A5001808393- Plant Surface Properties and Treatments
- Postharvest Quality and Shelf Life Management
- Food composition and properties
- Tree Root and Stability Studies
- Wheat and Barley Genetics and Pathology
- Polyamine Metabolism and Applications
- Horticultural and Viticultural Research
- Plant Micronutrient Interactions and Effects
- Alkaline Phosphatase Research Studies
- Protein purification and stability
- Plant Virus Research Studies
- Protein Hydrolysis and Bioactive Peptides
- Animal health and immunology
- Xenotransplantation and immune response
- Plant biochemistry and biosynthesis
- Plant Reproductive Biology
- Phytase and its Applications
- Plant tissue culture and regeneration
- Proteins in Food Systems
- Polysaccharides and Plant Cell Walls
- Nanocomposite Films for Food Packaging
- Polysaccharides Composition and Applications
- Enzyme Structure and Function
- Insect Resistance and Genetics
- Seed Germination and Physiology
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2019-2024
Biopolymères Interactions Assemblages
2010-2024
Institut National de la Recherche Agronomique
2007-2015
Summary The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component the cutin polyester entangled with cell wall polysaccharides. nature of cutin‐embedded polysaccharides (CEPs) their association are still unresolved Using tomato fruit as a model, chemical enzymatic pretreatments combined biochemical biophysical methods were developed to compare fine structure CEPs that noncutinized (NCPs). In addition, we used fruits from...
Cuticle function is closely related to the structure of cutin polymer. However, and formation this hydrophobic polyester glycerol hydroxy/epoxy fatty acids has not been fully resolved. An apoplastic GDSL-lipase known as CUTIN SYNTHASE1 (CUS1) required for deposition in tomato (Solanum lycopersicum) fruit exocarp. In vitro, CUS1 catalyzes self-transesterification 2-monoacylglycerol 9(10),16-dihydroxyhexadecanoic acid, major monomer. This reaction releases leads oligomers with secondary...
With the aim of mimicking plant cell wall, a layer by approach was used to build thin film consisting successive adsorption pectin and extensin. Elaboration monitored surface plasmon resonance, quartz crystal microbalance, ellipsometry. All data indicate that formation successful growth occurred according nonuniform growth. It is likely diffusion polymers within multilayer structure final not constituted layered individual extensin films. Polymer rearrangements were also supported atomic...
Abstract The cuticle is a complex polymer matrix that protects all aerial organs of plants, fulfills multiple roles in plant–environment interactions, and critical for plant development. These functions are associated with the structural features cuticles, architectural modeling cuticles during development crucial understanding their physical properties biological functions. In this work, in-depth architecture cutin fruit was investigated. Using cherry tomato (Solanum lycopersicum) as model...
Summary Cuticles are multifunctional hydrophobic biocomposites that protect the aerial organs of plants. During plant development, cuticles must accommodate different mechanical constraints combining extensibility and stiffness, corresponding relationships with their architecture unknown. Recent data showed a fine‐tuning cuticle during fruit several chemical clusters which raise question how they impact properties cuticles. We investigated in‐depth nanomechanical tomato ( Solanum...
Grain hardness is an important quality trait of cereal crops. In wheat, it mainly determined by the Hardness locus that harbors genes encoding puroindoline A (PINA) and B (PINB). Any deletion or mutation these leading to absence PINA single amino acid changes in PINB leads hard endosperms. Although generally acknowledged controlled adhesion strength between protein matrix starch granules, physicochemical mechanisms connecting puroindolines starch-protein interactions are unknown as this...
Polyesters, as they exist in planta, are promising materials with which to begin the development of "green" replacements. Cutin and suberin, polyesters found ubiquitously plants, prime candidates. Samples enriched for plant polyesters, their native backbones were largely preserved, studied identify "natural" structural features; features that influence critical physical properties. Quantitative nuclear magnetic resonance (NMR), differential scanning calorimetry (DSC), X-ray scattering...
A monoclonal antibody based capture immunoassay has been recently developed for the specific quantitation of bovine milk alkaline phosphatase (ALP) without interference by contaminating microbial or fungal ALPs (Geneix et al. 2007). This was used to study kinetics ALP heat denaturation in over a range 50–60°C 5 60 min using colorimetric quantification enzyme activity as reference test. midpoint obtained at 56°C 30 heating. Thermal inactivation found follow first order and is characterized z...
One of the objectives malting industry is to reduce energy cost during kilning without major effect on malt quality. In this study, impact a low hydration steeping process lipid transfer protein (LTP1) modifications and β-glucan breakdown was evaluated in (LH) high (HH) hydrated malts. LTP1 analyzed by MS/MS revealed acylation, glycation, disulfide bond breakage both LH HH free amine content measurement fluorescence Maillard adducts no significant difference between Immunolabeling...
The detection of alkaline phosphatase (ALP) activity is used as a legal test to determine whether milk has been adequately pasteurized or recontaminated with raw milk. However, wide variety microorganisms produce both heat labile and stable ALPs which cannot be differentiated from the ALP by current enzymatic methods. Monoclonal antibodies specific bovine were obtained in mice preparation. Coated microtitre plates, these specifically capture dairy products. After washing, captured revealed...
Abstract The thick cuticle covering and embedding epidermal cells of tomato ( Solanum lycopersicum ) fruit, a model for studies, plays important roles in fruit protection quality. To further our understanding the influence components on architecture properties, next step is to engineer composition. Ideally, avoid indirect effects at whole-plant level, fruit-and epidermis-specific promoter should be used. Here, overexpress edit target genes tomato, we selected non-specific Lipid Transfer...
Abstract Grain hardness is an important quality trait of cereal crops. In wheat, it mainly determined by the Hardness locus that harbors genes encoding puroindoline A (PINA) and B (PINB). Any deletion or mutation these leading to absence PINA single amino acid changes in PINB leads hard endosperms. Although generally acknowledged controlled adhesion strength between protein matrix starch granules, physicochemical mechanisms connecting puroindolines starch-protein interactions are unknown as...
Abstract Cuticle are multifunctional hydrophobic biocomposites that protect aerial organs of plants. Along plant development, cuticle must accommodate different mechanical constraints combining extensibility and stiffness, the corresponding structure-function relationships unknown. Recent data showed a fine architectural tuning architecture chemical clusters along fruit development which raise question their impact on properties cuticle. We investigated in-depth nanomechanical tomato from...