A5043055527- Reproductive Biology and Fertility
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Immune Cell Function and Interaction
- Advanced Fluorescence Microscopy Techniques
- Molecular Biology Techniques and Applications
- Gene expression and cancer classification
- Adenosine and Purinergic Signaling
- Mesenchymal stem cell research
- Axon Guidance and Neuronal Signaling
- Biomarkers in Disease Mechanisms
- Reproductive Health and Technologies
- Assisted Reproductive Technology and Twin Pregnancy
- Extracellular vesicles in disease
- Prenatal Screening and Diagnostics
- Muscle Physiology and Disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Heme Oxygenase-1 and Carbon Monoxide
- Immune responses and vaccinations
- Immunotherapy and Immune Responses
- Angiogenesis and VEGF in Cancer
- Renal and related cancers
- Renal Transplantation Outcomes and Treatments
- Cell Image Analysis Techniques
- Phagocytosis and Immune Regulation
Nantes Université
2019-2024
Inserm
2019-2024
Centre National de la Recherche Scientifique
2019-2024
Centre Hospitalier Universitaire de Nantes
2020-2022
Institut de Transplantation Urologie en Nephrologie
2019-2020
Center for Research in Transplantation and Translational Immunology
2020
Abstract STUDY QUESTION Is it possible to develop an automated annotation tool for human embryo development in time-lapse devices based on image analysis? SUMMARY ANSWER We developed and validated software the of morphokinetic parameters, having a good concordance with expert manual 701 videos. WHAT IS KNOWN ALREADY Morphokinetic parameters obtained are increasingly used assessment quality. However, their is time-consuming can be slightly operator-dependent, highlighting need fully...
One of the most common treatments for infertile couples is In Vitro Fertilization (IVF). It consists controlled ovarian hyperstimulation, followed by ovum pickup, fertilization, and embryo culture 2–6 days under environmental conditions, leading to intrauterine transfer or freezing embryos identified as having a good implantation potential embryologists. To allow continuous monitoring development, Time-lapse imaging incubators (TLI) were first released in IVF market around 2010. This...
The mechanisms regulating CD8
Abstract Background & Aims Cystic fibrosis (CF) is considered a multisystemic disorder in which CF‐associated liver disease (CFLD) the third most common cause of mortality. Currently, no effective treatment available for CFLD because its pathophysiology still unclear. Interestingly, exhibits identical vascular characteristics as non‐cirrhotic portal hypertension, recently classified porto‐sinusoidal disorders (PSVD). Methods Since endothelial cells (ECs) are an important component PSVD,...
Intracellular ion fluxes emerge as critical actors of immunoregulation but still remain poorly explored. In this study, we investigated the role redundant cation channels TMEM176A and TMEM176B (TMEM176A/B) in retinoic acid-related orphan receptor γt+ cells conventional dendritic (DCs) using germline conditional double knockout mice. Although Tmem176a/b appeared surprisingly dispensable for protective function Th17 group 3 innate lymphoid intestinal mucosa, found that they were required DCs...
Abstract Recent technological advances such as single-cell RNAseq 1-3 and CRISPR-CAS9-mediated knock-out 4 have allowed an unprecedented access into processes orchestrating human preimplantation development 5 . However, the sequence of events which occur during are still unknown. In particular, timing first lineage specification, process by morula cells acquire a specific fate, remains elusive. Here, we present model based on transcriptomic pseudotime modelling scRNAseq biologically...
Duchenne muscular dystrophy (DMD) is a neuromuscular disease caused by mutations in the gene encoding dystrophin. It leads to repeated cycles of muscle fiber necrosis and regeneration progressive replacement fibers fibrotic adipose tissue, with consequent weakness premature death. Fibrosis and, particular, collagen accumulation are important pathological features dystrophic muscle. A better understanding development fibrosis crucial enable management DMD. Three-dimensional (3D)...
Abstract Serial block face imaging (SFBI) is a method used to generate 3-dimensional (3D) reconstruction of sample via serial image acquisition. Several SBFI approaches have been proposed for large samples, differing in the ability contrast as well nature detected signal. We propose new system that detects endogenous autofluorescence signal paraffin-embedded samples. The preparation simplified compared other approaches, and adapted be integrated into routine histological preparation. More...
An important limitation to the development of Artificial Intelligence (AI)-based solutions for In Vitro Fertilization (IVF) is absence a public reference benchmark train and evaluate deep learning (DL) models. this work, we describe fully annotated dataset 704 videos developing embryos, total 337k images. We applied ResNet, LSTM, ResNet-3D architectures our demonstrate that they overperform algorithmic approaches automatically annotate stage phases. Altogether, propose first will allow...
Glioblastoma multiforme (GBM) is a rare, yet devastating, primary brain tumor in adults. Current treatments remain generally ineffective and GBM almost invariably recurs, resulting median survival of 15 months. This high malignancy sources notably from the resilience invasive capabilities cells. Within GBM, exists population self-sustaining transformed cells with stem-like properties (GSCs), which are thought to be responsible for initiation, growth, invasion, as well recurrence. In...
The common cellular origin between bone marrow adipocytes (BMAds) and osteoblasts contributes to the intimate link adipose tissue (BMAT) skeletal health. An imbalance differentiation ability of BMSCs towards one two lineages occurs in conditions like aging or osteoporosis, where mass is decreased. Recently, we showed that sodium-phosphate co-transporter PiT2/SLC20A2 an important determinant for mineralization, strength quality. Since reduced homozygous mutant mice, investigated this study...
Recent technological advances such as single-cell (sc) RNAseq and CRISPR-CAS9-mediated knock-out have allowed an unprecedented access to processes orchestrating human preimplantation development. However, the sequence of events which occur during development are still unknown. In particular, timing first lineage specification, process by morula cells acquire a specific fate, remains elusive. Here, we present mouse model based on transcriptomic pseudotime modelling scRNAseq biologically...
ABSTRACT The activation of mixed lineage kinase-like (MLKL) by receptor-interacting protein kinase-3 (RIPK3) controls the execution necroptosis, a regulated form necrosis that occurs in apoptosis-deficient conditions. Active oligomerized MLKL triggers exposure phosphatidylserine residues on cell surface and disrupts plasma membrane integrity forming lytic pores. also governs biogenesis shedding proinflammatory small extracellular vesicles (EVs) during early steps however molecular basis is...