A5057136525- Epigenetics and DNA Methylation
- Bladder and Urothelial Cancer Treatments
- Ferroptosis and cancer prognosis
- Cancer, Lipids, and Metabolism
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
- Cancer Genomics and Diagnostics
- Prostate Cancer Treatment and Research
- Peroxisome Proliferator-Activated Receptors
- Cancer Immunotherapy and Biomarkers
- Renal cell carcinoma treatment
- Urinary and Genital Oncology Studies
- COVID-19 Clinical Research Studies
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Bioinformatics and Genomic Networks
- Cancer-related molecular mechanisms research
- Liver Disease Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Endoplasmic Reticulum Stress and Disease
- MicroRNA in disease regulation
- interferon and immune responses
- Microtubule and mitosis dynamics
Zhongnan Hospital of Wuhan University
2018-2025
Wuhan University
2016-2025
Wuhan Center for Disease Control and Prevention
2022
Hubei Cancer Hospital
2020
Abstract Background No clinically proven effective antiviral strategy exists for the epidemic Coronavirus Disease 2019 (COVID-19). Methods We conducted a prospective, randomized, controlled, open-label multicenter trial involving adult patients with COVID-19. Patients were randomly assigned in 1:1 ratio to receive conventional therapy plus Umifenovir (Arbidol) (200mg*3/day) or Favipiravir (1600mg*2/first day followed by 600mg*2/day) 10 days. The primary outcome was clinical recovery rate of...
Background: In addition to supportive therapy, antiviral therapy is an effective treatment for coronavirus disease 2019 (COVID-19). Objective: To compare the efficacy and safety of favipiravir umifenovir (Arbidol) treat COVID-19 patients. Methods: We conducted a prospective, randomized, controlled, open-label multicenter trial involving adult patients with COVID-19. Enrolled initial symptoms within 12 days were randomly assigned in 1:1 ratio receive conventional plus Arbidol (200 mg*3/day)...
Abstract A hallmark of tumor cells, including bladder cancer (BLCA) is metabolic reprogramming toward aerobic glycolysis (Warburg effect). The classical oncogene MYC, which crucial in regulating glycolysis, amplified and activated BLCA. However, direct targeting the c-Myc oncoprotein, regulates glycolytic metabolism, presents great challenges necessitates discovery a more clarified regulatory mechanism to develop selective targeted therapy. In this study, siRNA library deubiquitinases...
Abstract Simvastatin is currently one of the most common drugs for old patients with hyperlipidemia, hypercholesterolemia and atherosclerotic diseases by reducing cholesterol level anti-lipid properties. Importantly, simvastatin has also been reported to have anti-tumor effect, but underlying mechanism largely unknown. We collected several human bladder samples performed microarray. Data analysis suggested cancer (BCa) was significantly associated fatty acid/lipid metabolism via PPAR...
Tumor cells rely on aerobic glycolysis as their main energy resource (Warburg effect). Recent research has highlighted the importance of lipid metabolism in tumor progression, and certain cancers even turn to fatty acids fuel. Related studies have identified alterations acid human bladder cancer (BCa). Our microarray analysis showed that was activated BCa compared with normal bladder. The free (FFA) level also increased paracancerous tissues. Inhibition oxidation (FAO) etomoxir caused...
Abstract To date, most studies on the DNA polymerase, POLD1, have focused effect of POLD1 inactivation mutations in tumors. However, implications high expression tumorigenesis remains elusive. Here, we determine that has a pro-carcinogenic role bladder cancer (BLCA) and is associated to malignancy prognosis BLCA. Our demonstrate promotes proliferation metastasis BLCA via MYC. Mechanistically, stabilizes MYC manner independent its’ polymerase activity. Instead, attenuates FBXW7-mediated...
UPP1, a crucial pyrimidine metabolism-related enzyme, catalyzes the reversible phosphorylation of uridine to uracil and ribose-1-phosphate.However, effects UPP1 in bladder cancer (BLCA) have not been elucidated.AKT, which is activated mainly through dual (Thr308 Ser473), promotes tumorigenesis by phosphorylating downstream substrates.This study demonstrated that BLCA cell proliferation, migration, invasion, gemcitabine resistance activating AKT signaling pathway vitro vivo.Additionally,...
Trimethylation of histone H3K36 is a chromatin mark associated with active gene expression, which has been implicated in coupling transcription mRNA splicing and DNA damage response. SETD2 major trimethyltransferase, as tumor suppressor mammals. Here, we report the regulation protein stability by proteasome system, identification SPOP, key subunit CUL3 ubiquitin E3 ligase complex, SETD2-interacting protein. We demonstrate that SPOP critically involved control SPOP/CUL3 complex responsible...
Our study's goal was to screen novel biomarkers that could accurately predict the progression and prognosis of bladder cancer (BC). Firstly, we used Gene Expression Omnibus (GEO) dataset GSE37815 differentially expressed genes (DEGs). Secondly, DEGs construct a co-expression network by weighted gene analysis (WGCNA) in GSE71576. We then screened brown module, which significantly correlated with histologic grade (r = 0.85, p 1e-12) BC. conducted functional annotation on all module found were...
