Two strategies are described for the construction of extended arrays transition metal-containing molecules linked via hydrogen bonds. The first approach connects metal to ligand bearing hydrogen-bonding group(s) a coordinative bond. Thus, coordination complexes with peripheral functional groups constitute (molecular) building blocks assembly. ligands, substituted pyridines, have been selected provide rigidity and thus permit geometry guide direction propagation hydrogen-bonded links between...

The synthesis of oligodeoxynucleotides (ODNs) containing the sensitive N6-acetyladenosine (6acA), N2-acetylguanosine (2acG), and N4-methyoxycarbonyldeoxycytidine (4mcC), as well up to four N4-acetyldeoxycytidine (4acC) modifications is described. meDmoc group was used for protection exo-amino groups nucleobases, Dmoc linker solid phase synthesis. Deprotection cleavage were achieved under non-nucleophilic conditions, which highly 4acC, 6acA, 2acG, 4mcC found completely stable. Among modified...

The unsaturated permeability coefficients are often used to solve geotechnical problems associated with soils. But it is very difficult measure. However, the can be predicted by Soil-water Characteristic Curves (SWCCs). Van Genuchten Model (VG model) rife as it’s smooth and good fitting, thus, has most research data. Therefore, data on VG model parameters ( α , n θ s r ) of Malan loess in Chinese Loess Plateau collected past two decades obtain spatial distribution characteristics parameters....

Synthetic oligodeoxynucleotides are purified with use of a catching by polymerization, washing, and releasing approach. The method does not require any chromatography, purification is achieved simple operations such as shaking, extraction. It therefore useful for large-scale synthetic oligonucleotide drugs. In addition to oligonucleotides, this polymerization concept expected be equally other oligomers peptides oligosaccharides.

Abstract SARS‐CoV‐2 causes individualized symptoms. Many reasons have been given. We propose that an individual's epitranscriptomic system could be responsible as well. The viral RNA genome can subject to modifications, which different for individuals, and thus epitranscriptomics affect many events including replication differently. In this context, we studied the effects of modifications pseudouridine (Ψ), 5‐methylcytosine (m 5 C), N 6‐methyladenosine 6 A), 1‐methyladenosine 1 A)...

The catching-by-polymerization (CBP) oligodeoxynucleotide (oligo or ODN) purification method has been demonstrated suitable for large-scale, parallel, and long oligo purification. authenticity of the oligos verified via DNA sequencing. Gene construction expression have demonstrated. A remaining obstacle to practical utility CBP is affordable polymerizable tagging phosphoramidites (PTPs) that are needed method. In this article, we report scalable synthesis four nucleoside (dA, dC, dG dT)...

The longest oligos that can be chemically synthesized using known methods are typically considered to 200-mers. Here, we report direct synthesis of an 800-mer green fluorescent protein (GFP) gene and a 1,728-mer Φ29 DNA polymerase on automated synthesizer. Key innovations enabled the breakthrough include conducting smooth surface glass wool or bead rather than within pores traditional solid supports, use powerful catching-by-polymerization (CBP) method for isolation full-length from crude...

A fluoride‐cleavable phosphoramidite for biotinylation was designed, synthesized and coupled efficiently to the 5′‐end of DNA on an automatic synthesizer. The diisopropylsilyl acetal functionality used link biotin moiety through a tertiary hydroxide group DNA. This linkage proved be completely stable under certain post‐synthetic cleavage/deprotection conditions [0.05 M K 2 CO 3 in MeOH, room temperature, 24 h MeNH (∼40%)/NH 4 OH (∼29%), 1:1 v/v, 65°C, 30 min] while it can readily broken by...

Lagrangian actinometry represents a new method of photochemical reactor characterization. The is based on an application dyed microspheres, which were developed by attachment (E)-5-[2-(methoxycarbonyl)ethenyl]cytidine (hereafter referred to as S) polystyrene microspheres. S nonfluorescent molecule that when subjected ultraviolet (UV) irradiation yields single product, 3-β-D-ribofuranosyl-2,7-dioxopyrido[2,3-d]pyrimidine P), displays strong fluorescence signal. Dyed microspheres UV under...

Synthetic oligodeoxynucleotide is purified by capping failure sequences with an acrylated phosphoramidite followed polymerization and product extraction. The method suitable for large scale oligonucleotide drug purification.

