A5059183502- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Birth, Development, and Health
- Renal and related cancers
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- Reproductive Health and Technologies
- Genetic Syndromes and Imprinting
- Prenatal Screening and Diagnostics
- RNA modifications and cancer
- Pancreatic function and diabetes
- Chromatin Remodeling and Cancer
- Tissue Engineering and Regenerative Medicine
- Molecular Biology Techniques and Applications
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sperm and Testicular Function
- Metabolism and Genetic Disorders
- Plant Molecular Biology Research
- RNA and protein synthesis mechanisms
- Genetic and phenotypic traits in livestock
- Cancer-related gene regulation
Institut Cellule Souche et Cerveau
2016-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2016-2025
Inserm
2014-2025
Université Claude Bernard Lyon 1
2016-2025
École Nationale Vétérinaire d'Alfort
2004-2022
Université Paris-Saclay
2016-2022
Biologie de la Reproduction, Environnement, Epigénétique et Développement
2020-2022
Université de Versailles Saint-Quentin-en-Yvelines
2021-2022
Institut National de la Recherche Agronomique du Niger
2002-2019
Roslin Institute
2002-2019
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It was previously shown that fully grown ovarian germinal vesicle (GV) oocytes of adult mice exhibit several nuclear configurations differ essentially by the presence or absence a ring condensed chromatin around nucleolus. These have been termed, respectively, SN (surrounded nucleolus) and NSN (nonsurrounded nucleolus). Work from our other laboratories has revealed ultrastructural functional differences between these two configurations. The aims present study were 1) to analyze equilibrium...
Active demethylation of cytosine residues in the sperm genome before forming a functional zygotic nucleus is thought to be an important function oocyte cytoplasm for subsequent embryonic development mouse. Conversely, this event does not occur sheep or rabbit zygote and occurs only partially cow. The aim study was investigate effect limited methylation reprogramming normal embryo on somatic nuclei. Sheep fibroblast nuclei were demethylated after electrofusion with recipient oocytes undergo...
In mammals, totipotent embryos are formed by fusion of highly differentiated gametes. Acquisition totipotency concurs with chromatin remodeling parental genomes, changes in the maternal transcriptome and proteome, zygotic genome activation (ZGA). The inefficiency reprogramming somatic nuclei reproductive cloning suggests that intergenerational inheritance germline contributes to developmental proficiency after natural conception. Here we show Ring1 Rnf2 , components Polycomb-repressive...
In eukaryotes, the interphase nucleus is organized in morphologically and/or functionally distinct nuclear "compartments". Numerous studies highlight functional relationships between spatial organization of and gene regulation. This raises question whether principles exist and, if so, they are identical animal plant kingdoms. We addressed this issue through investigation three-dimensional distribution centromeres chromocenters. investigated five very diverse populations nuclei at different...
Abstract Background Embryonic development proceeds through finely tuned reprogramming of the parental genomes to form a totipotent embryo. Cells within this embryo will then differentiate and give rise all tissues new individual. Early embryonic thus offers particularly interesting system in which analyze functional nuclear organization. When organization higher-order chromatin structures, such as pericentromeric heterochromatin, was first analyzed mouse embryos, specific rearrangements were...
Maternal environment during early developmental stages plays a seminal role in the establishment of adult phenotype. Using rabbit model, we previously showed that feeding dams with diet supplemented 8% fat and 0.2% cholesterol (HH diet) from prepubertal period throughout gestation induced metabolic syndrome offspring. Here, examined effects HH on feto-placental phenotype at 28 days post-coïtum (term = 31 days) relation to earlier blastocyst (Day 6). At days, both male female fetuses were...
In contrast to mice, in sheep no genome-wide demethylation of the paternal genome occurs within first postfertilization cell cycle. This difference could be due either an absence a demethylase activity that is present mouse ooplasm or increased protection methylated cytosine residues sperm. Here, we use interspecies intracytoplasmic sperm injection demonstrate DNA can demethylated oocytes. Surprisingly, also limited extent Our results suggest murine process facilitated by sperm-derived...