Clear cell renal carcinoma (ccRCC) is a heterogeneous tumor that the underlying molecular mechanisms are largely unclear. This study aimed to elucidate key candidate genes and pathways in ccRCC by integrated bioinformatics analysis. 1387 differentially expressed were identified based on three expression profile datasets, including 673 upregulated 714 downregulated genes. Then we used weighted correlation network analysis identify 6 modules associated with pathological stage grade, blue...
Abstract Single-cell chromatin accessibility sequencing (scATAC-seq) reconstructs developmental trajectory by phenotypic similarity. However, inferring the exact is challenging. Previous studies showed age-associated DNA methylation (DNAm) changes in specific genomic regions, termed clock-like differential loci (ClockDML). Age-associated DNAm could either result from or at ClockDML. As cells undergo mitosis, heterogeneity of on reduced, providing a measure mitotic age. In this study, we...
Abstract PRKN is a key gene involved in mitophagy Parkinson’s disease. However, recent studies have demonstrated that it also plays role the development and metastasis of several types cancers, both mitophagy-dependent mitophagy-independent manner. Despite this, potential effects underlying mechanisms Parkin on bladder cancer (BLCA) remain unknown. Therefore, this study, we investigated expression various BLCA cohorts derived from human. Here show was low acts as tumor suppressor by...
Abstract Intratumor heterogeneity (ITH) of bladder cancer (BLCA) contributes to therapy resistance and immune evasion affecting clinical prognosis. The molecular cellular mechanisms contributing BLCA ITH generation remain elusive. It is found that a TM4SF1‐positive subpopulation (TPCS) can generate in BLCA, evidenced by integrative single cell atlas analysis. Extensive profiling the epigenome transcriptome all stages revealed their evolutionary trajectories. Distinct ancestor cells gave rise...
Considering the importance of microRNAs (miRNAs) in regulating cellular processes, we performed microarray analysis and revealed miR‐4324 as one most differentially expressed miRNAs bladder cancer (BCa). Then, discovered that was a negative regulator Rac GTPase activating protein 1 (RACGAP1) RACGAP1 functioned an oncogenic BCa. Our studies indicated ectopic overexpression BCa cells significantly suppressed cell proliferation metastasis enhanced chemotherapy sensitivity to doxorubicin by...
Activation of transcription enhancers, especially super-enhancers, is one the critical epigenetic features tumorigenesis. However, very few studies have systematically identified enhancers specific in cancer tissues. Here, we studied change histone modifications MMTV-PyVT breast model, combining mass spectrometry-based proteomics and ChIP-seq-based epigenomics approaches. Some proteomic results were confirmed with western blotting IHC staining. An inhibitor H3K27ac was applied to study its...
Epidermal growth factor-containing fibulin-like extracellular matrix protein 2 (EFEMP2), an protein, is highly associated with tumor invasion and metastasis.However, influenced by the microenvironment, EFEMP2 played different roles in tumors.The current study focused on exploring role of bladder cancer (BCa).The results suggested that expression was significantly higher normal tissues cells compared BCa samples cells.And we found a negative correlation between high stage, grade, patients low...
Abstract Background There is no good prognostic model that could predict the prognosis of bladder cancer (BCa) and benefit immunotherapy. Methods Through least absolute shrinkage selection operator (LASSO) algorithm, we constructed a 13‐mRNA immune signature from TCGA cohort (n = 406). We validated its value predictive for immunotherapy with four independent validation ( GSE13507 [n 256], GSE31684 93], GSE32894 308], IMvigor210 298]). Results Our results indicating high‐risk group higher...
Abstract E3 ubiquitin ligase RNF126 (ring finger protein 126) is highly expressed in various cancers and strongly associated with tumorigenesis. However, its specific function bladder cancer (BCa) still debatable. Here, we found that was significantly upregulated BCa tissue by TCGA database, our studies indicated downregulation of inhibited cell proliferation metastasis through the EGFR/PI3K/AKT signaling pathway cells. Furthermore, identified PTEN, an inhibitor PI3K/AKT pathway, as a novel...
Abstract Background State‐of‐art non‐invasive diagnosis processes for bladder cancer (BLCA) harbour shortcomings such as low sensitivity and specificity, unable to distinguish between high‐ (HG) low‐grade (LG) tumours, well inability differentiate muscle‐invasive (MIBC) non‐muscle‐invasive (NMIBC). This study investigates a comprehensive characterization of the entire DNA methylation (DNAm) landscape BLCA determine relevant biomarkers BLCA. Methods A total 304 samples from 224 donors were...
Abstract Melatonin is a well-known natural hormone, which shows potential anticancer effect in many human cancers. Bladder cancer (BLCA) one of the most malignant cancers world. Chemoresistance an increasingly prominent phenomenon that presents obstacle to clinical treatment BLCA. There urgent need investigate novel drugs improve current status. In our study, we comprehensively explored inhibitory melatonin on BLCA and found it could suppress glycolysis process. Moreover, discovered ENO1,...
Rationale: Bladder cancer (BLCA), one of the most lethal urological tumors, exhibits high rates recurrence and chemoresistance, particularly to gemcitabine (GEM). Understanding mechanisms GEM resistance is crucial for improving therapeutic outcomes. Our study investigates role DLGAP5 in promoting through modulation glycolysis MYC protein stability BLCA cells. Methods: We utilized various cell lines clinical tissue samples analyze impact on resistance. Through biochemical assays, interaction...