A5038437738- Peptidase Inhibition and Analysis
- Cell Adhesion Molecules Research
- Protease and Inhibitor Mechanisms
- HER2/EGFR in Cancer Research
- Monoclonal and Polyclonal Antibodies Research
- Calpain Protease Function and Regulation
- Osteoarthritis Treatment and Mechanisms
- Bacterial Identification and Susceptibility Testing
- Toxoplasma gondii Research Studies
- Bone Tumor Diagnosis and Treatments
- Sarcoma Diagnosis and Treatment
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Extracellular vesicles in disease
- Biosensors and Analytical Detection
- Blood Coagulation and Thrombosis Mechanisms
- Antifungal resistance and susceptibility
- Cancer-related gene regulation
Ri.MED
2020-2024
Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione
2020-2024
University of Palermo
2022-2024
Abstract Matrix metalloproteinases (MMPs) and the related families of disintegrin (ADAMs) ADAMs with thrombospondin repeats (ADAMTSs) play a crucial role in extracellular matrix (ECM) turnover shedding cell-surface molecules. The proteolytic activity is post-translationally regulated by their endogenous inhibitors, known as tissue inhibitors (TIMPs). Several MMPs, ADAMTSs TIMPs have been reported to be endocytosed low-density lipoprotein receptor-related protein-1 (LRP-1). Different binding...
Abstract Proteolytic release of transmembrane proteins from the cell surface, so called ectodomain shedding, is a key process in inflammation. Inactive rhomboid 2 (iRhom2) plays crucial role this context, that it guides maturation and function sheddase ADAM17 (a disintegrin metalloproteinase 17) immune cells, and, ultimately, its ability to inflammatory mediators such as tumor necrosis factor α (TNFα). Yet, macrophage sheddome iRhom2/ADAM17, which collection substrates are released by...
The low-density lipoprotein receptor-related protein 1 (LRP1) is a cell-surface receptor ubiquitously expressed in various tissues. It plays tissue-specific roles by mediating endocytosis of diverse range extracellular molecules. Dysregulation LRP1 involved multiple conditions including osteoarthritis (OA) but little information available about the specific profile direct binding partners (ligandome) for each tissue, which would lead to better understanding its role disease states. Here, we...
The adamalysin metalloproteinase 15 (ADAM15) has been shown to protect against development of osteoarthritis in mice. Here, we have investigated factors that control ADAM15 levels cartilage. Secretomes from wild-type and Adam15−/− chondrocytes were compared by label-free quantitative mass spectrometry. mRNA was isolated murine knee joints, either with or without surgical induction on male C57BL/6 mice, the expression Adam15 other related genes quantified RT-qPCR. human normal osteoarthritic...
ADAM15 is a member of the disintegrin-metalloproteinase family sheddases, which plays role in several biological processes including cartilage homeostasis. In contrast with well-characterized ADAMs, such as canonical sheddases ADAM17 and ADAM10, little known about substrates or how enzyme exerts its functions. Herein, we used "surface-spanning enrichment click-sugars (SUSPECS)" proteomics to identify and/or proteins regulated by proteinase at cell surface chondrocyte-like cells. Silencing...
Chondrosarcoma is the second most common bone tumor, accounting for 20% of all cases. Little known about pathology and molecular mechanisms involved in development metastatic process chondrosarcoma. As a consequence, there are no approved therapies this tumor surgical resection only treatment currently available. Moreover, available biomarkers type chondrosarcoma classification relies on operator-dependent histopathological assessment. Reliable urgently needed, as well greater understanding...
Ectodomain shedding is a key mechanism of several biological processes, including cell-communication. Disintegrin and metalloproteinases (ADAMs), together with the membrane-type matrix metalloproteinases, play pivotal role in transmembrane proteins. Aberrant associated to pathological conditions, arthritis. Tissue inhibitor metalloproteases 3 (TIMP-3), an endogenous ADAMs (MMPs), has been proven be beneficial such diseases. Thus, strategies increase TIMP-3 bioavailability tissue have sought...
A disintegrin and metalloproteinase 15 (ADAM15) is a member of the ADAM family sheddases. Its genetic ablation in mice suggests that ADAM15 plays an important role wide variety biological functions, including cartilage homeostasis. Nevertheless, while substrate repertoire other members family, ADAM10 ADAM17, largely established, little known about substrates how it exerts its functions. Herein, we used unbiased proteomics to identify proteins regulated by proteinase chondrocyte-like HTB94...
Ectodomain shedding, which is the proteolytic release of transmembrane proteins from cell surface, crucial for cell-to-cell communication and other biological processes. The metalloproteinase ADAM17 mediates ectodomain shedding over 50 ranging cytokines growth factors, such as TNF EGFR ligands, to signaling receptors adhesion molecules. Yet, sheddome only partly defined functions protease have not been fully characterized. Some substrates (e.g. HB-EGF) are known bind heparan sulfate...
ABSTRACT Ectodomain shedding, which is the proteolytic release of transmembrane proteins from cell surface, crucial for cell‐to‐cell communication and other biological processes. The metalloproteinase ADAM17 mediates ectodomain shedding over 50 ranging cytokines growth factors, such as TNF EGFR ligands, to signalling receptors adhesion molecules. Yet, sheddome only partly defined functions protease have not been fully characterized. Some substrates (e.g., HB‐EGF) are known bind heparan...
Abstract The low-density lipoprotein receptor-related protein 1 (LRP1) is a cell-surface receptor ubiquitously expressed in adult tissues. It plays tissue-specific physiological roles by mediating endocytosis of diverse range extracellular molecules. Dysregulation LRP1 involved multiple conditions including Alzheimer’s disease, atherosclerosis and osteoarthritis (OA). However, little information available about the specific ligand profile (ligandome) for each tissue, which would lead to...
Theoretically, Aspergillus spp. grow in culture media, but frequently, blood cultures of patients with invasive Aspergillosis are negative, even if until now, the reasons not clear. This aspect underlines lack a good strategy for cultivation and isolation In order to develop complete analytical method detect clinical pharmaceutical samples, we investigated growth performance two systems versus pharmacopeia standard method. At <72 h, all test showed comparable sensitivity, about 1−2 conidia....