A5053482670- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Virus-based gene therapy research
- Viral-associated cancers and disorders
- Multiple Myeloma Research and Treatments
- Animal Disease Management and Epidemiology
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- T-cell and Retrovirus Studies
- Amino Acid Enzymes and Metabolism
- Vector-Borne Animal Diseases
- Animal Virus Infections Studies
- Immunotherapy and Immune Responses
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer, Hypoxia, and Metabolism
- Peptidase Inhibition and Analysis
- Advanced biosensing and bioanalysis techniques
- Ubiquitin and proteasome pathways
- SARS-CoV-2 and COVID-19 Research
- Microbial infections and disease research
- Viral Infections and Vectors
- Protein Degradation and Inhibitors
- Immune Cell Function and Interaction
- Neonatal Respiratory Health Research
University of Parma
2016-2025
Ospedale Antonio Cardarelli
2023-2024
Federico II University Hospital
2017-2022
Washington State University
2017-2021
University of Padua
2012
Ospedale di Parma
2009-2010
National Interuniversity Consortium of Materials Science and Technology
2006-2008
University of Liège
2006
University Hospital of Zurich
2005
Roslin Institute
1996-1997
Calix[n]arenes functionalized with guanidinium groups at the upper rim and alkyl chains lower bind to DNA, condense it, in some cases, promote cell transfection depending on their structure lipophilicity. Atomic force microscopy (AFM) studies indicate that upon DNA binding hydrophobic association of lipophilic cone calix[4]arenes drives formation intramolecular condensates, characterized by loops emerging from a dense core. Furthermore, hexyl octyl confer these calixarenes capabilities....
Cell-penetrating peptides are widely used as molecular transporters for the internalization inside cells of various cargo, including proteins and nucleic acids. A special role is played by arginine-rich oligoarginines covalently linked or simply mixed with cargo. Here we report cell-penetrating agents in which arginine units clustered on a macrocyclic scaffold. Instead using long peptides, four single were attached to either upper lower rim calix[4]arene, kept cone conformation building...
Osteoblast impairment occurs within multiple myeloma cell infiltration into the bone marrow. Canonical Wnt signaling activation in osteoprogenitor cells is involved osteoblast formation through stabilization of dephosphorylated beta-catenin and its nuclear translocation. The effects on human mesenchymal/osteoprogenitor are unclear. In 60 patients checked, we found that among inhibitors, Dickkopf-1 secreted frizzled-related protein-3 were produced by cells. However, although or marrow plasma...
Bovine postpartum uterine disease, metritis, affects about 40% of animals and is widely considered to have a bacterial aetiology. Although the γ-herpesvirus bovine herpesvirus 4 (BoHV-4) has been isolated from several outbreaks metritis or abortion, role viruses in endometrial pathology mechanisms viral infection cells are often ignored. The objectives present study were explore interaction, tropism outcomes BoHV-4 challenge stromal epithelial cells. Endometrial purified infected with...
Mesenchymal stem cells (MSCs) reside in the bone marrow and have potential for multilineage differentiation, into bone, cartilage, fat, example. In this study, bovine porcine MSCs were isolated, cultured to determine their replication ability, differentiated with osteogenic medium 5-azacytine. Both undifferentiated electroporated virally transduced test efficiency of genetic modification maintainance differentiation ability thereafter. Nuclear transfer experiments carried out MSCs, both at...
ADVERTISEMENT RETURN TO ISSUEPREVLetterNEXTMacrocyclic Nonviral Vectors: High Cell Transfection Efficiency and Low Toxicity in a Lower Rim Guanidinium Calix[4]areneValentina Bagnacani†, Francesco Sansone*†, Gaetano Donofrio‡, Laura Baldini†, Alessandro Casnati†, Rocco Ungaro†View Author Information Dipartimento di Chimica Organica e Industriale, Università Parma, V.le G. P. Usberti 17/a, 43100 Italy Consorzio INSTM, Via Giusti 9, 50121 Firenze, Italy, Salute Animale, del Taglio 8,...
Experimental infection with the gamma-herpesvirus bovine herpesvirus 4 (BoHV-4) rarely establishes disease, yet BoHV-4 is commonly associated uterine disease in cattle. Uterine involves co-infection bacteria such as Escherichia coli, which stimulate production of prostaglandin E(2) (PGE(2)) by endometrial cells. replication depends on immediate early 2 (IE2) gene transactivation and, present study, PGE(2), E. coli or its lipopolysaccharide upregulated IE2 promoter Bacterial important for disease.
