A5101571630- Polymer composites and self-healing
- CRISPR and Genetic Engineering
- Telomeres, Telomerase, and Senescence
- biodegradable polymer synthesis and properties
- Biotin and Related Studies
- Natural Fiber Reinforced Composites
- Neuroinflammation and Neurodegeneration Mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Pluripotent Stem Cells Research
- Ubiquitin and proteasome pathways
- Polymer Foaming and Composites
- RNA modifications and cancer
- Neurogenesis and neuroplasticity mechanisms
- Sirtuins and Resveratrol in Medicine
- Advanced biosensing and bioanalysis techniques
- Fuel Cells and Related Materials
- Gut microbiota and health
- Lysosomal Storage Disorders Research
- Hormonal and reproductive studies
- Immune cells in cancer
- Gynecological conditions and treatments
- Stress Responses and Cortisol
- Biochemical effects in animals
- Parathyroid Disorders and Treatments
- NF-κB Signaling Pathways
Sun Yat-sen Memorial Hospital
2022-2025
Sun Yat-sen University
2012-2025
Inner Mongolia Agricultural University
2024
Third Xiangya Hospital
2024
Central South University
2023-2024
Fourth Hospital of Changsha
2024
Hunan Provincial Center for Disease Control and Prevention
2024
Yangzhou University
2024
First Affiliated Hospital of Jiangxi Medical College
2023
Jiangxi Provincial People's Hospital
2023
Intrauterine adhesion (IUA) is a common uterine cavity disease which can be caused by mechanical damage that may eventually lead to infertility and pregnancy abnormalities. Since the effect of therapeutic drugs appears disappointing, cell therapy has emerged as an alternative choice for endometrium regeneration. The aim this study investigate whether combination hydrogel Pluronic F-127 (PF-127), Vitamin C (Vc), bone marrow stromal (BMSC) mixture could feasible strategy improve endometrial...
Abstract Rheb is a small G protein that functions as the direct activator of mechanistic target rapamycin complex 1 (mTORC1) to coordinate signaling cascades in response nutrients and growth factors. Despite extensive studies, guanine nucleotide exchange factor (GEF) directly activates remains unclear, at least part due dynamic transient nature protein–protein interactions (PPIs) are hallmarks signal transduction. Here, we report development rapid robust proximity labeling system named...
The glutamate pathways are involved in diverse processes such as learning and memory, epilepsy, they play important roles neural plasticity, development, neurodegeneration. It has been proposed that autism could be a hypoglutamatergic disorder. Recently, Jamain et al. reported the receptor 6 (GluR6 or GRIK2) is linkage disequilibrium with autism. In present study, transmission test (TDT) haplotype were performed to analyze four SNPs (SNP1: rs995640; SNP2: rs2227281; SNP3: rs2227283; SNP4:...
Proteotoxicity induced by misfolded or aggregated proteins causes progressive neuronal damage. The endoplasmic reticulum (ER) protein quality control (ERQC) pathways are responsible for mitigating the accumulation of these proteins, thus reducing proteotoxicity. Enhancing ERQC is a promising strategy treating neurodegenerative diseases. However, mechanisms governing initiation and degradation in neurons remain largely unknown vivo. In studying maturation proAVP mouse AVP neurons, this study...
Acrylated epoxidized soybean oil was chemically grafted with difunctional flame retardants carrying phosphorus, double bonds or biphenyl groups, and then copolymerized styrene to produce bio-foams high mechanical properties intrinsic resistance. Owing the introduction of rigid comonomer, additional side showed compressive strength similar that conventional unsaturated polyester foam, antiflaming capability biodegradability as well. More importantly, modified retardant partially took role...
Abstract LPS is the known component of bacterial pathogens that stimulates a number proinflammatory factors. However, mechanism induction these factors by has not been fully elucidated. We show here induces retinoic acid-inducible gene-I (RIG-I) in vitro and vivo as result from autocrine secretion IFN-β macrophages. TIR-domain-containing adapter-inducing IFN-β-deficient mouse embryo fibroblast (trif−/−) fail to expression RIG-I following stimulation. Interference short interfering RNA...
We previously found that excessive ethanol drinking activates Fyn in the dorsomedial striatum (DMS) (Wang et al., 2010; Gibb 2011). Ethanol-mediated activation DMS leads to phosphorylation of GluN2B subunit NMDA receptor, enhancement channel's activity, and development and/or maintenance behaviors 2007, 2010). Protein tyrosine phosphatase α (PTPα) is essential for kinase (Bhandari 1998), we showed ethanol-mediated facilitated by recruitment PTPα synaptic membranes, compartment where resides...
Abstract The ST riatal‐Enriched protein tyrosine Phosphatase 61 ( STEP ) inhibits the activity of kinase Fyn and dephosphorylates GluN2B subunit NMDA receptor, whereas A phosphorylation phosphatase (Pharmacol. Rev. , 64, p. 65). Previously, we found that ethanol activates in dorsomedial striatum DMS leading to phosphorylation, which, turn, underlies development intake J. Neurosci., 30, 10187). Here, tested hypothesis inhibition by is upstream Fyn/GluN2B. We show exposure mice increased which...
Abstract Background The aim of this study was to examine the characteristics diurnal cortisol rhythm in childhood obesity and its relationships with anthropometry, pubertal stage physical activity. Methods Thirty-five children (median age: 11.80[interquartile range 10.30, 13.30] median BMI z-score: 3.21[interquartile 2.69, 3.71]) 22 normal weight 10.85[interquartile 8.98, 12.13] − 0.27[interquartile 0.88, 0.35]) were recruited. Saliva samples collected at 08:00, 16:00 23:00 h. Cortisol...
