A5017586234- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- Liver Disease Diagnosis and Treatment
- Autophagy in Disease and Therapy
- Control Systems and Identification
- Advanced Control Systems Optimization
- Adipose Tissue and Metabolism
- Fault Detection and Control Systems
- interferon and immune responses
- Stability and Control of Uncertain Systems
- Andrographolide Research and Applications
- Adaptive Control of Nonlinear Systems
- Diabetes and associated disorders
- Immune Cell Function and Interaction
- PARP inhibition in cancer therapy
- Advanced Algorithms and Applications
- Diet, Metabolism, and Disease
- Pituitary Gland Disorders and Treatments
- Glioma Diagnosis and Treatment
- Adipokines, Inflammation, and Metabolic Diseases
- Ubiquitin and proteasome pathways
- Grey System Theory Applications
- Connective Tissue Growth Factor Research
- Immunotherapy and Immune Responses
- Phytochemistry and Bioactive Compounds
Third Affiliated Hospital of Sun Yat-sen University
2020-2025
Sun Yat-sen University
2020-2025
Guangzhou University
2025
Sun Yat-sen University Cancer Center
2023-2025
Peking University
2024
Center for Life Sciences
2024
Heilongjiang Bayi Agricultural University
2010-2024
University of Nottingham Ningbo China
2024
Key Laboratory of Guangdong Province
2023
University of Michigan–Ann Arbor
2017-2020
West Nile virus (WNV) causes an acute neurological infection attended by massive neuronal cell death. However, the mechanism(s) behind virus-induced death is poorly understood. Using a library containing 77,406 sgRNAs targeting 20,121 genes, we performed genome-wide screen followed second with sub-library. Among genes identified, seven EMC2, EMC3, SEL1L, DERL2, UBE2G2, UBE2J1, and HRD1, stood out as having strongest phenotype, whose knockout conferred strong protection against WNV-induced...
Significance This study provides insights into the physiological role of Sel1L, an adaptor protein for ubiquitin ligase Hrd1 in endoplasmic reticulum-associated degradation (ERAD). Using both animal and cell models, this unequivocal evidence indispensable Sel1L stabilization, mammalian ERAD, reticulum homeostasis, translation, cellular organismal survival. Moreover, generation inducible knockout mouse models deficient unprecedented opportunity to elucidate functional importance key branch...
Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) protect against diabetic cardiovascular diseases and nephropathy. However, their activity in retinopathy (DR) remains unclear. Our retrospective cohort study involving 1626 T2DM patients revealed superior efficacy of GLP-1 RAs controlling DR compared to other glucose-lowering medications, suggesting advantage treatment. By single-cell RNA-sequencing analysis immunostaining, we observed a high expression GLP-1R retinal endothelial cells,...
<h3>Background</h3> Non-alcoholic fatty liver disease (NAFLD) is characterised by accumulation of excessive triglycerides in the liver. Obesity usually associated with NAFLD through an unknown mechanism. <h3>Objective</h3> To investigate roles Yin Yang 1 (YY1) progression obesity-associated hepatosteatosis. <h3>Methods</h3> Expression levels hepatic YY1 were identified microarray analysis high-fat-diet (HFD)-induced obese mice. Liver triglyceride metabolism was analysed mice overexpression...
Peptide hormones are crucial regulators of many aspects human physiology. Mutations that alter these signaling peptides associated with physiological imbalances underlie diseases. However, the conformational maturation peptide hormone precursors (prohormones) in ER remains largely unexplored. Here, we report proAVP, precursor for antidiuretic arginine-vasopressin, within requires ER-associated degradation (ERAD) activity Sel1L-Hrd1 protein complex. Serum hyperosmolality induces expression...
