A5044689322- Mitochondrial Function and Pathology
- Phytochemicals and Antioxidant Activities
- Photosynthetic Processes and Mechanisms
- Antioxidant Activity and Oxidative Stress
- Seed and Plant Biochemistry
- ATP Synthase and ATPases Research
- Free Radicals and Antioxidants
- Heavy Metals in Plants
- Plant Stress Responses and Tolerance
- Consumer Attitudes and Food Labeling
- Food composition and properties
- Adipose Tissue and Metabolism
- Wheat and Barley Genetics and Pathology
- Genomics, phytochemicals, and oxidative stress
- Crop Yield and Soil Fertility
- Biochemical and biochemical processes
- GABA and Rice Research
- Advanced Glycation End Products research
- Plant Micronutrient Interactions and Effects
- Lipid metabolism and biosynthesis
- Plant responses to water stress
- Genetics and Plant Breeding
- Venomous Animal Envenomation and Studies
- Autophagy in Disease and Therapy
- Weed Control and Herbicide Applications
University of Foggia
2013-2023
Ambiente Italia (Italy)
2016
Istituto Sperimentale per la Zootecnia
2001-2004
University of Molise
2001-2004
The carrot is one of the most cultivated vegetables in world. Black or purple carrots contain acylated anthocyanins which are special interest to food industry for their stability and nutraceutical characteristics. Anthocyanin-rich fruits have gained popularity last ten years, due health benefits they provide. In this paper, characterizations bioactive compounds antioxidant capacities different anthocyanin-containing (a black carrot—BC, a local carrot, “Polignano” carrot—PC), compared...
In order to gain a first insight into the alternative oxidase (AO) function in durum wheat mitochondria (DWM), we investigated some activation pathways of this enzyme DWM purified from both etiolated shoots and green leaves. AO was activated when were added with either pyruvate, known as an activator other plant mitochondria, or alanine plus 2-oxoglutarate, which can generate intramitochondrial pyruvate glutamate via transamination. contrast, no activity observed during oxidation malate...
Abstract: Antioxidant activity (AA) of quinoa ( Chenopodium Willd.) seeds, as well durum wheat Triticum turgidum L. ssp. Desf.) and emmer T . dicoccum Schübler) grains, was evaluated by studying hydrophilic (H), lipophilic (L), free‐soluble (FSP) insoluble‐bound (IBP) phenolic extracts using the new lipoxygenase/4‐nitroso‐ N,N‐ dimethylaniline (LOX/RNO) method, able to simultaneously detect different antioxidant mechanisms, Oxygen Radical Absorbance Capacity (ORAC) Trolox Equivalent (TEAC)...
The effect of free fatty acids (FFAs) and acyl-CoA esters on K+ uptake was studied in mitochondria isolated from durum wheat (Triticum Desf.), a species that has adapted well to the semi-arid Mediterranean area possessing highly active mitochondrial ATP-sensitive channel (PmitoKATP), may confer resistance environmental stresses. This made by swelling experiments KCl solution under experimental conditions which PmitoKATP activity monitored. Linoleate other FFAs (laurate, palmitate, stearate,...
ABSTRACT The plant uncoupling mitochondrial protein (PUMP) and the potassium channel (PmitoK ATP ) are two recently discovered energy‐dissipating systems present in mitochondria, which may play a role as defence under environmental stress. To verify whether hyperosmotic stress affects dissipating durum wheat ( Triticum Desf.), their functioning was studied early etiolated seedlings maintained moderate severe salt (NaCl) osmotic (mannitol) As measures of integrity, membrane potential...
To date, the existence of plant inner membrane anion channel (PIMAC) has been shown only in potato mitochondria, but its physiological role remains unclear. In this study, by means swelling experiments K+ and ammonium salts, we characterize a PIMAC-like anion-conducting pathway mitochondria from durum wheat (DWM), monocotyledonous species phylogenetically far potato. DWM were investigated since they possess very active potassium (PmitoKATP), so implying matching uniport and, possibly,...
The health-promoting properties of natural plant bioactive compounds are mainly attributable to their ability counteract oxidative stress. This is considered a major causative factor in aging and aging-related human diseases, which causal role also ascribed dicarbonyl due accumulation methylglyoxal (MG) other reactive species, leading macromolecule glycation cell/tissue dysfunction. glyoxalase (GLYI) enzyme, catalyzing the rate-limiting step GSH-dependent MG detoxification pathway, plays key...
The important role of plant mitochondria in the adaptation to environmental stresses at subcellular level has recently emerged. In particular, an mitochondrial mechanism involved resistance is inhibition proline oxidation. order study this physiological mechanism, we used both washed and purified durum wheat ( Triticum ) (DWM) isolated from early seedlings germinated two different NaCl solutions leading either moderate or severe damage growth. To assess contribution osmotic component stress,...
In order to gain a first insight into the effects of reactive oxygen species (ROS) on plant mitochondria, we studied effect ROS producing system consisting xanthine plus oxidase rate membrane potential (Δ Ψ ) generation due either succinate or NADH addition durum wheat mitochondria as monitored by safranin fluorescence. We show that early production inhibits succinate‐dependent, but not NADH‐dependent, Δ and uptake. This inhibition appears depend impairment mitochondrial permeability...
In this study the 4-nitroso-N,N-dimethylaniline (RNO) bleaching associated with linoleic acid hydroperoxidation catalyzed by soybean lipoxygenase (LOX)-1 isoenzyme (LOX/RNO reaction) was used to determine antioxidant activity (AA) of hydrophilic and lipophilic pure compounds mixtures antioxidants extracted from durum wheat whole flour (DWWF). By means a simple rapid experimental protocol (about 3 min/assay), LOX/RNO reaction may simultaneously detect many functions (scavenging some...
Durum wheat mitochondria (DWM) possess an ATP-inhibited K(+) channel, the plant mitoK(ATP) (PmitoK(ATP) ), which is activated under environmental stress to control mitochondrial ROS production. To do this, PmitoK(ATP) collapses membrane potential (ΔΨ), thus suggesting uncoupling. We tested this point by studying oxidative phosphorylation (OXPHOS) in DWM purified from seedlings and subjected both severe mannitol NaCl stress. In severely-stressed DWM, ATP synthesis via OXPHOS, continuously...
The new LOX-FL method highlights improvement of serum antioxidant capacity after intake better than widely used methods.