A5042280508- Plant Genetic and Mutation Studies
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant Surface Properties and Treatments
- Postharvest Quality and Shelf Life Management
- Plant Stress Responses and Tolerance
- Plant Physiology and Cultivation Studies
- Agricultural Practices and Plant Genetics
- Magnetic and Electromagnetic Effects
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Microbial infections and disease research
- Plant Gene Expression Analysis
- Plant Virus Research Studies
- Plant biochemistry and biosynthesis
- Legume Nitrogen Fixing Symbiosis
- Bacteriophages and microbial interactions
- Plant Micronutrient Interactions and Effects
- Plant nutrient uptake and metabolism
- GABA and Rice Research
- Greenhouse Technology and Climate Control
- Animal Virus Infections Studies
- Plant Pathogens and Fungal Diseases
- Plant Pathogens and Resistance
Biologie du Fruit et Pathologie
2014-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2004-2024
Université de Bordeaux
2011-2024
École Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine
2024
Unité de Recherche Œnologie
2016
Laboratoire de Biogenèse Membranaire
2013
Biopolymères Interactions Assemblages
2013
Laboratoire Physiologie Cellulaire & Végétale
2010
Centre National de la Recherche Scientifique
2006
Laboratoire des Interactions Plantes Micro-Organismes
2006
Legumes can enter into symbiotic relationships with both nitrogen-fixing bacteria (rhizobia) and mycorrhizal fungi. Nodulation by rhizobia results from a signal transduction pathway induced in legume roots rhizobial Nod factors. DMI3 , Medicago truncatula gene that acts immediately downstream of calcium spiking this signaling is required for nodulation infection, has high sequence similarity to genes encoding calmodulin-dependent protein kinases (CCaMKs). This indicates likely an essential...
To accelerate functional genomic research in tomato, we developed a Micro-Tom TILLING (Targeting Induced Local Lesions In Genomes) platform. DNA pools were constructed from 3,052 ethyl methanesulfonate (EMS) mutant lines treated with 0.5 or 1.0% EMS. The mutation frequency was calculated by screening 10 genes. 0.5% EMS population had mild of one per 1,710 kb, whereas the 737 suitable for producing an allelic series mutations target overall 1,237 which affected average three alleles kilobase...
The thick cuticle covering and embedding the epidermal cells of tomato (Solanum lycopersicum) fruit acts not only as a protective barrier against pathogens water loss but also influences quality traits such brightness postharvest shelf-life. In recent study, we screened mutant collection miniature cultivar Micro-Tom isolated several glossy mutants in which abundance cutin, polyester component cuticle, was strongly reduced. We employed newly developed mapping-by-sequencing strategy to...
Asexual and sexual reproduction occur jointly in many angiosperms. Stolons (elongated stems) are used for asexual the crop species potato (Solanum tuberosum) strawberry (Fragaria spp), where they produce tubers clonal plants, respectively. In strawberry, stolon production is essential vegetative propagation at expense of fruit yield, but underlying molecular mechanisms unknown. Here, we show that deficiency trait runnerless (r) natural mutant woodland diploid vesca) due to a deletion active...
Ascorbate (vitamin C) is an essential antioxidant in fresh fruits and vegetables. To gain insight into the regulation of ascorbate metabolism plants, we studied mutant tomato plants (Solanum lycopersicum) that produce ascorbate-enriched fruits. The causal mutation, identified by a mapping-by-sequencing strategy, corresponded to knock-out recessive mutation class photoreceptor named PAS/LOV protein (PLP), which acts as negative regulator biosynthesis. This trait was confirmed CRISPR/Cas9 gene...
The cuticle is a protective layer synthesized by epidermal cells of the plants and consisting cutin covered filled waxes. In tomato (Solanum lycopersicum) fruit, thick embedding has crucial roles in control pathogens, water loss, cracking, postharvest shelf-life, brightness. To identify mutants with modified composition architecture to further decipher relationships between fruit brightness tomato, we screened an ethyl methanesulfonate mutant collection miniature cultivar Micro-Tom for...
Ascorbate is a major antioxidant buffer in plants. Several approaches have been used to increase the ascorbate content of fruits and vegetables. Here, we combined forward genetics with mapping-by-sequencing using an ethyl methanesulfonate (EMS)-mutagenized Micro-Tom population identify putative regulators underlying high-ascorbate phenotype tomato fruits. Among ascorbate-enriched mutants, family highest fruit level (P17C5, up 5-fold wild-type level) had strongly impaired flower development...
Tomato (Solanum lycopersicum) is regarded as a model plant of the Solanaceae family. The genome sequencing tomato cultivar 'Heinz 1706' was recently completed. To accelerate progress genomics studies, systematic bioresources, such mutagenized lines and full-length cDNA libraries, have been established for 'Micro-Tom'. However, these resources cannot be utilized to their full potential without completion We undertook 'Micro-Tom' here report identification single nucleotide polymorphisms...
Cuticle function is closely related to the structure of cutin polymer. However, and formation this hydrophobic polyester glycerol hydroxy/epoxy fatty acids has not been fully resolved. An apoplastic GDSL-lipase known as CUTIN SYNTHASE1 (CUS1) required for deposition in tomato (Solanum lycopersicum) fruit exocarp. In vitro, CUS1 catalyzes self-transesterification 2-monoacylglycerol 9(10),16-dihydroxyhexadecanoic acid, major monomer. This reaction releases leads oligomers with secondary...
Underground water enriched in NaHCO3 is used farms of the Buenos Aires province for tomato crop irrigation. This farming practice leads to salt accumulation and soil impairment after several seasons cultivation inside greenhouses. work assayed effect on fruit production. Plants Elpida variety Solanum lycopersicum L were grown a hydroponic system. The presence (from 5 mM, as measured underground 10 or 20 mM) reduced K+/Na+ ratio whole plant biomass yield; however, no was observed quality...
Tocopherol, a compound with vitamin E (VTE) activity, is conserved constituent of the plastidial antioxidant network in photosynthetic organisms. The synthesis tocopherol involves condensation an aromatic head group isoprenoid prenyl side chain. latter, phytyl diphosphate, can be derived from chlorophyll phytol tail recycling, which depends on kinase (VTE5) activity. How plants co-ordinate precursor distribution for supplying biosynthesis and other prenyllipids different organs poorly...
Tomato is currently the model plant for fleshy fruit development and Solanaceae species. Recent genomic approaches including transcriptome, proteome metabolome analyses genetic mapping have produced a wealth of candidate genes whose function needs to be assessed. The recent in crop plants TILLING (Targeting Induced Local Lesions IN Genomes), which reveals allelic series corresponding several independent point mutations, current availability deep sequencing tools further increase interest...
Besides being a model for fleshy fruits and Solanaceae species, tomato represents one of the main sources ascorbate (vitamin C) in human diet many parts world. Ascorbate fulfills various roles plants due to its antioxidant potential connection with other metabolic pathways e.g. cell wall biosynthesis. Among functional genomic tools recently developed tomato, EMS (ethyl methanesulfonate) mutant collections provide an opportunity identifying allelic series mutations target genes by TILLING...
Tomato (Solanum lycopersicum) is an established model for studying plant cuticle because of its thick covering and embedding the epidermal cells fruit. In this study, we screened EMS mutant collection miniature tomato cultivar Micro-Tom fruit cracking mutants found a displaying glossy phenotype. By using mapping-by-sequencing strategy, identified causal mutation in SlSHN2 transcription factor that specifically expressed outer epidermis growing The point shn2 introduces K to N amino acid...