Dendritic cells (DCs) play an important role in anti-tumor immunity by inducing T cell differentiation. Herein, we found that the DC mechanical sensor Piezo1 stimulated stiffness or inflammatory signals directs reciprocal differentiation of TH1 and regulatory (Treg) cancer. Genetic deletion DCs inhibited generation while driving development Treg promoting cancer growth mice. Mechanistically, Piezo1-deficient regulated secretion polarizing cytokines TGFβ1 IL-12, leading to increased...

Purpose: The purpose of this study was to establish a deep learning model for automated sub-basal corneal nerve fiber (CNF) segmentation and evaluation with in vivo confocal microscopy (IVCM). Methods: A network (CNS-Net) established convolutional neural networks based on algorithm evaluation. CNS-Net trained 552 tested 139 labeled IVCM images as supervision information collected from July 2017 December 2018 Peking University Third Hospital. These were by three senior ophthalmologists ImageJ...

Abstract Purpose To investigate the impact of accommodation function on difference between cycloplegic and noncycloplegic subjective automatic refraction in adult myopes. Methods Myopic patients 18 50 years old evaluated at Peking University Third Hospital who underwent were enrolled. Accommodation function, including negative positive relative (PRA/NRA) response (binocular cross cylinder, BCC) was examined. Results Of 3268 individuals enrolled, mean age 27.3 ± 6.9 years, 34.8% participants...

Microglia are brain-resident macrophages playing pivotal roles in CNS development and homeostasis. Yet, the cellular molecular basis governing microglia maintenance remain largely unknown. Here, via utilizing a visible conditional knockout allele of pu.1 gene (the master regulator for microglia/macrophage lineage development) to generate mosaic populations adult zebrafish, we show that while -deficient immediate viable, they less competitive chronically eliminated through Tp53-mediated cell...

Microglia are brain-resident macrophages playing pivotal roles in CNS development and homeostasis. Yet, the cellular molecular basis governing microglia maintenance remain largely unknown. Here, via utilizing a visible conditional knockout allele of pu.1 gene (the master regulator for microglia/macrophage lineage development) to generate mosaic populations adult zebrafish, we show that while -deficient immediate viable, they less competitive chronically eliminated through Tp53-mediated cell...

The study aims to explore the impact of objective visual quality and retinal structure on dynamic acuity (DVA) in 30 healthy young adults with a mean age 23.0 ± 3.7 years. Binocular DVA was assessed at 20, 40, 60, 80 degrees per second (dps) habitual spectacles. Modulation transfer function (MTF), higher-order aberrations (HOA) structure, including thickness capillary density optic disc macula, were measured. differed significantly across velocities (P < 0.001). 20 dps (r =...

Our study aimed to evaluate the efficiency of intense pulsed light (IPL) combined with meibomian gland expression (MGX) in treating dysfunction (MGD).This was a prospective interventional study. A total 53 patients were included and received series three treatments at an interval 3-4 weeks. Follow-up examinations completed 4 weeks after last treatment. The Ocular Surface Disease Index (OSDI) questionnaire, tear meniscus height (TMH), break-up time (TBUT), slit-lamp examinations, vivo...

The aim of the study was to compare dynamic defocus curve on patients post-implantation extended depth-of-focus (EDOF) and monofocal intraocular lens (IOL). A total 62 age-related cataract receiving phacoemulsification with implantation TECNIS Symfony (ZXR00) or IOLs were enrolled. binocular static curves corrected distance visual acuity evaluated at one month postoperatively. ZXR00 group achieved significantly better intermediate (P = 0.044) near 0.017) (VA) than group. Two groups had...

To investigate the intraocular pressure (IOP) changes after cataract surgery, and its relationship with refractive conditions. IOP phacoemulsification lens (IOL) implantation was retrospectively reviewed. Patients were classified into 3 groups by conditions: emmetropia, mild to moderate myopia, high myopia. Basic information collected including age, sex, place of IOL, operating surgeon, conditions measured before 1, 7, 30, 90 days surgery. The study comprised 353 eyes from patients, which...

