A5010579196- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Nephrotoxicity and Medicinal Plants
- Agronomic Practices and Intercropping Systems
- Plant Disease Resistance and Genetics
- Drug-Induced Hepatotoxicity and Protection
- Nematode management and characterization studies
- Dialysis and Renal Disease Management
- Pharmacological Effects and Toxicity Studies
- Heavy Metals in Plants
- Coastal wetland ecosystem dynamics
- Renal Diseases and Glomerulopathies
- Microbial Metabolites in Food Biotechnology
- Electrolyte and hormonal disorders
- Cassava research and cyanide
- Chromosomal and Genetic Variations
- COVID-19 Clinical Research Studies
- Plant Molecular Biology Research
- Parathyroid Disorders and Treatments
- Genomics and Phylogenetic Studies
- Plant Pathogenic Bacteria Studies
- Chronic Kidney Disease and Diabetes
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- Nitric Oxide and Endothelin Effects
Laboratoire des Interactions Plantes Micro-Organismes
2013-2025
Centre National de la Recherche Scientifique
2005-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2022-2025
Epicura
2012-2023
University of Mons
2018-2023
Université de Toulouse
2018-2022
Génétique Physiologie et Systèmes d'Elevage
2022
Cliniques Universitaires Saint-Luc
2020
Directorate-General Joint Research Centre
2020
Université Libre de Bruxelles
2002-2015
Legumes can enter into symbiotic relationships with both nitrogen-fixing bacteria (rhizobia) and mycorrhizal fungi. Nodulation by rhizobia results from a signal transduction pathway induced in legume roots rhizobial Nod factors. DMI3 , Medicago truncatula gene that acts immediately downstream of calcium spiking this signaling is required for nodulation infection, has high sequence similarity to genes encoding calmodulin-dependent protein kinases (CCaMKs). This indicates likely an essential...
Rhizobial Nod factors induce in their legume hosts the expression of many genes and set motion developmental processes leading to root nodule formation. Here we report identification Medicago GRAS-type protein Nodulation signaling pathway 1 (NSP1), which is essential for all known factor-induced changes gene expression. NSP1 constitutively expressed, so it acts as a primary transcriptional regulator mediating responses, therefore, named factor response factor.
Legumes form symbiotic associations with both mycorrhizal fungi and nitrogen-fixing soil bacteria called rhizobia. Several of the plant genes required for transduction rhizobial signals, Nod factors, are also necessary symbiosis. Here, we describe cloning characterization one such gene from legume Medicago truncatula . The DMI1 (does not make infections) encodes a novel protein low global similarity to ligand-gated cation channel domain archaea. is highly conserved in angiosperms ancestral...
Rhizobium-induced root nodules are specialized organs for symbiotic nitrogen fixation. Indeterminate-type formed from an apical meristem and exhibit a spatial zonation which corresponds to successive developmental stages. To get dynamic integrated view of plant bacterial gene expression associated with nodule development, we used sensitive comprehensive approach based upon oriented high-depth RNA sequencing coupled laser microdissection regions. This study, focused on the association between...
Rhizobium meliloti produces lipochitooligosaccharide nodulation NodRm factors that are required for of legume hosts. O-acetylated and N-acylated by specific C16-unsaturated fatty acids. nodL mutants produce non-O-acetylated factors, nodFE with modified acyl substituents. Both exhibited a significantly reduced capacity to elicit infection thread (IT) formation in alfalfa. However, once initiated, ITs developed allowed the nitrogen-fixing nodules. In contrast, double nodF/nodL were unable...
Genome sequencing of the model legumes, Medicago truncatula and Lotus japonicus, provides an opportunity for large-scale sequence-based comparison two genomes in same plant family. Here we report synteny comparisons between these species, including details about chromosome relationships, blocks, microsynteny within genome regions lacking clear correspondence. The share a minimum 10 each with substantial collinearity frequently extending length whole arms. proportion genes syntenic collinear...
Chinese-herb nephropathy (CHN) is a rapidly progressive renal fibrosis associated with the intake of Chinese herb (Aristolochia fangchi) containing nephrotoxic and carcinogenic aristolochic acids (AA). This study attempted to reproduce main features human CHN (renal failure, tubular atrophy, interstitial fibrosis) in rat model similar that cyclosporin-induced nephropathy. Salt-depleted male Wistar rats received daily subcutaneous injections either 1 mg/kg body wt AA (low-dose group), 10...
Microsatellites are tandemly repeated short DNA sequences that favored as molecular-genetic markers due to their high polymorphism index. Plant genomes characterized date exhibit taxon-specific differences in frequency, genomic location, and motif structure of microsatellites, indicating extant microsatellites originated recently turn over quickly. With the goal using microsatellite integrate physical genetic maps Medicago truncatula, we surveyed frequency distribution perfect 77 Mbp...
