A5101845866- Hematopoietic Stem Cell Transplantation
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Cytomegalovirus and herpesvirus research
- Immunodeficiency and Autoimmune Disorders
- Natural product bioactivities and synthesis
- Cancer-related gene regulation
- Circular RNAs in diseases
- Wnt/β-catenin signaling in development and cancer
- CAR-T cell therapy research
- Protein Tyrosine Phosphatases
- Endoplasmic Reticulum Stress and Disease
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Bioactive Compounds and Antitumor Agents
- Nuclear Physics and Applications
- Genetic and Kidney Cyst Diseases
- RNA Interference and Gene Delivery
- Carbon and Quantum Dots Applications
- RNA Research and Splicing
- Polyomavirus and related diseases
- Parvovirus B19 Infection Studies
- Nanocluster Synthesis and Applications
Tianjin University
2019-2025
Renmin Hospital of Wuhan University
2008-2025
Wuhan University
2008-2025
University of Pennsylvania
2014-2024
Children's Hospital of Philadelphia
2013-2024
Northeastern University
2024
Jining Medical University
2024
China University of Petroleum, Beijing
2024
Gene Therapy Laboratory
2023
Xi'an Jiaotong University
2023
Disturbances in protein folding and membrane compositions the endoplasmic reticulum (ER) elicit unfolded response (UPR). Each of three UPR sensory proteins-PERK (PEK/EIF2AK3), IRE1, ATF6-is activated by ER stress. PERK phosphorylation eIF2 represses global synthesis, lowering influx nascent polypeptides into stressed ER, coincident with preferential translation ATF4 (CREB2). In cultured cells, induces transcriptional expression genes directed arm UPR, including involved amino acid...
Hematopoietic stem cells (HSCs) are the source of all blood over an individual's lifetime. Diseased HSCs can be replaced with gene-engineered or healthy through HSC transplantation (HSCT). However, current protocols carry major side effects and have limited access. We developed CD117/LNP-messenger RNA (mRNA), a lipid nanoparticle (LNP) that encapsulates mRNA is targeted to cell factor receptor (CD117) on HSCs. Delivery anti-human CD117/LNP-based editing system yielded near-complete...
We have designed and synthesized 16 new olean- urs-1-en-3-one triterpenoids with various modified rings C as potential antiinflammatory cancer chemopreventive agents evaluated their inhibitory activities against production of nitric oxide induced by interferon-γ in mouse macrophages. This investigation revealed that 9(11)-en-12-one 12-en-11-one functionalities ring increase the potency about 2−10 times compared original 12-ene. Subsequently, we novel derivatives nitrile carboxyl groups at...
New derivatives with electron-withdrawing substituents at the C-2 position of 3-oxoolean-1-en-28-oic acid were synthesized. Among them, 2-cyano-3,12-dioxoolean-1,9-dien-28-oic (CDDO) was 400 times more potent than previous compounds we have made as an inhibitor production nitric oxide induced by interferon-γ in mouse macrophages (IC50, 0.4 nM). The potency CDDO similar to that dexamethasone, although does not act through glucocorticoid receptor.
Neither the mechanisms that govern lip morphogenesis nor cause of cleft are well understood. We report genetic inactivation Lrp6, a co-receptor Wnt/beta-catenin signaling pathway, leads to with palate. The activity Wnt reporter is blocked in orofacial primordia by Lrp6 deletion mice. morphological dynamic required for normal formation and fusion disrupted these mutants. expression homeobox genes Msx1 Msx2 dramatically reduced mutants, which prevents outgrowth primordia, especially site....
Skin fibrosis, characterized by excessive accumulation of extracellular matrix (ECM) in the dermis, can lead to hypertrophic scars and impaired mobility. The ErbB family receptor tyrosine kinases, including ErbB1 ErbB2, plays a crucial role organ but their specific impact on skin fibrosis is less understood. This study investigated ErbB2 therapeutic potential lapatinib, dual kinase inhibitor. Using qPCR, cell culture assays, Western blotting, vivo models, we found significant upregulation...
Abstract A novel synthetic triterpenoid, 2-cyano-3,12-dioxooleana-1,9-dien-28-oic acid (CDDO), previously reported to have potent differentiating, antiproliferative, and antiinflammatory activities, has been identified as a ligand for the peroxisome proliferator-activated receptor γ (PPARγ). CDDO induces adipocytic differentiation in 3T3-L1 cells, although it is not full agonist of PPARγ, rosiglitazone. Binding studies PPARγ using scintillation proximity assay give Ki between 10−8 10−7m. In...
Non-canonical Wnt/planar cell polarity (PCP) signaling plays a primary role in the convergent extension that drives neural tube closure and body axis elongation. PCP gene mutations cause severe defects (NTDs). However, of canonical Wnt/β-catenin NTDs remains poorly understood. This study shows conditional targeting β-catenin dorsal folds mouse embryos represses expression homeobox-containing genes Pax3 Cdx2 at posterior neuropore (PNP), subsequently diminishes target T, Tbx6 Fgf8 tail bud,...
Surfactin is a cyclic lipopeptide that of great industrial use owing to its extraordinary surfactant power and antimicrobial, antiviral, antitumor activities. synthesized by condensation reaction in microbes, which uses fatty acids four kinds amino (l-glutamate, l-aspartate, l-leucine l-valine) as precursors. biosynthesis could be improved increasing the supply acids; however, effect regulation acid metabolism on surfactin production was not yet clear. In this study, we aimed improve B....
We initially randomly synthesized about 60 oleanane and ursane triterpenoids as potential anti-inflammatory cancer chemopreventive agents. Preliminary screening of these derivatives for inhibition production nitric oxide induced by interferon-γ in mouse macrophages revealed that 3-oxooleana-1,12-dien-28-oic acid (B-15) showed significant activity (IC50 = 5.6 μM). On the basis structure B-15, 19 novel olean- urs-12-ene with a 1-en-3-one functionality having substituent at C-2 ring A have been...
The scaffolding adapter Gab2 mediates cell signaling and responses evoked by various extracellular stimuli including several growth factors. Kit, the receptor for stem factor (SCF), plays a critical role in proliferation differentiation of variety types, mast cells. via Tyr(567) Tyr(719), activates Src family kinases (SFK) PI3K respectively, which converge on activation Rac/JNK pathway required proliferation. However, how Kit signals to is not well understood. By analyzing Gab2(-/-) cells,...