A5057025626- Neuroinflammation and Neurodegeneration Mechanisms
- Zebrafish Biomedical Research Applications
- Immune cells in cancer
- Pancreatic function and diabetes
- Single-cell and spatial transcriptomics
- Diabetes and associated disorders
- Diabetes Management and Research
- Neurogenesis and neuroplasticity mechanisms
- Retinal Diseases and Treatments
- Neuroscience and Neuropharmacology Research
- Metabolism, Diabetes, and Cancer
- Advanced Fluorescence Microscopy Techniques
- Calcium signaling and nucleotide metabolism
- Connexins and lens biology
- Platelet Disorders and Treatments
- Cell Image Analysis Techniques
- Sperm and Testicular Function
- Dynamics and Control of Mechanical Systems
- Immune Response and Inflammation
- Reproductive Biology and Fertility
- Vibration and Dynamic Analysis
- Image Processing Techniques and Applications
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Heat shock proteins research
Hong Kong University of Science and Technology
2015-2025
University of Hong Kong
2015-2025
Peking University
2016-2025
Shanghai Sunshine Rehabilitation Center
2024-2025
Beijing National Laboratory for Molecular Sciences
2024
Center for Life Sciences
2024
Hunan University of Traditional Chinese Medicine
2023
Peking University Shenzhen Hospital
2020-2022
Maastricht University
2021
Sichuan University
2021
The origin of Langerhans cells (LCs), which are skin epidermis-resident macrophages, remains unclear. Current lineage tracing LCs largely relies on the promoter-Cre-LoxP system, often gives rise to contradictory conclusions with different promoters. Thus, reinvestigation an improved method is necessary. Here, using a laser-mediated temporal-spatial resolved cell labeling method, we demonstrated that most adult originated from ventral wall dorsal aorta (VDA), equivalent mouse aorta, gonads,...
Our understanding on the function of microglia has been revolutionized in recent 20 years. However, process maintaining homeostasis not fully understood. In this study, we dissected features spinal repopulation following an acute partial depletion. By injecting intrathecally Mac-1-saporin, a selective immunotoxin, ablated 50% cord naive mice. Spinal repopulated rapidly and local was re-established within 14 days post-depletion. Mac-1-saporin treatment resulted cell proliferation circulating...
Congenital cataract, an ocular disease predominantly occurring within the first decade of life, is one leading causes blindness in children. However, molecular mechanisms underlying pathogenesis congenital cataract remain incompletely defined. Through whole-exome sequencing a Chinese family with we identified potential pathological variant (p.G1943E) PIKFYVE, which located PIP kinase domain PIKFYVE protein. We demonstrated that heterozygous/homozygous disruption domain, instead...
Hematopoietic development is tightly regulated by various factors. The role of RNA m6A modification during fetal hematopoiesis, particularly in megakaryopoiesis, remains unclear. Here, we demonstrate that loss methyltransferase METTL3 induces formation double-stranded RNAs (dsRNAs) and activates acute inflammation hematopoiesis. This dsRNA-mediated leads to which facilitates the generation megakaryocyte progenitors (MkP) but disrupts maturation platelet production. immune response activate...
Microglia are brain-resident macrophages playing pivotal roles in CNS development and homeostasis. Yet, the cellular molecular basis governing microglia maintenance remain largely unknown. Here, via utilizing a visible conditional knockout allele of pu.1 gene (the master regulator for microglia/macrophage lineage development) to generate mosaic populations adult zebrafish, we show that while -deficient immediate viable, they less competitive chronically eliminated through Tp53-mediated cell...
Microglia are brain-resident macrophages playing pivotal roles in CNS development and homeostasis. Yet, the cellular molecular basis governing microglia maintenance remain largely unknown. Here, via utilizing a visible conditional knockout allele of pu.1 gene (the master regulator for microglia/macrophage lineage development) to generate mosaic populations adult zebrafish, we show that while -deficient immediate viable, they less competitive chronically eliminated through Tp53-mediated cell...