A 6 kb DNA segment of the R. meliloti 2011 pSym megaplasmid, which contains genes controlling host specificity root hair infection and nodulation, was cloned sequenced. The sequence analysis, in conjunction with previous genetic data, allowed identification four nod qenes designated as E , F G H . nodH is divergently transcribed respect to nodFE nodG conserved nucleotide found around 200 bp upstream translation start nodF nodA This also present common species specific other Rhizobium...
Rhizobium meliloti nodulation (nod) genes required for specific infection and of alfalfa have been cloned. Transposon Tn5 mutagenesis defined three nod regions spanning 16 kilobases the pSym megaplasmid. Genetic cytological studies 62 nodulation-defective mutants allowed assignment symbiotic developmental phenotypes to common loci. Root hair curling was determined by both (region I) III) transcription units; locus IIIb (nodH gene) positively controlled on homologous host alfalfa, whereas...
Rhizobium nodFE Genes in the Biosynthesis of Nod Factors 20135genes, produces non-sulfated factors.The species-specific NodF and NodE proteins are homologous to acyl carrier /3-ketoacyl synthases which involved fatty acid synthesis (Shearman et al., 1986; Bibb 1989).In R. leguminosarum bv.viciae, protein is required for (2184 (2E,4E,GE,llZ-octadecatetraenoyl) moiety lipo-oligosaccharide molecule (Fig. 3, structure IIb).In meliloti, component
Aristolochic acid (AA), the plant extract of Aristolochia species, is involved in onset progressive tubulointerstitial renal fibrosis humans. Clinical and vitro findings have previously suggested that proximal tubule was target AA.Using a rat model AA nephropathy, tubular lesions induced by daily subcutaneous injections for 35 or 5 days were characterized biochemically histologically. Urinary excretion proteins, albumin, low molecular weight N-acetyl-beta-d-glucosaminidase, alpha-glutathione...
Legume roots show a remarkable plasticity to adapt their architecture biotic and abiotic constraints, including symbiotic interactions. However, global analysis of miRNA regulation in is limited, view the evolution miRNA-mediated diversification different ecotypes lacking. In model legume Medicago truncatula, we analyze small RNA transcriptome submitted pathogenic Genome mapping computational pipeline identify 416 candidates, known novel variants 78 families present miRBase. Stringent...
ObjectivesMany transplant physicians screen for and treat asymptomatic bacteriuria (ASB) during post-kidney-transplant surveillance. We investigated whether antibiotics are effective in reducing the occurrence of symptomatic urinary tract infection (UTI) kidney recipients with ASB.MethodsWe performed this multicentre, randomized, open-label trial who had ASB were ≥2 months post-transplantation. randomly assigned participants to receive or no therapy. The primary outcome was incidence UTI...
The Medicago truncatula DMI3 gene encodes a calcium- and calmodulin-dependent protein kinase (CCaMK) that is necessary for the establishment of both rhizobial mycorrhizal symbioses. two symbiotic signaling pathways diverge downstream DMI3; therefore, it has been proposed legumes have evolved particular form CCaMK, acting like switch able to discriminate between calcium signatures trigger appropriate pathway. To test this hypothesis, we examined whether CCaMK from nonlegume species was...
Summary Myc‐ LCO s are newly identified symbiotic signals produced by arbuscular mycorrhizal ( AM ) fungi. Like rhizobial Nod factors, they lipo‐chitooligosaccharides that activate the common signalling pathway CSSP in plants. To increase our limited understanding of roles we aimed to analyse ‐induced transcriptional changes and their genetic control. Whole genome RNA sequencing ‐seq) was performed on roots Medicago truncatula wild‐type plants, dmi3 nsp1 mutants affected nodulation...
The infectious processes of the Frankia-Alnus and Rhizobium-legume symbioses present strong similarities, suggesting existence analogies between Frankia root hair deforming factor rhizobia Nod factors. Biochemical functional were tested using ACoN24d strain. putative chitin-like nature was explored by (i) gas chromatography coupled to mass spectrometry thin layer chromatography, after radioactive labeling culture for detection chitin oligomers, (ii) following activity supernatant...
Background. The evidence base regarding the safety of intravenous (IV) iron therapy in patients with chronic kidney disease (CKD) is incomplete and largely based on small studies relatively short duration. Methods. FIND-CKD (ClinicalTrials.gov number NCT00994318) was a 1-year, open-label, multicenter, prospective study nondialysis-dependent CKD, anemia deficiency randomized (1:1:2) to IV ferric carboxymaltose (FCM), targeting higher (400–600 µg/L) or lower (100–200 ferritin, oral iron. A...