Introduction: The early diagnosis of kidney injury in type 2 diabetes (T2DM) is important to prevent the long-term damaging effects loss and decisive for patient outcomes. While SIRT2 implicated pathogenesis, its correlation with diabetic nephropathy remains unexplored. This study was designed evaluate association urine levels injury, as well potential underlying mechanisms. Methods: In T2DM patients, db/db mice, high glucose plus palmitic acid treated HK2 cell models, ELISA,...
Shwachman-Diamond syndrome (SDS) is a rare pediatric disease characterized by various systemic disorders, including hematopoietic dysfunction. The mutation of Shwachman-Bodian-Diamond (SBDS) gene has been proposed to be major causative reason for SDS. Although SBDS patients were reported have shorter telomere length in granulocytes, the underlying mechanism still unclear. Here we provide data elucidate role protection. We demonstrate that deficiency leads shortening. found overexpression...
Most tumor cells reactivate telomerase to ensure unlimited proliferation, whereas the expression of human reverse transcriptase (hTERT) is tightly regulated and rate-limiting for activity maintenance. Several general transcription factors (TFs) have been found in regulating hTERT transcription; however, a systematic study lacking. Here we performed an inducible CRISPR/Cas9 KO screen using core promoter-driven reporter. We identified numerous positive regulators including E3 ligase DTX2. In...
Abstract BACKGROUND: Biofoam composites were prepared using short sisal fibers as reinforcement and acrylated epoxidized soybean oil matrix, aiming at replacing traditional unsaturated polyester foams in structural applications. The compressive properties of the examined a function fiber loading, length foam density. RESULTS: composite with 10 phr (parts per hundred base resin by weight) possessed similar to those commercial foams. A study failure mechanism revealed that debonding between...
Abstract Eupafolin is a phyto compound of flavone that exerts anti‐inflammatory, antioxidant, and antiproliferative properties. The main purpose this study to examine the antidiabetic effect eupafolin on nicotinamide–streptozotocin (STZ)‐induced Type 2 diabetes (T2D) rats. After nicotinamide (120 mg/kg) treatment, STZ (60 was administrated intravenously induce T2D. Rats with fasting blood glucose (FBG) > 200 mg/dL are chosen for 7 days after T2D induction. treatment continued another 15...
The tyrosine kinase Fyn plays an important role in synaptic plasticity, learning, and memory. Here we report that is activated response to 15 minutes D1 receptor (D1R) but not D2 (D2R) stimulation specifically the dorsomedial striatum (DMS) of mice other substriatal regions, dorsolateral (DLS) nucleus accumbens (NAc). Once phosphorylates its substrate GluN2B, show GluN2B phosphorylated only DMS striatal regions. Striatal neurons are divided into D1R expressing medium spiny (MSNs) D2R MSNs....
The reduction of protein translation is a common feature in senescent cells and aging organisms, yet the underlying mechanisms are not fully understood. Here we show that both global mRNA mammalian/mechanistic target rapamycin complex 1 (mTORC1) kinase activity declined model mouse embryonic fibroblasts (MEFs). Furthermore, RNA-seq analyses from polysomal versus total fractions identify TOP-like Rps15 whose regulated by mTORC1 during MEF senescence. Overexpression delays senescence, possibly...
Objective: Mutations in the ACAN gene have been reported to cause short stature. However, prevalence estimates of pathogenic variants individuals with stature vary, and correlation between genotype clinical phenotype remain be evaluated. To determine among Chinese people analyze relationship main manifestations advanced bone age patients variants. Methods: We performed next-generation sequencing-based genetic analyses on 442 were summarized, previously cases retrospectively analyzed, an...
Abstract Background Embryonic stem cells (ESCs) are important source of clinical for therapy, so dissecting the functional gene regulatory network involved in their self-renewal and proliferation is an urgent task. We previously reported that Ddx56 interacts with core transcriptional factor Oct4 by mass spectrometry analysis ESCs. However, exact function ESCs remains unclear. Methods investigated role mouse (mESCs) through both gain- loss-of-function strategies. The effect on mESCs was...
To produce soybean oil based foam plastics in terms of the supercritical carbon dioxide foaming technique, B-stage gel formed by radical copolymerization acrylated epoxidized (AESO) and styrene (St) was prepared studied. Accordingly, a dual initiator system consisting cyclohexanone peroxide (CP) di-tert-butyl (DTBP) catalyzed cobalt octoate (CoOct) introduced, which provided with (i) good permeability sufficient thermal stability for saturation (ii) polymerizability freezing depressurization...
Stem cell exhaustion is a hallmark of aging. Klotho-deficient mice (kl/kl mice) murine model that mimics human aging with significant bone abnormalities. The aim this study using kl/kl to investigate the functional change marrow-derived mesenchymal stem cells (BMSCs) and explore underlying mechanism. We found klotho deficiency leads In addition, BMSCs manifested hyperactive proliferation but functionally declined both in vivo vitro. Mammalian target rapamycin complex 1 (mTORC1) activity was...
We have recently established that phosphocreatine (PCr), a high-energy phosphate compound known to serve as an intracellular energy reserve, accumulates in the seminal vesicular fluid (SVF) of mouse and rat. To investigate whether accumulation PCr extracellular vesicles mice is androgen-dependent, young adult (Swiss Webster, 6–7 wk old) were orchidectomized. Involution following orchidectomy resulted total absence SVF. Administration testosterone propionate (TP) 2 later at daily dose 5 µ/g...