A5023346003- Cytomegalovirus and herpesvirus research
- Viral-associated cancers and disorders
- Herpesvirus Infections and Treatments
- Polyomavirus and related diseases
- Parvovirus B19 Infection Studies
- Hematopoietic Stem Cell Transplantation
- Animal Virus Infections Studies
- T-cell and B-cell Immunology
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- HIV Research and Treatment
- Immune Cell Function and Interaction
- Viral Infections and Immunology Research
- Toxoplasma gondii Research Studies
- SARS-CoV-2 and COVID-19 Research
- Hepatitis C virus research
- Immunotherapy and Immune Responses
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Mycobacterium research and diagnosis
- Multiple Sclerosis Research Studies
- HIV/AIDS drug development and treatment
- Chronic Lymphocytic Leukemia Research
- Cutaneous lymphoproliferative disorders research
- Congenital Anomalies and Fetal Surgery
Nantes Université
2008-2024
Center for Research in Transplantation and Translational Immunology
2024
Inserm
1994-2023
Centre Hospitalier Universitaire de Nantes
2009-2021
Institut de Transplantation Urologie en Nephrologie
2016-2017
Centre Pays de la Loire
2013-2014
Université Nantes Angers Le Mans
2013
Laboratoire de Génétique Cellulaire
2013
Centre d'Investigation Clinique de Nantes
2013
Service de la Santé Publique
2013
BK virus (BKV) infection during the first year after renal transplantation was studied prospectively in 104 unselected consecutive patients. Viral DNA urine (DNAuria) and plasma (DNAemia) samples detected quantified by real-time PCR. The noncoding control region (NCCR) of BKV isolates sequenced. DNAuria DNAemia occurred 57% 29% patients, respectively. Three groups were defined, uninfected patients (group 1, n=45), with 2, n=29) positive 3, n=30). Active started within 3 months 80% Cold...
Cytomegalovirus (CMV) drug resistance is a therapeutic challenge in the transplant setting. No longitudinal cohort studies of CMV real-life setting have been published valganciclovir era. We report findings for French multicentre prospective 346 patients enrolled at initial diagnosis infection (clinical trial registered clinicaltrials.gov: NCT01008540). Patients were monitored detection ≥2 years. Real-time by UL97 and UL54 gene sequencing antiviral phenotyping was performed if viral...
Attachment to carbohydrates of the histo-blood group type several human Rotavirus strains (RVA) has recently been described. Synthesis these ligands requires a functional FUT2 enzyme, suggesting that null homozygote (ie, nonsecretor) individuals may not be recognized by most RVA strains. Whereas such represent 20% control population, this retrospective study determined none 51 patients infected P[8] rotavirus were nonsecretors. The lack α1,2fucosylated carbohydrate motifs in gut surface...
BackgroundChildren are underrepresented in the COVID-19 pandemic and often experience milder disease than adolescents adults. Reduced severity is possibly due to recent more frequent seasonal human coronaviruses (HCoV) infections. We assessed seroprevalence of SARS-CoV-2 HCoV specific antibodies a large cohort north-eastern France.MethodsIn this cross-sectional study, serum samples were collected from children adults requiring hospital admission for non-COVID-19 between February August 2020....
Human herpesvirus (HHV)–6 and HHV–7 loads were evaluated retrospectively in peripheral blood mononuclear cells (PBMC) from 78 recipients of stem cell transplantation (SCT) by real-time polymerase chain reaction. The median HHV-6 load patients was 1357 genome equivalent copies (EqCop)/106 PBMC but below the quantitation threshold 31 immunocompetent individuals, which strongly suggests that reactivation occurred after SCT. higher with delayed neutrophil engraftment (P=.002) or severe...
To determine the incidence and clinical relevance of active human herpesvirus 6 (HHV-6) infection, 92 consecutive unselected recipients autologous or allogeneic stem cell grafts were investigated in a prospective longitudinal study. Active infection was assessed by presence viral deoxyribonucleic acid (DNA) 846 peripheral blood mononuclear specimens 115 plasma specimens, means specially developed polymerase chain reaction designed to avoid detection latent genome. The HHV-6 observed 42.5%,...
CMV infection represents a major complication in hematopoietic stem cell transplantation, which compromises graft outcome. Downregulation of HLA class I expression is one mechanism by evades T cell-mediated immune detection, rendering infected cells vulnerable to killer Ig-like receptor (KIR)(+) NK cells. In this study, we observed that the amplified NKG2C(+) population specifically seropositive individuals mainly expressed KIR2DL receptors. We have shown was downregulated on CMV-infected...
ABSTRACT Cytotoxic T lymphocytes (CTLs) play a central role in the control of persistent human cytomegalovirus (HCMV) infection healthy virus carriers. Previous analyses specificity HCMV-reactive CD8 + CTLs drawn from vitro models which antigen-presenting cells were autologous fibroblasts infected with laboratory HCMV strains have shown focusing CTL responses against major tegument protein, pp65. By contrast, 72-kDa immediate-early protein (IE1) was identified as minor target for this...
Abstract Background Critical illness induces immune disorders associated with an increased risk of hospital-acquired pneumonia (HAP) and acute respiratory distress syndrome (ARDS). Torque teno virus (TTV), from the Anelloviridae family, is proposed as a biomarker to measure level immunosuppression. Our objective was describe kinetics TTV DNA loads their association critical illness–related complications. Methods We performed longitudinal study in 115 patients brain injury prospective cohort,...