Genome reprogramming in early mouse embryos is associated with nuclear reorganization and particular features such as the peculiar distribution of centromeric pericentric heterochromatin during first developmental stage. This zygote-specific organization could be observed both maternal paternal pronuclei after natural fertilization well embryonic stem (ES) cell nuclei transfer suggesting that this type was essential for subsequent development.Here, we show remodeling into a zygotic-like also...
Early embryonic development is characterized by dramatic changes in cell potency and chromatin organization. The role of histone variants the context remodeling during embryogenesis remains under investigated. In particular, nuclear distribution variant H2A.Z its modifications have not been examined. Here we investigated dynamics acetylation two other active marks, H3K9ac H3K36me3, throughout murine bovine pre-implantation development. We show that dynamic earliest stages mouse development,...
During the final step of oogenesis, oocyte nucleus is subject to large-scale modifications that correlate with transcriptional silencing. While oocytes dense chromatin around nucleolus are silent (SN, surrounded nucleolus), uncondensed (NSN, non-surrounded nucleolus) transcriptionally active. It believed epigenetic mechanisms participate in gene expression regulation could play a role this event. In context, we examined behaviour heterochromatin and related histone during NSN SN transition...
Background In the mouse zygote, DNA methylation patterns are heavily modified, and differ between maternal paternal pronucleus. Demethylation of genome has been described as an active replication-independent process, although mechanisms responsible for it remain elusive. Recently, 5-hydroxymethylcytosine suggested intermediate in this demethylation. Methodology/Principal Findings study, we quantified hydroxymethylation both pronuclei zygote during replication period examined their on...
Until now, no primate animals have been successfully cloned to birth with somatic cell nuclear transfer (SCNT) procedures, and little is known about the molecular events that occurred in reconstructed embryos during preimplantation development. In many SCNT cases, epigenetic reprogramming of donor nuclei after into enucleated oocytes was hypothesized be crucial reestablishment embryonic totipotency. present study, we focused on two major marks, DNA methylation histone H3 lysine 9 (H3K9)...
Even though it generates healthy adults, nuclear transfer in mammals remains an inefficient process. Mainly attributed to abnormal reprograming of the donor chromatin, this inefficiency may also be caused at least partly by a specific effect cloning technique which has not yet been well investigated. There are two main procedures for transferring nuclei into enucleated oocytes: fusion and piezoelectric microinjection, latter being used mostly mice. We have, therefore, decided compare quality...
Alterations to DNA methylation have been attributed in vitro culture and may affect normal embryo development. We chose analyze reprogramming the rabbit which, of species with delayed transcriptional activation embryonic genome, allows easy comparisons between vivo-developed (IVD) vitro-cultured (IVC) embryos. In this species, variations had not previously quantified, even IVD IVC embryos were recovered at 2, 4, 8 16-cell, morula blastocyst stages. Immunostaining for 5-methyl-cytidine...
Understanding the mechanism of resistance genes to reactivation will help improve success nuclear reprogramming. Using mouse embryonic fibroblast nuclei with normal or reduced DNA methylation in combination chromatin modifiers able erase H3K9me3, H3K27me3, and H2AK119ub1 from transplanted nuclei, we reveal basis for transcriptional reprogramming by oocyte factors. A majority is affected more than one type treatment, suggesting that can require repression through multiple epigenetic...
Mouse embryonic stem cells (ESCs) and epiblast (EpiSCs) represent naive primed pluripotency states, respectively, are maintained in vitro by specific signalling pathways. Furthermore, ESCs cultured serum-free medium with two kinase inhibitors (2i-ESCs) thought to be the ground naïve pluripotent state. Here, we present a comparative study of epigenetic transcriptional states pericentromeric heterochromatin satellite sequences found these states. We show that 2i-ESCs distinguished from other...