonatal and Maternal Infections
- Gut microbiota and health
- Powdery Mildew Fungal Diseases
- Pharmacological Effects of Natural Compounds
- Biopolymer Synthesis and Applications
- Hepatitis C virus research
- Lipid metabolism and disorders
- Bacterial Identification and Susceptibility Testing
- Veterinary medicine and infectious diseases
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"
2016-2025
Ospedaliera di Piacenza
2014
University of Modena and Reggio Emilia
2005-2012
Azienda Unita' Sanitaria Locale Di Modena
2007-2009
Stichting HIV Monitoring
2009
Abstract Background and Aim: Studies have shown monounsaturated oleic acid to be less toxic than palmitic prevent/attenuate hepatocites toxicity in steatosis models vitro . However, what degree these effects are mediated by extent is unknown. Methods: We evaluated whether per se associated with hepatocytes apoptosis determined the role of acid, most abundant fatty acids western diets, on triglyceride accumulation an model induced three hepatocytic cell lines (HepG2, HuH7, WRL68). The impact...
Abstract Prototheca zopfii ( P. , class Trebouxiophyceae, order Chlorellales, family Chlorellaceae), a non-photosynthetic predominantly free-living unicellular alga, is one of the few pathogens belonging to plant kingdom. This alga can affect many vertebrate hosts, sustaining systemic infections and diseases such as mastitis in cows. The aim our work was sequence assemble genotype 1 2 mitochondrial plastid genomes. Remarkably, (38 Kb) (28 genomes are models compaction smallest known...
Salmonella is diffused worldwide, and enterica subsp. spread worldwide with many serovars associated the infection of domestic bovines. The most are S. Dublin, Typhimurium Infantis. Napoli is, however very rarely reported in ruminants. Here, we report an outbreak on a dairy cow farm Northern Italy (Piacenza). A total 18 isolates were recovered from aborted fetuses, feces, tissues environmental samples. Whole genome sequencing suggested that all belonged to same cluster. After application...
The demand for rapid methods the quantification of pathogens is increasing. Among these methods, those based on nucleic acids amplification (quantitative PCRs) are most widespread worldwide. Together with qPCR, a new approach named digital PCR (dPCR), has rapidly gained importance. aim our study was to compare results obtained using two different dPCR systems and one qPCR in three bacterial pathogens: Listeria monocytogenes, Francisella tularensis, Mycobacterium avium subsp....
Animals' exchanges are considered the most effective route of between-farm infectious disease transmission. However, despite being often overlooked, infection spread due to contaminated equipment, vehicles, or personnel proved be important for several livestock epidemics. This study investigated role indirect contacts in a potential dairy farm network Province Parma (Northern Italy). We built contact networks using data on cattle exchange (direct contacts), and on-farm visits by...
Abstract Background Recent data highlighted the role of nuclear receptors in transcriptional regulation limiting enzyme bile acid synthesis, cholesterol 7α‐hydroxylase, cellular and animal models. This study was designed to analyze effects age on 7α‐hydroxylase related receptor expression human livers. Design Surgical liver biopsies were obtained 23 patients requiring operation gastrointestinal tract. mRNA levels co‐activators assayed by quantitative real‐time RT‐PCR. Serum...
Real-time reverse transcription PCR is currently the most sensitive method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Defining whether a patient could be contagious or not in presence of residual SARS-CoV-2 RNA extreme importance context public health. In this prospective multicenter study, virus isolation was prospectively attempted 387 nasal swabs from clinically recovered patients showing low viral load (quantification cycle, Cq, value greater than 30). The...
Paratuberculosis is an infectious disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). MAP intracellular pathogen with a possible zoonotic potential since it has been successfully isolated from the intestine and blood Crohn's patients.Since no cure available, after detection disease, animal culling sole applicable containment strategy. However, difficult in its subclinical form, facilitates spread raising need for development effective diagnosis vaccination...
Consumption of milk and dairy products is considered one the main routes human exposure to Mycobacterium avium subsp. paratuberculosis (MAP). Quantitative data on MAP load in raw cows' are essential starting point for assessment. Our study provides this information a regional scale, estimating bulk tank (BTM) produced Emilia-Romagna region (Italy). The survey was carried out 2934 BTM samples (88.6% farms herein present) using two different target sequences qPCR (f57 IS900). Data about...
BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) and chronic hepatitis C feature steatosis insulin resistance (IR), conditions associated with the metabolic syndrome (MS). OBJECTIVES: To assess prevalence of MS determinants IR in patients NAFLD C. METHODS: Ninety-three consecutive NAFLD, 97 virus (HCV) genotypes 1 2, 182 ‘healthy’ controls without were enrolled present study. The was assessed by modified Adult Treatment Panel III criteria homeostasis model assessment (HOMA-IR). defined...
Mycobacterium avium subsp. paratuberculosis (MAP) is the cause of a chronic enteritis ruminants (bovine (PTB)--Johne's disease) that associated with enormous worldwide economic losses for animal production. Diagnosis based on observation clinical signs, detection antibodies in milk or serum, evaluation bacterial culture from feces. The limit these methods they are not able to detect disease subclinical stage and applicable only when already advanced. For this reason, main purpose study use...
The causative agent of paratuberculosis in ruminants, Mycobacterium avium subsp. (MAP), although still a matter debate, has been linked with Crohn's and other human diseases. availability rapid methods for assessing the viability MAP cells food, particular milk, could be great use risk management food safety. is generally assessed using culture techniques that require prolonged incubation periods growth MAP. To differentiate between viable nonviable milk samples, this study explores...
Paratuberculosis is a chronic enteric infection, caused by Mycobacterium avium subsp. paratuberculosis (MAP), affecting virtually all ruminants as well other animals. MAP also suspected to be involved in the etiology of some human diseases, like Crohn's disease and others. In surveillance studies, different analytical methodologies were employed detect MAP, showing results incidence dairy products. The aim this study was evaluate performance characteristics three methods [culture,...
Abstract Background Cholesterol cholelithiasis (gallstone disease) is a common disease in the Western world. The aim of present study was to analyze hepatic expression number nuclear receptors involved bile acid metabolism human cholesterol gallstone disease. Materials and methods Surgical liver biopsies were obtained from 11 patients with untreated nine gallstone‐free subjects; mRNA levels 7α‐hydroxylase (CYP7A1) related coactivators assayed by quantitative real‐time RT‐PCR. Results No...