A5024901184- Regulation of Appetite and Obesity
- Biochemical Analysis and Sensing Techniques
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Herpesvirus Infections and Treatments
- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Protein Kinase Regulation and GTPase Signaling
- Virus-based gene therapy research
- Cytokine Signaling Pathways and Interactions
- Animal Virus Infections Studies
- Sleep and Wakefulness Research
- Hypothalamic control of reproductive hormones
- Cytomegalovirus and herpesvirus research
- Diet and metabolism studies
- Receptor Mechanisms and Signaling
- PI3K/AKT/mTOR signaling in cancer
- Diabetes and associated disorders
- Neuroendocrine regulation and behavior
- Ovarian function and disorders
- Neuropeptides and Animal Physiology
- Poxvirus research and outbreaks
- Vaccine Coverage and Hesitancy
- Toxin Mechanisms and Immunotoxins
- GDF15 and Related Biomarkers
University of Michigan
2016-2025
Diabetes Australia
2007-2024
ORCID
2024
Ann Arbor Center for Independent Living
2020-2023
Laboratoire d’immunologie intégrative du cancer
2009-2022
James A. Haley Veterans' Hospital
2022
University of South Florida
2022
Lancashire Teaching Hospitals NHS Foundation Trust
2013-2022
Michigan Medicine
2008-2021
The University of Texas Medical Branch at Galveston
2003-2014
The adipocyte-derived hormone leptin signals the status of body energy stores by activating long form receptor (LRb). Activation LRb results in activation associated Jak2 tyrosine kinase and transmission downstream phosphotyrosine-dependent signals. We have investigated signaling function mutant intracellular domains under control extracellular erythropoietin (Epo) receptor. By using this system, we confirm that two residues domain murine become phosphorylated to mediate signaling;...
During leptin signaling, each of the phosphorylated tyrosine residues on long form receptor (LRb) mediates distinct signals. Phosphorylated Tyr<sup>1138</sup> binds STAT3 to mediate its phosphorylation and transcriptional activation, while Tyr<sup>985</sup> phosphatase SHP-2 reportedly both activation ERK kinases inhibition LRb-mediated activation. We show here that although mutation does not alter signaling by erythropoietin receptor-LRb (ELR) chimeras in transfected 293 cells at short...
In peripheral tissues, insulin signaling involves activation of the receptor substrate (IRS)-phosphatidylinositol 3-kinase (PI3K) enzyme system. hypothalamus, functions with leptin as an afferent adiposity signal important for regulation body fat stores and hepatic glucose metabolism. To test hypothesis that hypothalamic action intracellular PI3K signaling, we used histochemical biochemical methods to determine effect on IRS-PI3K activity. Here, report induces tyrosine phosphorylation IRS-1...
IRS-1 (insulin receptor substrate 1) is a principal insulin that undergoes tyrosine phosphorylation during stimulation. It contains over 20 potential sites, and we suspect multiple signals are enabled when the activated kinase phosphorylates several of them. Tyrosine-phosphorylated binds specifically to various cellular proteins containing Src homology 2 (SH2) domains (SH2 proteins). We identified some residues undergo insulin-stimulated by purified in intact cells Automated sequencing...
Although several interleukin-3 (IL-3)-dependent cell lines proliferate in response to IL-4 or insulin, the 32D line does not. Insulin and sensitivity was restored cells by expression of IRS-1, principal substrate insulin receptor. possessed receptors for both factors, they lacked IRS-1--related protein, 4PS, which becomes phosphorylated tyrosine insulin- IL-4--responsive after stimulation. These results indicate that factors bind unrelated can use similar mitogenic signaling pathways...
Two known types of leptin-responsive neurons reside within the arcuate nucleus: agouti gene-related peptide (AgRP)/neuropeptide Y (NPY) neuron and proopiomelanocortin (POMC) neuron. By deleting leptin receptor gene (Lepr) specifically in AgRP/NPY and/or POMC mice, we examined several combined contributions these to action. Body weight adiposity were increased by Lepr deletion from AgRP individually, simultaneous both (A+P LEPR-KO mice) further measures. Young (periweaning) A+P mice exhibit...
OBJECTIVE Conditional gene targeting has been extensively used for in vivo analysis of function β-cell biology. The objective this study was to examine whether mouse transgenic Cre lines, mediate β-cell– or pancreas-specific recombination, also drive expression the brain. RESEARCH DESIGN AND METHODS Transgenic lines driven by Ins1, Ins2, and Pdx1 promoters were bred R26R reporter strains. activity assessed β-galactosidase yellow fluorescent protein pancreas Endogenous monitored using...