A5059416186- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- Glycosylation and Glycoproteins Research
- Cell death mechanisms and regulation
- Congenital heart defects research
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- Connective Tissue Growth Factor Research
- Signaling Pathways in Disease
- Genetic factors in colorectal cancer
- Tissue Engineering and Regenerative Medicine
- Mosquito-borne diseases and control
- Cancer Cells and Metastasis
- Autism Spectrum Disorder Research
- Systemic Sclerosis and Related Diseases
- Cell Adhesion Molecules Research
- Genetics and Neurodevelopmental Disorders
- RNA Research and Splicing
- Viral Infectious Diseases and Gene Expression in Insects
- Caveolin-1 and cellular processes
- Ubiquitin and proteasome pathways
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Electrospun Nanofibers in Biomedical Applications
- Salivary Gland Disorders and Functions
Istituto Superiore di Sanità
2013-2021
The University of Texas MD Anderson Cancer Center
2013
Despite the use of multimodality therapy using cisplatin to treat patients with advanced stage squamous cell carcinoma head and neck (HNSCC), there is an unacceptably high rate treatment failure. TP53 most commonly mutated gene in HNSCC, impact p53 mutation on response poorly understood. Here, we show unambiguously that wild-type (wtp53) associated sensitivity HNSCC cells treatment, whereas or loss resistance. We also senescence major cellular wtp53 resistance p53-null -mutant due their lack...
Zika virus (ZIKV) is a flavivirus with marked effect on fetal nervous system development. ZIKV treatment has recently been found to also have benefit against glioblastoma, highly aggressive brain tumor poor prognosis. The reported data do not completely explain the mechanism beyond this effect. Nevertheless, in majority of cases no adverse healthy adult humans. In study, we characterized infection glioblastoma stem cells, which are considered responsible for progression and resistance...
MiR34 involvement in myocardial injury repair and ageing has been well documented mouse model. Our aim was to establish whether the inhibition of miR34 expression through locked nucleic acid (LNA) could be used as a pharmacological intervention enhance human heart repair. Cardiac progenitor cells were obtained by right atrial specimen collection during intraoperative procedures. analysis revealed direct correlation between patient age, its silencing LNA promoted cardiac growth rate up...
Coimmunoprecipitation (co-IP) is one of the most frequently used techniques to study protein-protein (PPIs) or protein-nucleic acid interactions (PNIs). However, presence coprecipitated contaminants a well-recognized issue associated with single-step co-IPs. To overcome this limitation, we developed two-step co-IP (TIP) strategy that enables sequential coimmunoprecipitations endogenous protein complexes. TIP can be performed broad range mono- and polyclonal antibodies targeting single...
The epidermis is a stratified epithelium with stem cell subpopulation in the basal layer that constantly replicates and periodically detaches from base, undergoing differentiation process involves various developmental signals regulatory pathways. During last 10 years, number of studies tried to elucidate intricate scenario maintains epithelial shield during entire life span. In our study, we investigated role Numb skin compartment and, particular, its involvement maintenance. expression was...
Abstract In developed countries, cardiovascular diseases are currently the first cause of death. Cardiospheres (CSs) and cardiosphere‐derived cells (CDCs) have been found to ability regenerate myocardium after myocardial infarction (MI). recent years, much effort has made gain insight into human heart repair mechanisms, in which miRNAs shown play an important role. this regard, elucidate involvement miRNAs, we evaluated miRNA expression profile across biopsy, CSs CDCs using microarray...
Abstract In the past few decades, numerous approaches have been developed to investigate protein‐protein and protein‐nucleic acid interactions (PPIs PNIs). Affinity purification methods such as co‐immunoprecipitation (co‐IP) are commonly used detect isolate macromolecular complexesresulting from these interactions. this article, we describe a two‐step (TIP) technique. As compared standard co‐IP, TIP provides increased specificity in isolation of PPIs or PNIs under native expression...
Abstract Despite the use of multimodality therapy employing cisplatin to treat patients with advanced stage head and neck squamous cell carcinoma (HNSCC), there is an unacceptably high rate treatment failure. TP53 most commonly mutated gene in HNSCC, impact p53 mutation on response poorly understood. Here we show unambiguously that wild type (wtp53) associated sensitivity HNSCC cells while or loss resistance. We also demonstrate senescence major mode death accounts for responses wtp53...
<div>Abstract<p>Despite the use of multimodality therapy using cisplatin to treat patients with advanced stage squamous cell carcinoma head and neck (HNSCC), there is an unacceptably high rate treatment failure. <i>TP53</i> most commonly mutated gene in HNSCC, impact p53 mutation on response poorly understood. Here, we show unambiguously that wild-type TP53 (wtp53) associated sensitivity HNSCC cells treatment, whereas or loss resistance. We also senescence major...
<p>PDF file - 55 KB</p>
<p>PDF file - 5 MB, Apoptosis is not the main mode of cell death in wtp53 HNSCC cells.</p>
<p>PDF file - 55 KB</p>
<p>PDF file - 5 MB, Apoptosis is not the main mode of cell death in wtp53 HNSCC cells.</p>
<div>Abstract<p>Despite the use of multimodality therapy using cisplatin to treat patients with advanced stage squamous cell carcinoma head and neck (HNSCC), there is an unacceptably high rate treatment failure. <i>TP53</i> most commonly mutated gene in HNSCC, impact p53 mutation on response poorly understood. Here, we show unambiguously that wild-type TP53 (wtp53) associated sensitivity HNSCC cells treatment, whereas or loss resistance. We also senescence major...