How pancreatic β-cells acquire function in vivo is a long-standing mystery due to the lack of technology visualize β-cell living animals. Here, we applied high-resolution two-photon light-sheet microscope for first imaging Ca2+activity every Tg (ins:Rcamp1.07) zebrafish. We reveal that heterogeneity functional development occurred as two waves propagating from islet mantle core, coordinated by vascularization. Increasing amounts glucose induced acquisition and enhancement via activating...
Heterogeneity broadly exists in various cell types both during development and at homeostasis. Investigating heterogeneity is crucial for comprehensively understanding the complexity of ontogeny, dynamics, function specific types. Traditional bulk-labeling techniques are incompetent to dissect within population, while new single-cell lineage tracing methodologies invented last decade can hardly achieve high-fidelity labeling long-term in-vivo observation simultaneously. In this work, we...
Microglia are tissue-resident macrophages in the central nervous system (CNS) that play essential roles regulation of CNS development and homeostasis. Yet, genetic networks governing microglia remain incompletely defined. Here, we report identification characterization a microglia-defective zebrafish mutant wulonghkz12 (wulhkz12) isolated from an ethylnitrosourea (ENU)-based screen. We show wulhkz12 mutants harbors missense point mutation gene region encoding PRY/SPRY domain tripartite-motif...
Background: Age-related macular degeneration (AMD) is the most common cause of visual disorders in aged population and characterized by formation retinal pigment epithelium (RPE) deposits dysfunction/death RPE photoreceptors. It supposed that both oxidative stress inflammation play a critical role pathogenesis AMD. The development therapeutic strategies against AMD urgently needed. Rubus suavissimus S. Lee (RS), medicinal plant growing southwest region China, has been used as an herbal tea...
Abstract The dynamic behaviors of large-scale hoop truss antennas (HTAs) are significantly influenced by the nonlinear torque transmission properties flexible hinges. Due to nonlinearity hinge, HTR can be easily excited exhibit internal resonances that result in energy exchanging between adjacent two or three modes. This paper focuses on 3:1 resonant response an articulated induced hinge. analytical modes obtained using global mode method (GMM), and validated finite element (FEM). Then...
Abstract Microglia are brain-resident macrophages that play pivotal roles in CNS development and homeostasis. Yet, the cellular molecular basis governing microglia maintenance remain largely unknown. By using a visible conditional knockout strategy to generate mosaic zebrafish, we show deficient for Pu.1/Spi1b, master regulator development, less competitive chronically eliminated Tp53-dependent manner. Interestingly, when both Pu.1 Spi-b (the paralogue of also called Spi1a) defective,...
Here we have developed a novel large-field high-resolution two-photon digital scanned light-sheet microscopy (2P3A-DSLM) based on ultrafast axial scanning of illumination focal spot with tunable acoustic gradient (TAG) index device. This new technique provides sub-micron resolution large field view in deep tissues. In vivo imaging 2P3A-DSLM resolves functional maturation pancreatic beta cells and neuron circuit activities live Zebrafish embryos.
Abstract The insulin-secreting cells generated from stem in vitro are less glucose responsive than primary β-cells. To search for the missing ingredients that needed β-cell maturation, we have longitudinally monitored function of every Tg ( ins:Rcamp1.07 ) zebrafish embryos with a newly-invented two-photon light-sheet microscope. We shown maturation begins islet mantle and propagates to core during hatching period, coordinated by vascularization. Lower concentration is optimal initiate while...
Cell fate mapping and lineage tracing are significant ways to understanding the developmental origins of biological tissues. It requires labeling individual cells development their progeny. We develop an infrared laser-evoked gene operator heat-shock microscope system achieve single-cell in zebrafish. With a fluorescent thermometry technique, we measure temperature increase zebrafish tissues induced by laser identify optimal heat shock conditions for induction different types cells. use this...