Long oligodeoxynucleotides (ODNs) are segments of DNAs having over one hundred nucleotides (nt). They typically assembled using enzymatic methods such as PCR and ligation from shorter 20 to 60 nt ODNs produced by automated de novo chemical synthesis. While these have made many projects in areas synthetic biology protein engineering possible, they various drawbacks. For example, cannot produce genes genomes with long repeats difficulty sequences containing stable secondary structures. Here,...

Polyethylene glycol (PEG) and derivatives with eight twelve ethylene units were synthesized by stepwise addition of tetraethylene monomers on a polystyrene solid support. The monomer contains tosyl group at one end dimethoxytrityl the other. Wang resin, which 4-benzyloxy benzyl alcohol function, was used as synthetic cycle consists deprotonation, Williamson ether formation (coupling), detritylation. Cleavage PEGs from support achieved trifluoroacetic acid. synthesis including entirely...

Purification at the hundred milligram scale is as convenient sub-milligram scale. Long sequences are easily isolated from complex mixtures. The waste to product ratio reduced by hundreds of times over known methods.

The readily scalable catching by polymerization purification technology has been further advanced to purify 5′-phosphorylated synthetic oligodeoxynucleotides (ODNs). new utilizes a phosphoramidite that contains fluoride-cleavable diisopropylsilyl acetal linker and polymerizable methacrylamide group, is capable of phosphorylation ODN. For purification, the was coupled 5′-end full-length ODN on synthesizer. Because failure sequences were capped in each cycle, only phosphinylated acrylated....

A fluoride/amine-cleavable phosphoramidite designed for biotinylation, phosphorylation, and affinity purification of synthetic oligonucleotides was synthesized coupled efficiently to the 5'-end DNA on a solid-phase automatic synthesizer. The two hydroxyl groups diethyl bis(hydroxymethyl)malonate were used link biotin together through diisopropylsilyl acetal functionality phosphate ester group, respectively. cleaved from solid support fully deprotected by treating with mixture MeNH2 (∼40%)...

Abstract Several simple bismuth(III) salts were screened for the suitability to catalyze cycloisomerization of enynes. Among them, BiCl 3 gave best results. Under optimized reaction conditions, eight substrates studied, and acceptable excellent isolated yields obtained. Consistent with data in literature, electron‐deficient alkynes found be better than electron‐rich ones. Because is readily available, inexpensive environmentally benign, this soft Lewis acid catalyzed isomerization expected a...

Solid-phase synthesis of electrophilic oligodeoxynucleotides (ODNs) was achieved using dimethyl-Dmoc (dM-Dmoc) as amino protecting group. Due to the high steric hindrance 2-(propan-2-ylidene)-1,3-dithiane side product from deprotection, use excess nucleophilic scavengers such aniline prevent Michael addition deprotected ODN during cleavage and deprotection no longer needed. The improved technology demonstrated by characterization five ODNs including three modified ones. contained groups...

The cycloaddition reaction of an alkyne and azide to form a 1,2,3-triazole is widely used in many areas. However, the stability triazole moiety under mechanical stress unclear. To see if could be selectively split into presence other typical covalent bonds, mica surface functionalized with molecule containing middle activated ester at end was prepared. An atomic force microscope (AFM) tip amino groups on its ramped over predefined locations, which temporarily link through amide bond...

By use of 1,3-dithian-2-yl-methoxycarbonyl (Dmoc) as a protecting group and linker for oligodeoxynucleotide (ODN) synthesis, deprotection cleavage are achieved under non-nucleophilic oxidative conditions. The nucleophile-sensitive thioester α-chloroacetyl groups conveniently incorporated into ODN sequences. technology could be universally useful electrophilic synthesis.

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDetermination of Menthol in Peppermint Oil. Acetic Anhydride and Pyridine as ReagentJ.S. Jones S.C. FangCite this: Ind. Eng. Chem. Anal. Ed. 1946, 18, 2, 130–131Publication Date (Print):February 1, 1946Publication History Published online1 May 2002Published inissue 1 February 1946https://pubs.acs.org/doi/10.1021/i560150a016https://doi.org/10.1021/i560150a016research-articleACS PublicationsRequest reuse permissionsArticle...