Guanidinium groups were introduced through a spacer at the lower rim of calix[4]arenes in cone conformation to give new potential nonviral vectors for gene delivery. Several structural modifications explored, such as presence or absence macrocyclic scaffold, lipophilicity backbone, length spacer, and nature charged groups, order better understand factors which affect DNA condensation ability transfection efficiency these derivatives. The most interesting compound was calix[4]arene...
Multi-head/multi-tail facial amphiphiles built on cyclodextrin (CD) and calixarene (CA) scaffolds are paradigmatic examples of monodisperse gene delivery systems. The possibility to precisely control the architectural features at molecular level offers unprecedented opportunities for conducting structure-activity relationship studies. A major requirement those channels is design a sufficiently diverse ensemble compounds parallel evaluation their capabilities condense DNA into transfection...
Abstract We studied in vivo the potential involvement of nuclear factor‐ κ B ( NF ‐ B) pathway molecular mechanism anti‐inflammatory and immunomodulatory activity azithromycin lung. Mice transiently transfected with luciferase gene under control a responsive element were used to assess activation by bioluminescence imaging. Bioluminescence as well inflammatory cells concentrations proinflammatory cytokines bronchoalveolar lavage fluids, monitored an acute model pulmonary inflammation...
COVID-19 is an ongoing pandemic caused by the highly infectious coronavirus SARS-CoV-2 that engaging worldwide scientific research to find a timely and effective eradication strategy. Great efforts have been put into anti-COVID-19 vaccine generation in effort protect world population block spread. To validate protective efficacy of vaccination campaign effectively control pandemic, it necessary quantify induction neutralizing antibodies vaccination, as they established be correlate...
Prion diseases are characterized by the deposition of PrP(Sc), an abnormal form cellular prion protein PrP(C). A growing body evidence suggests that antibodies to PrP(C) can antagonize PrP(Sc). However, host tolerance hampers induction immune responses PrP(C), and cross-linking bivalent anti-PrP is neurotoxic. In order obviate these problems, we explored antiprion potential recombinant single-chain antibody (scFv) fragments. scFv fragments derived from monoclonal 6H4, flagged with c-myc His6...
Monkeypox virus (MPXV) is the etiological agent of human (MPX). It an emerging orthopoxvirus zoonosis in tropical rain forest Africa and endemic Congo-basin sporadic West Africa; it remains a neglected disease persons impoverished rural areas. Interaction population with wildlife increases infection MPX (MPXV), from to possible. Smallpox vaccination provides good cross-protection against MPX; however, campaign ended 1980, meaning that large proportion currently unprotected MPXV infection....
Nipah virus (NiV) is an emergent pathogen capable of causing acute respiratory illness and fatal encephalitis in pigs humans. A high fatality rate broad host tropism makes NiV a serious public animal health concern. There therefore urgent need for vaccines to protect animals In this study we investigated the immunogenicity bovine herpesvirus (BoHV-4) vectors expressing either attachment (G) or fusion (F) glycoproteins, BoHV-4-A-CMV-NiV-GΔTK BoHV-4-A-CMV-NiV-FΔTK, respectively pigs. The were...
Scope Amyloid‐β oligomers (AβO) are causally related to Alzheimer's disease (AD). Dietary natural compounds, especially flavonoids and flavan‐3‐ols, hold great promise as potential AD‐preventive agents but their host gut microbiota metabolism complicates identification of the most relevant bioactive species. This study aims investigate ability a comprehensive set phenyl‐γ‐valerolactones (PVL), main circulating metabolites flavan‐3‐ols dietary compounds in humans, prevent AβO‐mediated...
The development of new drugs for idiopathic pulmonary fibrosis strongly relies on preclinical experimentation, which requires the continuous improvement animal models and integration with in vivo imaging data. Here, we investigated lung distribution bleomycin (BLM) associated indocyanine green (ICG) dye by fluorescence imaging. A long-lasting retention (up to 21 days) was observed upon oropharyngeal aspiration (OA) either ICG or BLM + ICG, significantly more severe fibrosis, accompanied...