A5070838814- Epigenetics and DNA Methylation
- Zebrafish Biomedical Research Applications
- Ferroptosis and cancer prognosis
- Bladder and Urothelial Cancer Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer, Hypoxia, and Metabolism
- Single-cell and spatial transcriptomics
- Urinary and Genital Oncology Studies
- Cancer Genomics and Diagnostics
- Prenatal Screening and Diagnostics
- Immune Response and Inflammation
- Pregnancy and preeclampsia studies
- Immune cells in cancer
- Neurogenesis and neuroplasticity mechanisms
- Endoplasmic Reticulum Stress and Disease
- Retinal Development and Disorders
- Ubiquitin and proteasome pathways
- Organic Electronics and Photovoltaics
- Millimeter-Wave Propagation and Modeling
- Berberine and alkaloids research
- Genomics and Chromatin Dynamics
- Reproductive System and Pregnancy
- Advanced Wireless Communication Techniques
- Autophagy in Disease and Therapy
- Semiconductor materials and devices
Zhongnan Hospital of Wuhan University
2022-2025
Wuhan University
2022-2025
Euler Technology (China)
2020-2025
Chongqing Three Gorges University
2024
Hong Kong University of Science and Technology
2002-2019
University of Hong Kong
2015-2019
National Taiwan Ocean University
2006
Shanghai Medical College of Fudan University
2006
Abstract A hallmark of tumor cells, including bladder cancer (BLCA) is metabolic reprogramming toward aerobic glycolysis (Warburg effect). The classical oncogene MYC, which crucial in regulating glycolysis, amplified and activated BLCA. However, direct targeting the c-Myc oncoprotein, regulates glycolytic metabolism, presents great challenges necessitates discovery a more clarified regulatory mechanism to develop selective targeted therapy. In this study, siRNA library deubiquitinases...
Abstract To date, most studies on the DNA polymerase, POLD1, have focused effect of POLD1 inactivation mutations in tumors. However, implications high expression tumorigenesis remains elusive. Here, we determine that has a pro-carcinogenic role bladder cancer (BLCA) and is associated to malignancy prognosis BLCA. Our demonstrate promotes proliferation metastasis BLCA via MYC. Mechanistically, stabilizes MYC manner independent its’ polymerase activity. Instead, attenuates FBXW7-mediated...
T lymphocytes are key cellular components of the adaptive immune system and play a central role in cell-mediated immunity vertebrates. Despite their heterogeneities, it is believed that all different types generated exclusively via differentiation hematopoietic stem cells (HSCs). Using temporal-spatial resolved fate-mapping analysis time-lapse imaging, here we show ventral endothelium zebrafish aorta-gonad-mesonephros posterior blood island, tissues previously known to generate HSCs...
Abstract Single-cell chromatin accessibility sequencing (scATAC-seq) reconstructs developmental trajectory by phenotypic similarity. However, inferring the exact is challenging. Previous studies showed age-associated DNA methylation (DNAm) changes in specific genomic regions, termed clock-like differential loci (ClockDML). Age-associated DNAm could either result from or at ClockDML. As cells undergo mitosis, heterogeneity of on reduced, providing a measure mitotic age. In this study, we...
Abstract Intratumor heterogeneity (ITH) of bladder cancer (BLCA) contributes to therapy resistance and immune evasion affecting clinical prognosis. The molecular cellular mechanisms contributing BLCA ITH generation remain elusive. It is found that a TM4SF1‐positive subpopulation (TPCS) can generate in BLCA, evidenced by integrative single cell atlas analysis. Extensive profiling the epigenome transcriptome all stages revealed their evolutionary trajectories. Distinct ancestor cells gave rise...
The origin of Langerhans cells (LCs), which are skin epidermis-resident macrophages, remains unclear. Current lineage tracing LCs largely relies on the promoter-Cre-LoxP system, often gives rise to contradictory conclusions with different promoters. Thus, reinvestigation an improved method is necessary. Here, using a laser-mediated temporal-spatial resolved cell labeling method, we demonstrated that most adult originated from ventral wall dorsal aorta (VDA), equivalent mouse aorta, gonads,...
Abstract Background State‐of‐art non‐invasive diagnosis processes for bladder cancer (BLCA) harbour shortcomings such as low sensitivity and specificity, unable to distinguish between high‐ (HG) low‐grade (LG) tumours, well inability differentiate muscle‐invasive (MIBC) non‐muscle‐invasive (NMIBC). This study investigates a comprehensive characterization of the entire DNA methylation (DNAm) landscape BLCA determine relevant biomarkers BLCA. Methods A total 304 samples from 224 donors were...
