A5018927418- Xenotransplantation and immune response
- Virus-based gene therapy research
- Hepatitis Viruses Studies and Epidemiology
- Animal Genetics and Reproduction
- Viral gastroenteritis research and epidemiology
- Liver Disease and Transplantation
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Animal Virus Infections Studies
- Organ Transplantation Techniques and Outcomes
- Animal Disease Management and Epidemiology
- Veterinary medicine and infectious diseases
- CAR-T cell therapy research
- Pancreatic function and diabetes
- Viral Infections and Immunology Research
- Hepatitis C virus research
- T-cell and Retrovirus Studies
- Organ and Tissue Transplantation Research
- Herpesvirus Infections and Treatments
- Immunotherapy and Immune Responses
- Lymphoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Cancer Research and Treatments
- Liver Diseases and Immunity
- Cytomegalovirus and herpesvirus research
Glasgow Caledonian University
2016-2025
Johns Hopkins University
2024
Doctors Hospital
2024
Oncotherapeutics (United States)
2024
Massachusetts General Hospital
2024
Grantham University
2024
Dartmouth College
2024
University of Fribourg
2023
Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
2023
The University of Sydney
2023
Xenotransplantation carries the potential risk of transmission infection with cells or tissues graft. The degree is unknown in absence clinical trials. application xenotransplantation has important implications for infectious disease surveillance, both at national and international levels. Preclinical data indicate that events associated from swine, should they occur, will be rare; human trials are limited but have demonstrated no porcine microorganisms including endogenous retrovirus....
Published prevalence figures for hepatitis E virus (HEV) reveal significant regional differences. Several studies have reported transmission via blood transfusion. The aim of this study was to establish HEV seroprevalence and investigate a potential RNA presence in Scottish donors.IgG IgM were determined individual serum samples. investigated plasma mini-pools corresponding 43 560 donations using nested PCR. Samples amenable reamplification with primers from different region considered...
Acellular materials of xenogenic origin are used worldwide as xenografts, and phase I trials viable pig pancreatic islets currently being performed. However, limited information is available on transmission porcine endogenous retrovirus (PERV) after xenotransplantation the long-term immune response recipients to xenoantigens. We analyzed blood burn patients who had received living pig-skin dressings for up 8 wk presence PERV well level nature their long term (maximum, 34 y) against Ags....
ABSTRACT The replication of porcine endogenous retrovirus subgroup A (PERV-A) and PERV-B in certain human cell lines indicates that PERV may pose an infectious risk clinical xenotransplantation. We have previously reported human-tropic PERVs isolated from infected cells following cocultivation with miniature swine peripheral blood mononuclear (PBMC) are recombinants PERV-A PERV-C. Here, we report these exogenous viruses swine; i.e., they not present the germ line DNA. These were invariably...
Summary Background In developed countries, hepatitis E is a porcine zoonosis caused by virus ( HEV ) genotype 3. developing mainly 1, and causes increased mortality in patients with pre‐existing chronic liver disease CLD ). Aim To determine the role of decompensated . Methods Prospective testing 343 at three UK centres Toulouse France, follow‐up for 6 months or death. IgG seroprevalence was compared 911 controls. Results 11/343 (3.2%) had acute infection, died. There were no differences (27%...
REPORT (d) transgenic pig facilities; (e) biomaterials and encapsulation; (f) immunosuppression tolerance induction.The guidance document from the Second Global Consultation (Geneva, 2011) was also included in these discussions.
Background Previous studies showed low levels of circulating hepatitis E virus (HEV) in Scotland. We aimed to reassess current Scottish HEV epidemiology. Methods: Blood donor samples from five blood centres, the minipools for routine screening and liver transplant recipients were tested antibodies RNA determine seroprevalence viraemia. data compared with results previous covering 2004–08. Notified laboratory-confirmed cases (2009-16) extracted national surveillance data. Viraemic donors...
Shellfish samples (n = 310) purchased from local supermarkets were analysed for the presence of hepatitis E virus (HEV) by nested RT-PCR and real-time qRT-PCR. Overall, 2.9% tested positive HEV. Phylogenetic analysis HEV sequences revealed all as being genotype 3 This is first report detection in commercially sold shellfish Scotland. These findings may encourage further research that will help address gaps knowledge respect to foodborne transmission Scotland rest United Kingdom.
Abstract Chapter 2 of the original consensus statement published in 2009 by IXA represents an excellent basis for production safe donor pigs and pig‐derived materials porcine islet xenotransplantation. It was intended that to be reviewed at interval remain relevant. Indeed, many salient points relevant today, especially when xenotransplantation is performed conjunction with immunosuppressants. However, progress field including demonstrated clinical xenograft studies, increased understanding...
SUMMARY The prevalence of anti-HEV isotype-specific antibodies and viraemia were investigated in serum samples collected from slaughter-age pigs (aged 22–24 weeks) 23 farms Scotland. Of 176 tested, 29·0% ( n = 51) IgG positive, 36·9% 65) IgA positive IgM positive. Overall seroprevalence (anti-HEV IgG+ and/or IgA+ IgM+) was 61·4% 108). HEV RNA detected 72/162 (44·4%). Partial sequence ORF2 (98 nt) obtained eight RNA-positive phylogenetic analysis confirmed that they all genotype 3. This is...
Abstract Xenotransplantation of porcine cells, tissues, and organs may be associated with the transmission microorganisms to human recipient. A previous, 2009, version this consensus statement focused on strategies prevent endogenous retroviruses ( PERV s). This addresses potential all including monitoring recipient provides suggested approaches prevention disease transmission. Prior analyses assumed that most other than could eliminated from donor animals under appropriate conditions which...
Abstract Costimulatory and coinhibitory receptor–ligand pairs on T cells APC control the immune response. We have investigated whether selective blockade of CD28–CD80/86 costimulatory interactions, which preserves CTLA4–CD80/86 interactions function regulatory (Treg) cells, abrogates induction experimental autoimmune encephalomyelitis (EAE) in rhesus monkeys. EAE was induced by intracutaneous immunization with recombinant human myelin oligodendrocyte glycoprotein (rhMOG) CFA day 0. FR104 is...
ABSTRACT The potential transmission of porcine endogenous retroviruses (PERVs) has raised concern in the development xenotransplantation products. Our previous studies have resulted identification animals within a research herd inbred miniature swine that lack capacity to transmit PERV human cells vitro. In contrast, other were capable transmission. PERVs transmitted are recombinants between PERV-A and PERV-C post-VRA region envelope (B. A. Oldmixon, J. C. Wood, T. Ericsson, Wilson, M. E....
ABSTRACT Xenotransplantation may bridge the widening gap between shortage of donor organs and increasing number patients waiting for transplantation. However, a major safety issue is potential cross-species transmission porcine endogenous retroviruses (PERV). This problem could be resolved if it possible to produce pigs that do not contain replication-competent copies this virus. In order determine feasibility this, we have determined potentially full-length PERV proviruses obtained data on...
In this study, we have exploited the power of insertional mutagenesis to elucidate tumor progression pathways in mice carrying two oncogenes (MYC/Runx2) that collaborate drive early lymphoma development. Neonatal infection these with Moloney murine leukemia virus resulted accelerated onset associated increases clonal complexity and lymphoid dissemination. Large-scale analysis retroviral integration sites tumors revealed a profound bias towards narrow range target genes, including Jdp2...