Pro-opiomelanocortin (POMC) neurons function as key regulators of metabolism and physiology by releasing prohormone-derived neuropeptides with distinct biological activities. However, our understanding early events in prohormone maturation the ER remains incomplete. Highlighting significance this gap knowledge, a single POMC cysteine-to-phenylalanine mutation at position 28 (POMC-C28F) is defective for processing causes onset obesity dominant-negative manner humans through an unclear...
Atherosclerosis is the major contributor to cardiovascular mortality worldwide. Alternate day fasting (ADF) has gained growing attention due its metabolic benefits. However, effects of ADF on atherosclerotic plaque formation remain inconsistent and controversial in animal models. The present study was designed investigate atherosclerosis apolipoprotein E-deficient (Apoe -/- ) mice. Eleven-week-old male Apoe mice fed with Western diet (WD) were randomly grouped into ad libitum (AL) group...
Megakaryocytes (MKs) continuously produce platelets to support hemostasis and form a niche for hematopoietic stem cell maintenance in the bone marrow. MKs are also involved inflammatory responses; however, mechanism remains poorly understood. Using single-cell sequencing, we identified CXCR4 highly expressed MK subpopulation, which exhibited both MK-specific immune characteristics. high interacted with myeloid cells promote their migration stimulate bacterial phagocytosis of macrophages...
Proteotoxicity induced by misfolded or aggregated proteins causes progressive neuronal damage. The endoplasmic reticulum (ER) protein quality control (ERQC) pathways are responsible for mitigating the accumulation of these proteins, thus reducing proteotoxicity. Enhancing ERQC is a promising strategy treating neurodegenerative diseases. However, mechanisms governing initiation and degradation in neurons remain largely unknown vivo. In studying maturation proAVP mouse AVP neurons, this study...
Background: Chronic inflammation in type 2 diabetes mellitus (T2DM) is an essential contributor to the development of diabetic retinopathy (DR). The monocyte–to–high-density lipoprotein cholesterol (HDL-C) ratio (MHR) a novel and simple measure related inflammatory oxidative stress status. However, little known regarding role MHR evaluating DR. Methods: A total 771 patients with T2DM 607 healthy controls were enrolled this cross-sectional study. determination eye examination performed....
Andrographolide has been reported to possess a variety of pharmacological activities. In this study, we have investigated the effect andrographolide on production TNF-α and IL-12 (Interleukin-12) in murine peritoneal macrophages. decreased TNF-α, IL-12a IL-12b at mRNA level, reduced IL-12p70 proteins concentration-dependent manner. Furthermore, found that addition inhibited activation ERK1/2 MAP kinase, but not JNK, p38 or NF-κB. These results suggested inhibit LPS-induced via suppression...
Disturbed endoplasmic reticulum (ER) stress response driven by the excessive lipid accumulation in liver is a characteristic feature pathogenesis of non-alcoholic fatty disease (NAFLD). Restoring metabolic homeostasis targeting ER potentially therapeutic strategy for NAFLD. Here we aim to identify novel proteins or pathways involved regulating and targets alleviating Proteomic transcriptomic analysis demonstrated that major urinary (MUPs) were significantly reduced livers from NAFLD mouse...
Brown adipose tissue (BAT) is the main site of nonshivering thermogenesis which plays an important role in and energy metabolism. However, regulatory factors that inhibit BAT activity remain largely unknown. Here, cardiotrophin-like cytokine factor 1 (CLCF1) identified as a negative regulator BAT. Adenovirus-mediated overexpression CLCF1 greatly impairs thermogenic capacity reduces metabolic rate. Consistently, BAT-specific ablation enhances function expenditure under both thermoneutral cold...
Diabetic retinopathy (DR) is a major cause of blindness and characterized by dysfunction the retinal microvasculature. Neutrophil stasis, resulting in inflammation occlusion microvessels, key mechanism driving DR. These plugging neutrophils subsequently release neutrophil extracellular traps (NETs), which further disrupts vasculature. Nevertheless, primary catalyst for NETs extrusion microenvironment under diabetic conditions remains unidentified. In recent studies, cellular communication...