A5047460689- Zebrafish Biomedical Research Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- T-cell and B-cell Immunology
- Cancer-related gene regulation
- Acute Myeloid Leukemia Research
- Ubiquitin and proteasome pathways
- Bone health and treatments
- Skin and Cellular Biology Research
- MicroRNA in disease regulation
- Bone Metabolism and Diseases
- Angiogenesis and VEGF in Cancer
- Aquaculture disease management and microbiota
- Single-cell and spatial transcriptomics
- Erythrocyte Function and Pathophysiology
- NF-κB Signaling Pathways
Hong Kong University of Science and Technology
2017-2025
University of Hong Kong
2017-2025
Peking University
2025
Peking University Shenzhen Hospital
2023-2024
T lymphocytes are key cellular components of the adaptive immune system and play a central role in cell-mediated immunity vertebrates. Despite their heterogeneities, it is believed that all different types generated exclusively via differentiation hematopoietic stem cells (HSCs). Using temporal-spatial resolved fate-mapping analysis time-lapse imaging, here we show ventral endothelium zebrafish aorta-gonad-mesonephros posterior blood island, tissues previously known to generate HSCs...
Microglia are brain-resident macrophages playing pivotal roles in CNS development and homeostasis. Yet, the cellular molecular basis governing microglia maintenance remain largely unknown. Here, via utilizing a visible conditional knockout allele of pu.1 gene (the master regulator for microglia/macrophage lineage development) to generate mosaic populations adult zebrafish, we show that while -deficient immediate viable, they less competitive chronically eliminated through Tp53-mediated cell...
Microglia are brain-resident macrophages playing pivotal roles in CNS development and homeostasis. Yet, the cellular molecular basis governing microglia maintenance remain largely unknown. Here, via utilizing a visible conditional knockout allele of pu.1 gene (the master regulator for microglia/macrophage lineage development) to generate mosaic populations adult zebrafish, we show that while -deficient immediate viable, they less competitive chronically eliminated through Tp53-mediated cell...
SignificanceHematopoietic stem cells (HSCs) are generated from specialized endothelial cells, called hemogenic (HECs). It has been debated whether HECs and non-HSC-forming conventional (cECs) arise a common precursor or represent distinct lineages. Moreover, the molecular basis underlying their fate determination is poorly understood. We use photoconvertible labeling, time-lapse imaging, single-cell RNA-sequencing analysis to trace lineage of HECs. discovered that cECs angioblast precursor,...
Corylin, a flavonoid isolated from the fruit of Psoralea corylifolia, has an osteogenic effect on osteoblasts in vitro and bone micromass ex vivo. However, mechanism corylin regulating osteoclastogenesis remain unknown. By using murine marrow macrophages as osteoclast precursor, was found to inhibit receptor activator nuclear factor (NF) κB ligand (RANKL)-induced differentiation via down-regulating osteoclastic marker genes. In parallel, F-actin formation migration were diminished...
ABSTRACT The E26 transformation-specific or E-twenty-six (ETS) genes encode a superfamily of transcription factors involved in diverse biological processes. Here, we report the identification and characterization previously unidentified member ETS factors, Spi2, that is found exclusively ray-finned fish kingdom. We show expression spi2 restricted to hemogenic endothelial cells (HECs) hematopoietic stem progenitor (HSPCs) zebrafish. Using bacteria artificial chromosome transgenesis, generate...
Microglia are the central nervous system (CNS)-resident macrophages involved in neural inflammation, neurogenesis, and activity regulation. Previous studies have shown that naturally occurring neuronal apoptosis plays a critical role regulating microglial colonization of brain zebrafish. However, molecular signaling cascades underlying apoptosis-mediated regulation these remain undefined. Here, we show basic leucine zipper (b-Zip) transcription factors, Mafba Mafbb, two zebrafish orthologs...
Abstract Microglia are brain-resident macrophages that play pivotal roles in CNS development and homeostasis. Yet, the cellular molecular basis governing microglia maintenance remain largely unknown. By using a visible conditional knockout strategy to generate mosaic zebrafish, we show deficient for Pu.1/Spi1b, master regulator development, less competitive chronically eliminated Tp53-dependent manner. Interestingly, when both Pu.1 Spi-b (the paralogue of also called Spi1a) defective,...