A5025344954- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- Xenotransplantation and immune response
- Reproductive Physiology in Livestock
- Renal and related cancers
- Ovarian function and disorders
- Genetic and phenotypic traits in livestock
- Effects and risks of endocrine disrupting chemicals
- Tissue Engineering and Regenerative Medicine
- Virus-based gene therapy research
- Prenatal Screening and Diagnostics
- Bioenergy crop production and management
- Carcinogens and Genotoxicity Assessment
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- Mesenchymal stem cell research
- Assisted Reproductive Technology and Twin Pregnancy
- Animal testing and alternatives
- Cancer Research and Treatments
- Genetics, Aging, and Longevity in Model Organisms
- Birth, Development, and Health
Avantea
2015-2024
Pontifícia Universidade Católica de São Paulo
2024
University of Bologna
2007-2014
Istituto Sperimentale Italiano Lazzaro Spallanzani
2002-2009
Centro Medicina della Riproduzione
1994-2008
Kyoto University
2006
Ludwig-Maximilians-Universität München
2006
University of Missouri
2006
Samsung (South Korea)
2004
Instituto Oncológico de Córdoba
2004
The large offspring syndrome (LOS) is observed in bovine and ovine following transfer of vitro-produced (IVP) or cloned embryos characterized by a multitude pathologic changes, which extended gestation length increased birthweight are predominant features. In the present study, we used blastocysts to analyze cellular parameters, i.e., number cells Day 7 size 12 elongating blastocysts, molecular relative abundance developmentally important genes: glucose transporter (Glut) 1, Glut-2, Glut-3,...
Differential speeds in biochemical reactions have been proposed to be responsible for the differences developmental tempo between mice and humans. However, underlying mechanism controlling species-specific kinetics remains determined. Using vitro differentiation of pluripotent stem cells, we recapitulated segmentation clocks diverse mammalian species varying body weight taxa: marmoset, rabbit, cattle, rhinoceros. Together with mouse human, clock periods six did not scale animal weight, but...
With only three living individuals left on this planet, the northern white rhinoceros ( Ceratotherium simum cottoni ) could be considered doomed for extinction. It might still possible, however, to rescue (sub)species by combining novel stem cell and assisted reproductive technologies. To discuss various practical options available us, we convened a multidisciplinary meeting under name “Conservation Cellular Technologies.” The outcome of proposed road map that, if successfully implemented,...
Abstract Background Glutaraldehyde fixation does not guarantee complete tissue biocompatibility in current clinical bioprosthetic heart valves ( BHV s). Particularly, circulating anti‐α G al human antibodies increase significantly from just 10 days after a implantation. The inactivation of such epitope should be mandatory to meet the requirements for perspectively safe application; nevertheless, its quantitative assessment commercially available s has never been carried out. Methods In this...
Abstract The northern white rhinoceros (NWR, Ceratotherium simum cottoni ) is the most endangered mammal in world with only two females surviving. Here we adapt existing assisted reproduction techniques (ART) to fertilize Southern White Rhinoceros (SWR) oocytes NWR spermatozoa. We show that can be repeatedly recovered from live SWR by transrectal ovum pick-up, matured, fertilized intracytoplasmic sperm injection and developed blastocyst stage vitro. Next, generate hybrid embryos vitro using...
In vitro gametogenesis, the process of generating gametes from pluripotent cells in culture, is a powerful tool for improving our understanding germ cell development and an alternative source gametes. Here, we induced primordial cell-like (PGCLCs) stem northern white rhinoceros (NWR), species which only two females remain, southern (SWR), closest to NWR. PGCLC differentiation SWR embryonic highly reliant on bone morphogenetic protein WNT signals. Genetic analysis revealed that SRY-box...
Blastocyst formation rates during horse embryo in vitro production (IVP) are disappointing, and embryos that blastulate culture fail to produce the characteristic vital glycoprotein capsule. The aim of this study was evaluate impact IVP on development capsule formation. were produced by intracytoplasmic sperm injection matured oocytes either synthetic oviduct fluid (SOF) or temporary transfer a ewe. Control flushed from uterus mares 6–9 days after ovulation. Embryo morphology evaluated with...
The objective of the present work was to investigate and clarify factors affecting efficiency somatic cell nuclear transfer (NT) in horse, including embryo reconstruction, vitro culture blastocyst stage, transfer, pregnancy monitoring production offspring. Matured oocytes, with zona pellucida or after removal, were fused cumulus cells, granulosa fetal adult fibroblasts, couplets cultured . Blastocyst development Day 8 varied significantly among donor cells (from 1.3% 16%, P < 0.05). In...
In brief To save endangered rhinoceros species, assisted reproductive technologies are warranted. We here report in vitro blastocyst generation of the Near-Threatened Southern white and, for first time, also technically Extinct Northern rhinoceros. Abstract The Anthropocene is marked by a dramatic biodiversity decline, particularly affecting family Rhinocerotidae. Three five extant species listed as Critically Endangered (Sumatran, Javan, black rhinoceros), one Vulnerable (Indian and only...
We have used leukocytes and oocytes from commercially slaughtered animals to clone a progeny tested Brown Swiss bull. Mononuclear cells were separated the heparinized blood of donor male on Histopaque gradient cryopreserved. The nuclei thawed directly microinjected into enucleated Holstein Friesian that subsequently activated. Development morula was 23% blastocysts 17%. Some cloned compacting morulae subjected second round nucleus transfer by fusion individual blastomeres oocytes. these...
In contrast to mice, in sheep no genome-wide demethylation of the paternal genome occurs within first postfertilization cell cycle. This difference could be due either an absence a demethylase activity that is present mouse ooplasm or increased protection methylated cytosine residues sperm. Here, we use interspecies intracytoplasmic sperm injection demonstrate DNA can demethylated oocytes. Surprisingly, also limited extent Our results suggest murine process facilitated by sperm-derived...
Mesenchymal stem cells (MSCs) reside in the bone marrow and have potential for multilineage differentiation, into bone, cartilage, fat, example. In this study, bovine porcine MSCs were isolated, cultured to determine their replication ability, differentiated with osteogenic medium 5-azacytine. Both undifferentiated electroporated virally transduced test efficiency of genetic modification maintainance differentiation ability thereafter. Nuclear transfer experiments carried out MSCs, both at...
The aim of this study was the development an alternative testing method based on human embryonic stem cells for prenatal developmental toxicity with particular emphasis early neural development. To purpose, we designed in vitro protocol generation rosettes, representing counterpart developing plate and tube, challenged complex cell model retinoic acid (RA), a well-known teratogenic agent. were exposed to different concentrations RA during process rosettes formation. Morphological molecular...