Abstract Background Preeclampsia, one of the most lethal pregnancy-related diseases, is associated with disruption uterine spiral artery remodeling during placentation. However, early molecular events leading to preeclampsia remain unknown. Results By analyzing placentas from preeclampsia, non-preeclampsia, and twin pregnancies selective intrauterine growth restriction, we show that pathogenesis attributed immature trophoblast maldeveloped endothelial cells. Delayed epigenetic reprogramming...
Rationale: Bladder cancer (BLCA), one of the most lethal urological tumors, exhibits high rates recurrence and chemoresistance, particularly to gemcitabine (GEM). Understanding mechanisms GEM resistance is crucial for improving therapeutic outcomes. Our study investigates role DLGAP5 in promoting through modulation glycolysis MYC protein stability BLCA cells. Methods: We utilized various cell lines clinical tissue samples analyze impact on resistance. Through biochemical assays, interaction...
Abstract Bladder cancer (BLCA) is a most common urological tumours with high rate of recurrence, which needs long‐term follow‐up. To date, diagnosis and surveillance BLCA still rely on cystoscopy, an invasive expensive method that increases the difficulty for routine Therefore, exploring new biomarkers or tests effective way to improve current clinical management BLCA. Recent years, liquid biopsy has received increasing attention, especially its great potential application in non‐invasive...
Pectolinarigenin (PEC), an active compound isolated from traditional herbal medicine, has shown potential anti-tumor properties against various types of cancer cells. However, its mechanism action in bladder (BLCA), which is one the fatal human carcinomas, remains unexplored. In this study, we first revealed that PEC, as a DNA topoisomerase II alpha (TOP2A) poison, can target TOP2A and cause significant damage. PEC induced G2/M phase cell cycle arrest via p53 pathway. Simultaneously, perform...
Bladder cancer (BLCA) is a prevalent with high case-fatality rates and substantial economic burden worldwide. Understanding its molecular underpinnings to guide clinical management crucial. Ferroptosis, recently described non-apoptotic form of cell death, initiated by the lethal accumulation iron-dependent lipid peroxidation products. Despite growing interest, roles vulnerabilities determining ferroptosis sensitivity in BLCA remain unclear. Re-analysis single-cell RNA data reveals decrease...
Highlights•mcoln1a-deficient fish display microglia impairment and aberrant neuronal activity•The activity can be rescued by expressing WT mcoln1a in microglia•Impairment of microglia-neuron contact contributes to the activitySummaryType IV mucolipidosis (ML-IV) is a neurodegenerative lysosome storage disorder caused mutations MCOLN1 gene. However, cellular molecular bases underlying phenotypes ML-IV disease remain elusive. Using forward genetic screening, we identified zebrafish mutant,...
The floating-body effect (FBE) in nonfully-depleted (NFD) SOI MOSFETs gives rise to excess low-frequency noise. For the first time, origin of noise is identified be shot associated with impact ionization current and body-source diode current. noise, normally negligible as compared flicker amplified device through FBE. model predicts that shows a Lorentzian-like spectrum verified by experimental data. physical explanation further supported coincidence corner frequency AC output impedance.
Single cell chromatin accessibility sequencing (scATAC) reconstructs developmental trajectory by phenotypic similarity. However, inferring the exact is challenging. Here, we show a simple, accurate and phenotypic-neutral measure of hierarchy -- fraction accessible clock-like loci. As cells undergo mitosis, heterogeneity on loci reduced, providing mitotic age. We developed method, EpiTrace, that counts opened from scATAC data to determine age perform lineage tracing. EpiTrace works in various...
Summary Preeclampsia, a life-threatening pregnancy complication characterized by hypertension and multiorgan damage, affects 2-5% of pregnancies causes 76,000 deaths per year. Most preeclampsia associated syndromes immediately dispel after removal placenta, indicating casual role placenta in the pathogenesis. Failed transformation spiral artery due to insufficient invasion excessive apoptosis trophoblast suggested developmental defects placenta. However, underlying molecular mechanisms that...
This study explores a multiple-input and multiple-output (MIMO) channel measurement that makes use of single-output (MISO) antenna configuration for various spacings indoor line-of-sight (LOS) Elliptical scattering propagation. With 5-element uniform linear array (ULA) transmitted system, dynamic measurements using single receiver moving over spatial distance 5 m (cong30 lambda) is considered via angular spreads plusmn9.9deg plusmn1.8deg associated with routes A B, respectively. this...