A5075164750- MicroRNA in disease regulation
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Video Surveillance and Tracking Methods
- Mosquito-borne diseases and control
- Impact of Light on Environment and Health
- Automated Road and Building Extraction
- Optical Systems and Laser Technology
- Nanoparticles: synthesis and applications
- Vector-borne infectious diseases
- Viral Infections and Vectors
- Laser Material Processing Techniques
- Congenital heart defects research
- Surface Roughness and Optical Measurements
- Electrochemical Analysis and Applications
- RNA Interference and Gene Delivery
- Genomics and Phylogenetic Studies
- Insect symbiosis and bacterial influences
- Advanced Neural Network Applications
- Environmental Toxicology and Ecotoxicology
- Cardiac Valve Diseases and Treatments
- Cardiac Fibrosis and Remodeling
- RNA regulation and disease
Stanford University
1995-2014
Palm Beach Gardens Medical Center
2014
Texas Tech University
2013
Wuhan Institute of Virology
2010-2011
Chinese Academy of Sciences
2011
West Nile virus (WNV) causes an acute neurological infection attended by massive neuronal cell death. However, the mechanism(s) behind virus-induced death is poorly understood. Using a library containing 77,406 sgRNAs targeting 20,121 genes, we performed genome-wide screen followed second with sub-library. Among genes identified, seven EMC2, EMC3, SEL1L, DERL2, UBE2G2, UBE2J1, and HRD1, stood out as having strongest phenotype, whose knockout conferred strong protection against WNV-induced...
Abstract Background MicroRNAs are a family of ~22 nt small RNAs that can regulate gene expression at the post-transcriptional level. Identification these molecules and their targets aid understanding regulatory processes. Recently, HTS has become common identification method but there two major limitations associated with technique. Firstly, low efficiency, typically less than 1 in 10,000 sequences representing miRNA reads secondly preferentially highly expressed miRNAs. If available,...
Japanese encephalitis virus (JEV), a mosquito-borne zoonotic pathogen, is one of the major causes viral worldwide. Previous phylogenetic studies based on envelope protein indicated that there are four genotypes, and surveillance data suggest genotype I gradually replacing III as dominant strain. Here we report an evolutionary analysis 98 full-length genome sequences JEV, including 67 new samples isolated from humans, pigs, mosquitoes, midges. bats in affected areas. To investigate...
Pol III promoters such as U6 are commonly used to express small RNAs, including interfering RNA, short hairpin and guide for the clustered regularly interspaced palindromic repeats genome-editing system. However, whether RNAs were precisely expressed desired has not been studied. Here, using deep sequencing analyze we show that, mouse promoter, sequences immediately upstream of putative initiation site, which is often modified accommodate restriction enzyme sites that enable easy cloning...
Microprocessor [Drosha-DGCR8 (DiGeorge syndrome critical region gene 8) complex] processing of primary microRNA (pri-miRNA) is the first step in miRNA biogenesis, but how Drosha cleavage site determined has been unclear. Previous models proposed that Drosha-DGCR8 complex measures either ~22 nt from upper stem-single-stranded RNA (ssRNA, terminal loop) junction or ~11 lower stem-ssRNA to determine site. Here, using miRNA-offset RNAs site, we show distances both and junctions human cells,...
Heart valve disease affects up to 30% of the population and has been shown have origins during embryonic development. Valvulogenesis begins with formation endocardial cushions in atrioventricular canal outflow tract regions. Subsequently, remodel, elongate progressively form mature structures composed a highly organized connective tissue that provides necessary biomechanical function throughout life. While cushion well studied, processes required for remodeling are less understood. The...
For the visible-infrared person re-identification (VI-ReID) task, one of primary challenges lies in significant cross-modality discrepancy. Existing methods struggle to conduct modality-invariant information mining. They often focus solely on mining singular dimensions like spatial or channel, and overlook extraction specific-modality multi-dimension information. To fully mine across a wide range, we introduce Wide-Ranging Information Mining Network (WRIM-Net), which mainly comprises...
miRPara, is a new software tool that predicts mature miRNA in species specific manner based on three SVM trained models from comprehensive set of different parameters related to the physical properties pre-miRNA and its miRNAs. It achieves an accuracy up 80% for animal virus sequences against experimentally verified miRNAs predicted long genome sequences, making it one most accurate methods available. Because greater diversity plant miRNAs, only 70% was achieved, but this nevertheless more...