Background. The purpose of this prospective study was to evaluate the usefulness quantifying DNA-cytomegalovirus (CMV) load for diagnosis and monitoring CMV disease among renal pancreas transplant patients under immunosuppressive drugs. Methods. A longitudinal conducted 34 consecutive, unselected pancreas/renal transplanted in our unit. During first 3 posttransplant months, weekly infection done, involving determination viremia by shell vial assay, qualitative DNAemia semi-nested polymerase...
The etiology of multiple sclerosis (MS) remains elusive. Among the possible causes, increase anti-Neu5Gc antibodies during EBV primo-infection Infectious mononucleosis (IMN) may damage integrity blood-brain barrier facilitating transfer EBV-infected B cells and anti-EBV T cell clones in brain. We investigated change titers anti-α1,3 Galactose 49 IMN, 76 MS, 73 clinically isolated syndrome (CIS) patients, as well age/gender-matched healthy individuals. Anti-Gal are significantly increased IMN...
Abstract Background The seasonal human coronaviruses (HCoV) NL63, 229E, OC43, and HKU1 are globally endemic, yet the majority of HCoV infections remain undiagnosed. Methods In a cross-sectional study, 2389 serum samples were collected from children adults in France 2020. longitudinal cohort 2520 898 French individuals followed up between 2020 2021. Antibodies to HCoVs measured using bead-based multiplex assay. Results rate waning anti-HCoV spike immunoglobulin G antibodies was estimated as...
Abstract Enteroviruses (EVs) and human herpesviruses (HHVs) are involved frequently in acute neurological disorders of viral etiology. This study aimed to investigate the incidence herpes simplex virus types‐1 (HSV‐1) 2 (HSV‐2), varicella‐zoster (VZV), cytomegalovirus (CMV), herpesvirus 6 (HHV‐6) enteroviruses cerebrospinal fluid (CSF) samples Tunisian immunocompetent patients with neuromeningeal disorders. The had been hospitalized at Fattouma Bourguiba University Hospital (Monastir,...
Two quantitative duplex real-time PCR assays were developed for co-amplification of human albumin and cytomegalovirus (CMV) or Epstein Barr virus (EBV) genes after automated extraction on whole blood, compared two units expressing viral DNA loads (copies per ml blood 10(6) peripheral leukocytes (PBLs)) 1,138 positive samples. Both PCRs characterized by high sensitivity, reproducibility, linear range. Automated a MagNA Pure LC Instrument was shown to be more efficient when cell count inferior...
The objective was to develop a method of HCV genome sequencing that allowed simultaneous genotyping and NS5A inhibitor resistance profiling. In order validate the use unique RT-PCR for genotypes 1–5, 142 plasma samples from patients infected with were analysed. NS4B-NS5A partial region successfully amplified sequenced in all samples. parallel, NS3 sequences analyzed obtained genotyping. Phylogenetic analysis showed concordance subtypes bootstrap >95% each type cluster. mutations using...
Primary human cytomegalovirus (HCMV) infection usually goes unnoticed, causing mild or no symptoms in immunocompetent individuals. However, some rare severe clinical cases have been reported without investigation of host immune responses viral virulence. In the present study, we investigate for first time phenotypic and functional features, together with gene expression profiles adults experiencing a primary HCMV infection. Twenty HCMV-infected patients (PHIP) were enrolled, as well 26...
The ZEBRA protein (encoded by the BZLF1 gene), is major transcription factor of EBV, expressed upon EBV lytic cycle activation. Several studies highlighted critical role infection as a risk for lymphoproliferative disorders like post-transplant disease (PTLD). Here, we use an antigen-capture ELISA assay specifically designed to detecting circulating soluble (sZEBRA) in serum samples (threshold value determined at 40ng/mL). We retrospectively investigated population 66 transplanted patients...
Cytomegalovirus (CMV) antigenemia was directly detected in polymorphonuclear leukocytes (PMNLs) from transplant recipients by using flow cytometry (FC). Two fixation and permeabilization methods seven anti-CMV monoclonal antibodies (MAbs) were evaluated. 1C3, SL20, NEA-9221 MAbs more efficacious. The detection threshold of FC 0.05% positive PMNLs, percentages correlated well with DNA viral load the appearance clinical symptoms.
Human herpesvirus 6 (HHV-6) replication was evaluated during in vitro expansion of CD34-positive cells that were selected from 11 peripheral blood progenitor cell (PBPC) samples. In order to permit cellular differentiation towards the myeloid lineage, PBPCs cultured for 14-21 days a liquid, serum-free medium supplemented with interleukin 1 (IL1), IL3, IL6, granulocyte-macrophage colony-stimulating factor, granulocyte factor and stem-cell factor. Among 10 cultures HHV-6-seropositive patients,...
Background Studies comparing cell components of blood and graft sources are very scarce. We present here a thorough study examining the cellular content various therapy products. Study Design Methods have prospectively compared by fluorescence‐activated sorting analyses composition three on one hand—peripheral (PB; n = 10) versus granulocyte–colony‐stimulating factor ( G ‐ CSF )‐mobilized PB GCSF‐PB , cord CB 10)—and other hand—unmanipulated bone marrow uBM 5) leukapheresis product LP thawed...