Purpose: To compare structural diameters of the ellipsoid zone (EZ) and external limiting membrane (ELM) bands on spectral domain-optical coherence tomography (SD-OCT) images between vision-improved (group A) vision-unimproved B) patients, investigate connection these parameters visual prognosis. Materials Methods: Forty-five eyes 43 patients with idiopathic full-thickness macular hole closed after vitrectomy were retrospectively reviewed. Best-corrected acuity (BCVA) SD-OCT conducted...

Understanding the impact of home confinement on axial length in myopic children undergoing orthokeratology (OK) treatment facilitates management myopia control during coronavirus disease 2019 (COVID-19) lockdown.The outbreak COVID-19 and corresponding measures have brought a considerable challenge to control. The study aimed investigate influence quarantine with OK treatment.Axial measurements before were retrospectively collected from treated OK, prospectively followed up after finishing...

Our purpose is to demonstrate the changes in cornea nerve parameters and symptoms signs dry eye disease (DED) patients after oral vitamin B1 mecobalamin treatment. In this randomized double-blind controlled trial, DED were randomly assigned either treatment group (oral mecobalamin, artificial tears) or control (artificial tears). Corneal via vivo confocal microscopy (IVCM), symptoms, assessed at baseline 1 3 months post-treatment. total, 398 eyes from 199 included. group, there significant...

To develop a deep learning model for automatically segmenting tarsus and meibomian gland areas on meibography, we included 1087 meibography images from dry eye patients. The contour of the each was labeled manually by human experts. dataset divided into training, validation, test sets. We built convolutional neural network-based U-net trained to segment area. Accuracy, sensitivity, specificity, receiver operating characteristic curve (ROC) were calculated evaluate model. area under (AUC)...

Purpose To investigate binocular dynamic visual acuity (DVA) for patients with dry eye disease (DED). Methods The prospective study included DED patients. DVA at 40 and 80 degrees per second (dps), Ocular Surface Disease Index (OSDI), tear meniscus height (TMH), film break-up time first (TBUTF), corneal fluorescein staining (CFS), eyelid margin abnormalities meibomian gland (MG) morphology function were evaluated. A deep learning model was applied to quantify the MG area proportion....

To investigate the efficacy of a novel treatment - intra-meibomian gland (MG) injection anti-VEGF agent bevacizumab for MG dysfunction (MGD) with eyelid-margin vascularity.A total 26 eyes from 13 patients diagnosed MGD and vascularity were included in our study. Patients received injections (150 μL, 2.5 mg/0.1 mL) at multiple sites 29 G needle where telangiectasia was severe. The Ocular Surface Disease Index (OSDI), tear film, tear-breakup time (TBUT), features, conjunctiva, corneal staining...

To assess the effects of oral vitamin B1 and mecobalamin on dry eye disease (DED) patient satisfaction with treatment.In this randomized controlled study, DED patients were divided into 4 groups based treatment: group 1, only artificial tears; 2, corticosteroid drops 3, B1, mecobalamin, 4, drops, tears. symptoms, signs, treatment assessed at baseline 1 2 months after total, 152 eyes from 76 (age, 55.25 ± 18.16 years) included. In there significant differences in dryness, foreign body...

To investigate the influence of different corneal refractive surgeries on dynamic visual acuity (DVA), and explore its potential factors.This was a prospective non-randomized study. Adult myopic patients undergoing bilateral laser-assisted sub-epithelial keratomileusis (LASEK), femtosecond in situ (FS-LASIK), or small incision lenticule extraction (SMILE) with Plano refraction target were enrolled. Uncorrected corrected distance (UDVA/CDVA), manifest binocular optotype-moving DVA 40 80...

To assess the efficacy of orthokeratology in controlling rate myopia progression children and investigate factors associated with axial length (AL) growth an average 48 months lens wear.As a retrospective study, 84 subjects underwent relatively complete ophthalmologic examinations. After initial wear, AL was measured on every 12 months. The linear mixed-effects model (LMM) used to compare differences rates at each time interval. contribution independent variables change assessed using...

Introduction Broken eggs are a byproduct of the poultry industry and potential nitrogen source for mushroom cultivation. However, its feasibility needs to be evaluated experimentally. Methods In this study, series different addition amounts (0, 1.8, 3.6, 5.3 8.5%, w/w) broken egg mixture (BEM) were applied in composting cultivation process oyster mushroom. The physicochemical properties bacterial communities substrate, agronomic nutritional fruiting bodies determined. Results discussion...